Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes
Essential oils from the dried spikes of Nepeta tenuifolia (Benth) are obtained by steam distillation. Pulegone was identified as the main component in the spikes of N. tenuifolia through analysis, with greater than 85% purity obtained in this study. The essential oils are extremely active against al...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/8347403 |
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| author | Yi-Hsuan Lee Chao-Min Wang Po-Yu Liu Ching-Chang Cheng Zong-Yen Wu Shu-Ying Tseng Kwong-Chung Tung |
| author_facet | Yi-Hsuan Lee Chao-Min Wang Po-Yu Liu Ching-Chang Cheng Zong-Yen Wu Shu-Ying Tseng Kwong-Chung Tung |
| author_sort | Yi-Hsuan Lee |
| collection | DOAJ |
| description | Essential oils from the dried spikes of Nepeta tenuifolia (Benth) are obtained by steam distillation. Pulegone was identified as the main component in the spikes of N. tenuifolia through analysis, with greater than 85% purity obtained in this study. The essential oils are extremely active against all Gram-positive and some Gram-negative reference bacteria, particularly Salmonella enterica, Citrobacter freundii, and Escherichia coli. The minimum inhibitory concentration was found to be between 0.08 and 0.78% (against S. enterica), 0.39 and 0.78% (against C. freundii), and 0.097 and 0.39% (against E. coli), whereas the minimum bactericidal concentration varied in range from 0.097% to 1.04%. In general, the essential oils show a strong inhibitory action against all tested reference strains and clinical isolates. However, the antibacterial activity of EOs against both Pseudomonas aeruginosa reference strains and clinical isolates was relatively lower than other Gram-negative pathogens. The essential oils of N. tenuifolia also displayed bactericidal activities (MBC/MIC < 4) in this study. These findings reflect the bactericidal activity of the essential oils against a wide range of multidrug-resistant clinical pathogens in an in vitro study. In addition, we propose the fragmentation pathways of pulegone and its derivatives by LC-ESI-MS/MS in this study. |
| format | Article |
| id | doaj-art-7ec94ff6302f4610b4bb515e9282a187 |
| institution | OA Journals |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-7ec94ff6302f4610b4bb515e9282a1872025-08-20T02:09:00ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932018-01-01201810.1155/2018/83474038347403Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic MicrobesYi-Hsuan Lee0Chao-Min Wang1Po-Yu Liu2Ching-Chang Cheng3Zong-Yen Wu4Shu-Ying Tseng5Kwong-Chung Tung6Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, TaiwanResearch Center for Biodiversity, China Medical University, Taichung, TaiwanDepartment of Internal Medicine, Taichung Veterans General Hospital, Taichung, TaiwanLaboratory Animal Service Center, Office of Research and Development, China Medical University, Taichung 40402, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, TaiwanEssential oils from the dried spikes of Nepeta tenuifolia (Benth) are obtained by steam distillation. Pulegone was identified as the main component in the spikes of N. tenuifolia through analysis, with greater than 85% purity obtained in this study. The essential oils are extremely active against all Gram-positive and some Gram-negative reference bacteria, particularly Salmonella enterica, Citrobacter freundii, and Escherichia coli. The minimum inhibitory concentration was found to be between 0.08 and 0.78% (against S. enterica), 0.39 and 0.78% (against C. freundii), and 0.097 and 0.39% (against E. coli), whereas the minimum bactericidal concentration varied in range from 0.097% to 1.04%. In general, the essential oils show a strong inhibitory action against all tested reference strains and clinical isolates. However, the antibacterial activity of EOs against both Pseudomonas aeruginosa reference strains and clinical isolates was relatively lower than other Gram-negative pathogens. The essential oils of N. tenuifolia also displayed bactericidal activities (MBC/MIC < 4) in this study. These findings reflect the bactericidal activity of the essential oils against a wide range of multidrug-resistant clinical pathogens in an in vitro study. In addition, we propose the fragmentation pathways of pulegone and its derivatives by LC-ESI-MS/MS in this study.http://dx.doi.org/10.1155/2018/8347403 |
| spellingShingle | Yi-Hsuan Lee Chao-Min Wang Po-Yu Liu Ching-Chang Cheng Zong-Yen Wu Shu-Ying Tseng Kwong-Chung Tung Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes |
| title_full | Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes |
| title_fullStr | Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes |
| title_full_unstemmed | Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes |
| title_short | Volatile Oils of Nepeta tenuifolia (Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes |
| title_sort | volatile oils of nepeta tenuifolia jing jie as an alternative medicine against multidrug resistant pathogenic microbes |
| url | http://dx.doi.org/10.1155/2018/8347403 |
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