Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites
Heterochiral oligodeoxynucleotides based on the thrombin binding aptamer sequence, namely, 5′gg3′-3′TT5′-5′ggtgtgg3′-3′TT5′-5′gg3′ (H1), 5′gg3′-3′TT5′-5′gg3′-3′TGT5′-5′gg3′-3′TT5′-5′gg3′ (H2), and 5′gGTTGgtgtgGTTGg3′ (H3), where lower case letters indicate L-residues, have been investigated in their...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/473051 |
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| author | Antonella Virgilio Luciano Mayol Michela Varra Aldo Galeone Veronica Esposito |
| author_facet | Antonella Virgilio Luciano Mayol Michela Varra Aldo Galeone Veronica Esposito |
| author_sort | Antonella Virgilio |
| collection | DOAJ |
| description | Heterochiral oligodeoxynucleotides based on the thrombin binding aptamer sequence, namely, 5′gg3′-3′TT5′-5′ggtgtgg3′-3′TT5′-5′gg3′ (H1), 5′gg3′-3′TT5′-5′gg3′-3′TGT5′-5′gg3′-3′TT5′-5′gg3′ (H2), and 5′gGTTGgtgtgGTTGg3′ (H3), where lower case letters indicate L-residues, have been investigated in their ability to fold in G-quadruplex structures through a combination of gel electrophoresis, circular dichroism, and UV spectroscopy techniques. In H1 and H2 inversions of polarity sites have been introduced to control the strand direction in the loop regions. Collected data suggest that all modified sequences are able to fold in chair-like G-quadruplexes mimicking the original TBA structure. |
| format | Article |
| id | doaj-art-7ede7e25476f4101bac84499484bc5b9 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-7ede7e25476f4101bac84499484bc5b92025-02-03T06:42:06ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/473051473051Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity SitesAntonella Virgilio0Luciano Mayol1Michela Varra2Aldo Galeone3Veronica Esposito4Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49-80131 Naples, ItalyHeterochiral oligodeoxynucleotides based on the thrombin binding aptamer sequence, namely, 5′gg3′-3′TT5′-5′ggtgtgg3′-3′TT5′-5′gg3′ (H1), 5′gg3′-3′TT5′-5′gg3′-3′TGT5′-5′gg3′-3′TT5′-5′gg3′ (H2), and 5′gGTTGgtgtgGTTGg3′ (H3), where lower case letters indicate L-residues, have been investigated in their ability to fold in G-quadruplex structures through a combination of gel electrophoresis, circular dichroism, and UV spectroscopy techniques. In H1 and H2 inversions of polarity sites have been introduced to control the strand direction in the loop regions. Collected data suggest that all modified sequences are able to fold in chair-like G-quadruplexes mimicking the original TBA structure.http://dx.doi.org/10.1155/2015/473051 |
| spellingShingle | Antonella Virgilio Luciano Mayol Michela Varra Aldo Galeone Veronica Esposito Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites Journal of Chemistry |
| title | Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites |
| title_full | Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites |
| title_fullStr | Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites |
| title_full_unstemmed | Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites |
| title_short | Unusual Chair-Like G-Quadruplex Structures: Heterochiral TBA Analogues Containing Inversion of Polarity Sites |
| title_sort | unusual chair like g quadruplex structures heterochiral tba analogues containing inversion of polarity sites |
| url | http://dx.doi.org/10.1155/2015/473051 |
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