Multi-modal comparison of murine and human incisal dentin-enamel junctions
Human and mouse incisors are both primarily composed of dentin and enamel, which meet at an interface called the dentin-enamel junction (DEJ). However, incisors in the two species have very different growth patterns, structures, and loading requirements. Since the DEJ is responsible for minimizing c...
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Frontiers Media S.A.
2025-08-01
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| Series: | Acta Biochimica Polonica |
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| Online Access: | https://www.frontierspartnerships.org/articles/10.3389/abp.2025.14642/full |
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| author | Michael Truhlar Sobhan Katebifar Bradley Rosenberg Roland Kroger Alix C. Deymier |
| author_facet | Michael Truhlar Sobhan Katebifar Bradley Rosenberg Roland Kroger Alix C. Deymier |
| author_sort | Michael Truhlar |
| collection | DOAJ |
| description | Human and mouse incisors are both primarily composed of dentin and enamel, which meet at an interface called the dentin-enamel junction (DEJ). However, incisors in the two species have very different growth patterns, structures, and loading requirements. Since the DEJ is responsible for minimizing cracking at this at-risk interface between mechanically dissimilar dentin and enamel, its structure is expected to be significantly different between humans and mice. Here, structural and compositional gradients across human and murine incisors DEJs were measured via microcomputed tomography and Raman spectroscopy. Density gradients across the DEJ were significantly larger in humans compared to murine teeth, likely due to the larger size of the mantle dentin. Multiple gradients in mineral content and crystallinity were found at the murine DEJ, while the human DEJ only exhibited gradients in mineral content. Models predicting the modulus across the DEJ according to compositional results show that mineral crystallinity is critical in regulating gradients in tissue stiffness across the murine DEJ. Together, these results show the multiple ways in which the DEJ can adapt to variations in the loading environment. |
| format | Article |
| id | doaj-art-7ee1058b8569426cbbe2d0322fccf744 |
| institution | Kabale University |
| issn | 1734-154X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Acta Biochimica Polonica |
| spelling | doaj-art-7ee1058b8569426cbbe2d0322fccf7442025-08-20T03:44:06ZengFrontiers Media S.A.Acta Biochimica Polonica1734-154X2025-08-017210.3389/abp.2025.1464214642Multi-modal comparison of murine and human incisal dentin-enamel junctionsMichael Truhlar0Sobhan Katebifar1Bradley Rosenberg2Roland Kroger3Alix C. Deymier4School of Dental Medicine, UConn Health Center, Farmington, CT, United StatesDepartment of Biomedical Engineering, UConn Health Center, Farmington, CT, United StatesSchool of Dental Medicine, UConn Health Center, Farmington, CT, United StatesDepartment of Physics, University of York, York, United KingdomDepartment of Biomedical Engineering, UConn Health Center, Farmington, CT, United StatesHuman and mouse incisors are both primarily composed of dentin and enamel, which meet at an interface called the dentin-enamel junction (DEJ). However, incisors in the two species have very different growth patterns, structures, and loading requirements. Since the DEJ is responsible for minimizing cracking at this at-risk interface between mechanically dissimilar dentin and enamel, its structure is expected to be significantly different between humans and mice. Here, structural and compositional gradients across human and murine incisors DEJs were measured via microcomputed tomography and Raman spectroscopy. Density gradients across the DEJ were significantly larger in humans compared to murine teeth, likely due to the larger size of the mantle dentin. Multiple gradients in mineral content and crystallinity were found at the murine DEJ, while the human DEJ only exhibited gradients in mineral content. Models predicting the modulus across the DEJ according to compositional results show that mineral crystallinity is critical in regulating gradients in tissue stiffness across the murine DEJ. Together, these results show the multiple ways in which the DEJ can adapt to variations in the loading environment.https://www.frontierspartnerships.org/articles/10.3389/abp.2025.14642/fulldental Morphologydentinenamelmechanical propertiesmicro-computed tomography |
| spellingShingle | Michael Truhlar Sobhan Katebifar Bradley Rosenberg Roland Kroger Alix C. Deymier Multi-modal comparison of murine and human incisal dentin-enamel junctions Acta Biochimica Polonica dental Morphology dentin enamel mechanical properties micro-computed tomography |
| title | Multi-modal comparison of murine and human incisal dentin-enamel junctions |
| title_full | Multi-modal comparison of murine and human incisal dentin-enamel junctions |
| title_fullStr | Multi-modal comparison of murine and human incisal dentin-enamel junctions |
| title_full_unstemmed | Multi-modal comparison of murine and human incisal dentin-enamel junctions |
| title_short | Multi-modal comparison of murine and human incisal dentin-enamel junctions |
| title_sort | multi modal comparison of murine and human incisal dentin enamel junctions |
| topic | dental Morphology dentin enamel mechanical properties micro-computed tomography |
| url | https://www.frontierspartnerships.org/articles/10.3389/abp.2025.14642/full |
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