Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas
Abstract Eukaryotic cells are highly compartmentalized, requiring elaborate transport mechanisms to facilitate the movement of proteins between membrane‐bound compartments. Most proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi apparatus through COPII‐mediated vesic...
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| Format: | Article |
| Language: | English |
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Wiley
2024-06-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.614 |
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| _version_ | 1832541414762741760 |
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| author | Kin Pan Chung Daniel Frieboese Florent Waltz Benjamin D. Engel Ralph Bock |
| author_facet | Kin Pan Chung Daniel Frieboese Florent Waltz Benjamin D. Engel Ralph Bock |
| author_sort | Kin Pan Chung |
| collection | DOAJ |
| description | Abstract Eukaryotic cells are highly compartmentalized, requiring elaborate transport mechanisms to facilitate the movement of proteins between membrane‐bound compartments. Most proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi apparatus through COPII‐mediated vesicular trafficking. Sar1, a small GTPase that facilitates the formation of COPII vesicles, plays a critical role in the early steps of this protein secretory pathway. Sar1 was characterized in yeast, animals and plants, but no Sar1 homolog has been identified and functionally analyzed in algae. Here we identified a putative Sar1 homolog (CrSar1) in the model green alga Chlamydomonas reinhardtii through amino acid sequence similarity. We employed site‐directed mutagenesis to generate a dominant‐negative mutant of CrSar1 (CrSar1DN). Using protein secretion assays, we demonstrate the inhibitory effect of CrSar1DN on protein secretion. However, different from previously studied organisms, ectopic expression of CrSar1DN did not result in collapse of the ER‐Golgi interface in Chlamydomonas. Nonetheless, our data suggest a largely conserved role of CrSar1 in the ER‐to‐Golgi protein secretory pathway in green algae. |
| format | Article |
| id | doaj-art-7ee20ea152404f5f85155812b7a1acc5 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-7ee20ea152404f5f85155812b7a1acc52025-02-04T08:35:53ZengWileyPlant Direct2475-44552024-06-0186n/an/a10.1002/pld3.614Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in ChlamydomonasKin Pan Chung0Daniel Frieboese1Florent Waltz2Benjamin D. Engel3Ralph Bock4Max‐Planck‐Institut für Molekulare Pflanzenphysiologie Potsdam GermanyMax‐Planck‐Institut für Molekulare Pflanzenphysiologie Potsdam GermanyBiozentrum University of Basel Basel SwitzerlandBiozentrum University of Basel Basel SwitzerlandMax‐Planck‐Institut für Molekulare Pflanzenphysiologie Potsdam GermanyAbstract Eukaryotic cells are highly compartmentalized, requiring elaborate transport mechanisms to facilitate the movement of proteins between membrane‐bound compartments. Most proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi apparatus through COPII‐mediated vesicular trafficking. Sar1, a small GTPase that facilitates the formation of COPII vesicles, plays a critical role in the early steps of this protein secretory pathway. Sar1 was characterized in yeast, animals and plants, but no Sar1 homolog has been identified and functionally analyzed in algae. Here we identified a putative Sar1 homolog (CrSar1) in the model green alga Chlamydomonas reinhardtii through amino acid sequence similarity. We employed site‐directed mutagenesis to generate a dominant‐negative mutant of CrSar1 (CrSar1DN). Using protein secretion assays, we demonstrate the inhibitory effect of CrSar1DN on protein secretion. However, different from previously studied organisms, ectopic expression of CrSar1DN did not result in collapse of the ER‐Golgi interface in Chlamydomonas. Nonetheless, our data suggest a largely conserved role of CrSar1 in the ER‐to‐Golgi protein secretory pathway in green algae.https://doi.org/10.1002/pld3.614Chlamydomonas reinhardtiiCOPII vesiclesER‐to‐Golgi protein traffickingprotein secretionSar1 |
| spellingShingle | Kin Pan Chung Daniel Frieboese Florent Waltz Benjamin D. Engel Ralph Bock Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas Plant Direct Chlamydomonas reinhardtii COPII vesicles ER‐to‐Golgi protein trafficking protein secretion Sar1 |
| title | Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas |
| title_full | Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas |
| title_fullStr | Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas |
| title_full_unstemmed | Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas |
| title_short | Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas |
| title_sort | identification and characterization of the copii vesicle forming gtpase sar1 in chlamydomonas |
| topic | Chlamydomonas reinhardtii COPII vesicles ER‐to‐Golgi protein trafficking protein secretion Sar1 |
| url | https://doi.org/10.1002/pld3.614 |
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