The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism
Objective To investigate the effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism. Methods Human cholangiocarcinoma cell line (QBC939 cells) were divided into QBC939 group (group A), QBC939+well-d...
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Editorial Office of Journal of Precision Medicine
2025-08-01
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| Series: | 精准医学杂志 |
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| Online Access: | https://jpmed.qdu.edu.cn/fileup/2096-529X/PDF/1754471523532-1188741439.pdf |
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| author | CHEN Menshou, FENG Yujie |
| author_facet | CHEN Menshou, FENG Yujie |
| author_sort | CHEN Menshou, FENG Yujie |
| collection | DOAJ |
| description | Objective To investigate the effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism. Methods Human cholangiocarcinoma cell line (QBC939 cells) were divided into QBC939 group (group A), QBC939+well-differentiated pheochromocytoma cell line (PC12) group (group B), and QBC939+PC12+bupivacaine group (group C). The cells in group A were cultured in the QBC939 cell-specific medium for 48 h, those in group B were co-cultured with well-differentiated PC12 cells for 48 h in addition to the treatment in group A to establish the in vitro model of perineural invasion of cholangiocarcinoma, and those in group C were cultured with bupivacaine for 48 h in addition to the treatment in group B. CCK-8 assay, EDU staining, cell scratch assay, and Transwell assay were used to measure cell viability and assess the proliferation, migration, and invasion abilities of the three groups of cells. Well-diffe-rentiated PC12 cells were divided into PC12 group (group D) and PC12+bupivacaine group (group E), and the cells in group D were cultured in the PC12 cell-specific medium for 48 h, while those in group E were cultured with bupivacaine for 48 h in addition to the treatment in group D. Immunofluorescence staining assay was used to measure the length of cell axons, and Western blotting was used to measure the relative expression level of neuropilin-1 (NRP-1) in the two groups. Results CCK-8 assay, EDU staining, cell scratch assay, and Transwell assay showed that compared with B, group C had significantly lower cell viability and proliferation, migration, and invasion abilities of QBC939 cells (F=34.39-149.20,q=9.47-12.95,P<0.05). Immunofluorescent staining and Western blotting showed that compared with group D, group E had a significantly shorter mean length of cell axons and a significantly lower relative protein expression level of NRP-1 (t=11.75,3.09,P<0.05). Conclusion Bupivacaine may inhibit the malignant biological behaviors of cholangiocarcinoma cells such as proliferation, migration, and invasion by inhibiting the axonal growth of neural cells and the expression of NRP-1 in an in vitro model of perineural invasion of cholangiocarcinoma. |
| format | Article |
| id | doaj-art-7ec28b829a524e7296ffe3fea1667378 |
| institution | Kabale University |
| issn | 2096-529X |
| language | zho |
| publishDate | 2025-08-01 |
| publisher | Editorial Office of Journal of Precision Medicine |
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| series | 精准医学杂志 |
| spelling | doaj-art-7ec28b829a524e7296ffe3fea16673782025-08-20T04:00:28ZzhoEditorial Office of Journal of Precision Medicine精准医学杂志2096-529X2025-08-0140430330810.13362/j.jpmed.202540046The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanismCHEN Menshou, FENG Yujie0Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266003, ChinaObjective To investigate the effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism. Methods Human cholangiocarcinoma cell line (QBC939 cells) were divided into QBC939 group (group A), QBC939+well-differentiated pheochromocytoma cell line (PC12) group (group B), and QBC939+PC12+bupivacaine group (group C). The cells in group A were cultured in the QBC939 cell-specific medium for 48 h, those in group B were co-cultured with well-differentiated PC12 cells for 48 h in addition to the treatment in group A to establish the in vitro model of perineural invasion of cholangiocarcinoma, and those in group C were cultured with bupivacaine for 48 h in addition to the treatment in group B. CCK-8 assay, EDU staining, cell scratch assay, and Transwell assay were used to measure cell viability and assess the proliferation, migration, and invasion abilities of the three groups of cells. Well-diffe-rentiated PC12 cells were divided into PC12 group (group D) and PC12+bupivacaine group (group E), and the cells in group D were cultured in the PC12 cell-specific medium for 48 h, while those in group E were cultured with bupivacaine for 48 h in addition to the treatment in group D. Immunofluorescence staining assay was used to measure the length of cell axons, and Western blotting was used to measure the relative expression level of neuropilin-1 (NRP-1) in the two groups. Results CCK-8 assay, EDU staining, cell scratch assay, and Transwell assay showed that compared with B, group C had significantly lower cell viability and proliferation, migration, and invasion abilities of QBC939 cells (F=34.39-149.20,q=9.47-12.95,P<0.05). Immunofluorescent staining and Western blotting showed that compared with group D, group E had a significantly shorter mean length of cell axons and a significantly lower relative protein expression level of NRP-1 (t=11.75,3.09,P<0.05). Conclusion Bupivacaine may inhibit the malignant biological behaviors of cholangiocarcinoma cells such as proliferation, migration, and invasion by inhibiting the axonal growth of neural cells and the expression of NRP-1 in an in vitro model of perineural invasion of cholangiocarcinoma.https://jpmed.qdu.edu.cn/fileup/2096-529X/PDF/1754471523532-1188741439.pdfbile duct neoplasms|bupivacaine|neuropilin-1|neurons|axons|neoplasm invasiveness|cell proliferation|cell movement |
| spellingShingle | CHEN Menshou, FENG Yujie The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism 精准医学杂志 bile duct neoplasms|bupivacaine|neuropilin-1|neurons|axons|neoplasm invasiveness|cell proliferation|cell movement |
| title | The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism |
| title_full | The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism |
| title_fullStr | The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism |
| title_full_unstemmed | The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism |
| title_short | The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism |
| title_sort | effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism |
| topic | bile duct neoplasms|bupivacaine|neuropilin-1|neurons|axons|neoplasm invasiveness|cell proliferation|cell movement |
| url | https://jpmed.qdu.edu.cn/fileup/2096-529X/PDF/1754471523532-1188741439.pdf |
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