Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetes
Oxidative stress has been hypothesized to play a crucial role in the complications of type 2 diabetes (T2D). Hyperglycaemia-mediated increases in free radicals have a deleterious effect on cellular compartments and nucleic acids, leading to imbalances between free radicals and antioxidant enzymes. T...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | All Life |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2391298 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593479917633536 |
|---|---|
| author | Samar Sultan Meshari Alharbi Nuha Alrayes Nehad Makki Amani Alhozali Reham Abdulnoor Aloof Alsawat Haitham Khalil Noha Jawi Ahmed Makeen |
| author_facet | Samar Sultan Meshari Alharbi Nuha Alrayes Nehad Makki Amani Alhozali Reham Abdulnoor Aloof Alsawat Haitham Khalil Noha Jawi Ahmed Makeen |
| author_sort | Samar Sultan |
| collection | DOAJ |
| description | Oxidative stress has been hypothesized to play a crucial role in the complications of type 2 diabetes (T2D). Hyperglycaemia-mediated increases in free radicals have a deleterious effect on cellular compartments and nucleic acids, leading to imbalances between free radicals and antioxidant enzymes. This case–control study comprised two groups with 100 participants (50 T2D and 50 DN patients) and aimed to examine the association between single-nucleotide polymorphisms (SNPs) in the genes encoding nicotinamide adenine dinucleotide phosphate oxidase (NOX), glutathione S-transferase P (GSTP1), and glutathione peroxidase 1(GPX1) and diabetic nephropathy (DN) risk in patients with T2D. An SNP genotyping assay was performed using TaqMan assay and real-time PCR to identify the SNPs (NOX rs4673, GSTP rs1695, GPX1 rs1050450). The Sanger method was used to validate our findings. Fisher chi-square analyses revealed no significant differences in these genes when comparing T2D patients with and without DN. Our findings suggest no association between the rs4673, rs1695, and rs1050450 SNPs and DN in Saudi patients with T2D. |
| format | Article |
| id | doaj-art-7edebd146da44a03ae6551f91b381f84 |
| institution | Kabale University |
| issn | 2689-5307 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | All Life |
| spelling | doaj-art-7edebd146da44a03ae6551f91b381f842025-01-20T14:38:00ZengTaylor & Francis GroupAll Life2689-53072024-12-01170110.1080/26895293.2024.23912982391298Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetesSamar Sultan0Meshari Alharbi1Nuha Alrayes2Nehad Makki3Amani Alhozali4Reham Abdulnoor5Aloof Alsawat6Haitham Khalil7Noha Jawi8Ahmed Makeen9King Abdulaziz UniversityKing Abdulaziz UniversityKing Abdulaziz UniversityCollege of Medicine, King Abdulaziz UniversityCollege of Medicine, King Abdulaziz UniversityCollege of Medicine, King Abdulaziz UniversityMakkah Healthcare Cluster, Maternity and Children’s HospitalKing Saud bin Abdulaziz University for Health Sciences (KSAU-HS)National Guard HospitalDepartment of Nephrology, Jeddah, Saudi ArabiaOxidative stress has been hypothesized to play a crucial role in the complications of type 2 diabetes (T2D). Hyperglycaemia-mediated increases in free radicals have a deleterious effect on cellular compartments and nucleic acids, leading to imbalances between free radicals and antioxidant enzymes. This case–control study comprised two groups with 100 participants (50 T2D and 50 DN patients) and aimed to examine the association between single-nucleotide polymorphisms (SNPs) in the genes encoding nicotinamide adenine dinucleotide phosphate oxidase (NOX), glutathione S-transferase P (GSTP1), and glutathione peroxidase 1(GPX1) and diabetic nephropathy (DN) risk in patients with T2D. An SNP genotyping assay was performed using TaqMan assay and real-time PCR to identify the SNPs (NOX rs4673, GSTP rs1695, GPX1 rs1050450). The Sanger method was used to validate our findings. Fisher chi-square analyses revealed no significant differences in these genes when comparing T2D patients with and without DN. Our findings suggest no association between the rs4673, rs1695, and rs1050450 SNPs and DN in Saudi patients with T2D.http://dx.doi.org/10.1080/26895293.2024.2391298type 2 diabetessingle-nucleotide polymorphismsgenotypingdiabetic nephropathyoxidative stressantioxidants |
| spellingShingle | Samar Sultan Meshari Alharbi Nuha Alrayes Nehad Makki Amani Alhozali Reham Abdulnoor Aloof Alsawat Haitham Khalil Noha Jawi Ahmed Makeen Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetes All Life type 2 diabetes single-nucleotide polymorphisms genotyping diabetic nephropathy oxidative stress antioxidants |
| title | Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetes |
| title_full | Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetes |
| title_fullStr | Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetes |
| title_full_unstemmed | Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetes |
| title_short | Gene Polymorphisms of the antioxidant enzymes NOX, GSTP, and GPX and diabetic nephropathy risk in Saudi patients with type 2 diabetes |
| title_sort | gene polymorphisms of the antioxidant enzymes nox gstp and gpx and diabetic nephropathy risk in saudi patients with type 2 diabetes |
| topic | type 2 diabetes single-nucleotide polymorphisms genotyping diabetic nephropathy oxidative stress antioxidants |
| url | http://dx.doi.org/10.1080/26895293.2024.2391298 |
| work_keys_str_mv | AT samarsultan genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT mesharialharbi genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT nuhaalrayes genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT nehadmakki genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT amanialhozali genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT rehamabdulnoor genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT aloofalsawat genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT haithamkhalil genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT nohajawi genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes AT ahmedmakeen genepolymorphismsoftheantioxidantenzymesnoxgstpandgpxanddiabeticnephropathyriskinsaudipatientswithtype2diabetes |