Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl mice

Spontaneously hyperlipidaemic (Apoeshl) mice were discovered in 1999 as mice lacking apolipoprotein E (ApoE) owing to a mutation in the Apoe gene. However, age-related behavioural changes in commercially available Apoeshl mice have not yet been clarified. The behavioural abnormalities of ApoE-defici...

Full description

Saved in:
Bibliographic Details
Main Authors: Ueno Hiroshi, Takahashi Yu, Mori Sachiko, Kitano Eriko, Murakami Shinji, Wani Kenta, Miyazaki Tetsuji, Matsumoto Yosuke, Okamoto Motoi, Ishihara Takeshi
Format: Article
Language:English
Published: De Gruyter 2025-02-01
Series:Translational Neuroscience
Subjects:
Online Access:https://doi.org/10.1515/tnsci-2022-0363
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850240506794082304
author Ueno Hiroshi
Takahashi Yu
Mori Sachiko
Kitano Eriko
Murakami Shinji
Wani Kenta
Miyazaki Tetsuji
Matsumoto Yosuke
Okamoto Motoi
Ishihara Takeshi
author_facet Ueno Hiroshi
Takahashi Yu
Mori Sachiko
Kitano Eriko
Murakami Shinji
Wani Kenta
Miyazaki Tetsuji
Matsumoto Yosuke
Okamoto Motoi
Ishihara Takeshi
author_sort Ueno Hiroshi
collection DOAJ
description Spontaneously hyperlipidaemic (Apoeshl) mice were discovered in 1999 as mice lacking apolipoprotein E (ApoE) owing to a mutation in the Apoe gene. However, age-related behavioural changes in commercially available Apoeshl mice have not yet been clarified. The behavioural abnormalities of ApoE-deficient mice, which are genetically modified mice artificially deficient in ApoE, have been investigated in detail, and it has been reported that they can serve as a model of Alzheimer’s disease (AD). To understand whether Apoeshl mice can also serve as a murine model of AD, it is necessary to investigate age-related behavioural abnormalities in Apoeshl mice. In this study, we conducted a series of behavioural experiments on 7- and 11-month-old Apoeshl mice to investigate the behavioural abnormalities associated with ageing in Apoeshl mice. In this study, 7-month-old Apoeshl mice showed decreased body weight and grip strength compared to age-matched wild-type mice. In the open field test, 7-month-old Apoeshl mice showed increased anxiety-like behaviour compared to wild-type mice, whereas 11-month-old Apoeshl mice showed decreased anxiety-like behaviour. Moreover, Apoeshl mice aged 7 and 11 months had increased serum cholesterol levels. These results indicate that the behaviour of Apoeshl mice changes with age. However, 11-month-old Apoeshl mice did not show a decline in cognitive function or memory ability similar to murine models of AD. Our findings indicate that Apoeshl mice can be used to investigate the function of ApoE in the central nervous system.
format Article
id doaj-art-7eebf660d3d64f6fb61092c433d804c4
institution OA Journals
issn 2081-6936
language English
publishDate 2025-02-01
publisher De Gruyter
record_format Article
series Translational Neuroscience
spelling doaj-art-7eebf660d3d64f6fb61092c433d804c42025-08-20T02:00:51ZengDe GruyterTranslational Neuroscience2081-69362025-02-01161143526010.1515/tnsci-2022-0363Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl miceUeno Hiroshi0Takahashi Yu1Mori Sachiko2Kitano Eriko3Murakami Shinji4Wani Kenta5Miyazaki Tetsuji6Matsumoto Yosuke7Okamoto Motoi8Ishihara Takeshi9Department of Medical Technology, Kawasaki University of Medical Welfare, Okayama, 701-0193, JapanDepartment of Psychiatry, Kawasaki Medical School, Kurashiki, 701-0192, JapanDepartment of Psychiatry, Kawasaki Medical School, Kurashiki, 701-0192, JapanDepartment of Psychiatry, Kawasaki Medical School, Kurashiki, 701-0192, JapanDepartment of Psychiatry, Kawasaki Medical School, Kurashiki, 701-0192, JapanDepartment of Psychiatry, Kawasaki Medical School, Kurashiki, 701-0192, JapanDepartment of Psychiatry, Kawasaki Medical School, Kurashiki, 701-0192, JapanDepartment of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8558, JapanDepartment of Medical Technology, Graduate School of Health Sciences, Okayama University, Okayama, 700-8558, JapanDepartment of Psychiatry, Kawasaki Medical School, Kurashiki, 701-0192, JapanSpontaneously hyperlipidaemic (Apoeshl) mice were discovered in 1999 as mice lacking apolipoprotein E (ApoE) owing to a mutation in the Apoe gene. However, age-related behavioural changes in commercially available Apoeshl mice have not yet been clarified. The behavioural abnormalities of ApoE-deficient mice, which are genetically modified mice artificially deficient in ApoE, have been investigated in detail, and it has been reported that they can serve as a model of Alzheimer’s disease (AD). To understand whether Apoeshl mice can also serve as a murine model of AD, it is necessary to investigate age-related behavioural abnormalities in Apoeshl mice. In this study, we conducted a series of behavioural experiments on 7- and 11-month-old Apoeshl mice to investigate the behavioural abnormalities associated with ageing in Apoeshl mice. In this study, 7-month-old Apoeshl mice showed decreased body weight and grip strength compared to age-matched wild-type mice. In the open field test, 7-month-old Apoeshl mice showed increased anxiety-like behaviour compared to wild-type mice, whereas 11-month-old Apoeshl mice showed decreased anxiety-like behaviour. Moreover, Apoeshl mice aged 7 and 11 months had increased serum cholesterol levels. These results indicate that the behaviour of Apoeshl mice changes with age. However, 11-month-old Apoeshl mice did not show a decline in cognitive function or memory ability similar to murine models of AD. Our findings indicate that Apoeshl mice can be used to investigate the function of ApoE in the central nervous system.https://doi.org/10.1515/tnsci-2022-0363ageapolipoproteinbehavioural testcentral nervous systemmouse
spellingShingle Ueno Hiroshi
Takahashi Yu
Mori Sachiko
Kitano Eriko
Murakami Shinji
Wani Kenta
Miyazaki Tetsuji
Matsumoto Yosuke
Okamoto Motoi
Ishihara Takeshi
Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl mice
Translational Neuroscience
age
apolipoprotein
behavioural test
central nervous system
mouse
title Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl mice
title_full Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl mice
title_fullStr Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl mice
title_full_unstemmed Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl mice
title_short Age-related behavioural abnormalities in C57BL/6.KOR–Apoe shl mice
title_sort age related behavioural abnormalities in c57bl 6 kor apoe shl mice
topic age
apolipoprotein
behavioural test
central nervous system
mouse
url https://doi.org/10.1515/tnsci-2022-0363
work_keys_str_mv AT uenohiroshi agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT takahashiyu agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT morisachiko agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT kitanoeriko agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT murakamishinji agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT wanikenta agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT miyazakitetsuji agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT matsumotoyosuke agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT okamotomotoi agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice
AT ishiharatakeshi agerelatedbehaviouralabnormalitiesinc57bl6korapoeshlmice