In vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment

Abstract The cervix is a vital component of pregnancy: it provides a protective barrier during the antepartum period and undergoes drastic remodeling to facilitate parturition. There is a wealth of research that studies cervical remodeling during late pregnancy and labor, but few studies investigate...

Full description

Saved in:
Bibliographic Details
Main Authors: Emily E. Spurlin, Katy Belcher, Folaoluwashewa Shofu, Jennifer A. E. Esteves, Patricia T. Jimenez, Christine M. O’Brien
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:npj Women's Health
Online Access:https://doi.org/10.1038/s44294-025-00083-x
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850224332562759680
author Emily E. Spurlin
Katy Belcher
Folaoluwashewa Shofu
Jennifer A. E. Esteves
Patricia T. Jimenez
Christine M. O’Brien
author_facet Emily E. Spurlin
Katy Belcher
Folaoluwashewa Shofu
Jennifer A. E. Esteves
Patricia T. Jimenez
Christine M. O’Brien
author_sort Emily E. Spurlin
collection DOAJ
description Abstract The cervix is a vital component of pregnancy: it provides a protective barrier during the antepartum period and undergoes drastic remodeling to facilitate parturition. There is a wealth of research that studies cervical remodeling during late pregnancy and labor, but few studies investigate the cervix during the earliest weeks of gestation. This study focuses on biochemical changes that occur between the time just prior to conception and 6–8 weeks gestation by enrolling patients undergoing intrauterine insemination. In vivo Raman spectroscopy was utilized as a nondestructive tool to identify early biochemical changes within the cervix. The study found the early cervical remodeling included increased vascularity, decreased structural proteins, and decreased trivalent collagen cross-linking. This is the first study demonstrating the feasibility of using in vivo Raman spectroscopy to measure biochemical changes upon the transition to early pregnancy and indicates that cervical remodeling begins in the earliest stages of pregnancy.
format Article
id doaj-art-7ec0aaa99eb946ee8ff6d526505d039b
institution OA Journals
issn 2948-1716
language English
publishDate 2025-06-01
publisher Nature Portfolio
record_format Article
series npj Women's Health
spelling doaj-art-7ec0aaa99eb946ee8ff6d526505d039b2025-08-20T02:05:39ZengNature Portfolionpj Women's Health2948-17162025-06-01311710.1038/s44294-025-00083-xIn vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishmentEmily E. Spurlin0Katy Belcher1Folaoluwashewa Shofu2Jennifer A. E. Esteves3Patricia T. Jimenez4Christine M. O’Brien5Department of Obstetrics & Gynecology, Division of Reproductive Endocrinology and Infertility, Washington University in St. LouisDepartment of Biomedical Engineering, Washington University in St. LouisDepartment of Biomedical Engineering, Washington University in St. LouisDepartment of Biomedical Engineering, Washington University in St. LouisDepartment of Obstetrics & Gynecology, Division of Reproductive Endocrinology and Infertility, Washington University in St. LouisDepartment of Biomedical Engineering, Washington University in St. LouisAbstract The cervix is a vital component of pregnancy: it provides a protective barrier during the antepartum period and undergoes drastic remodeling to facilitate parturition. There is a wealth of research that studies cervical remodeling during late pregnancy and labor, but few studies investigate the cervix during the earliest weeks of gestation. This study focuses on biochemical changes that occur between the time just prior to conception and 6–8 weeks gestation by enrolling patients undergoing intrauterine insemination. In vivo Raman spectroscopy was utilized as a nondestructive tool to identify early biochemical changes within the cervix. The study found the early cervical remodeling included increased vascularity, decreased structural proteins, and decreased trivalent collagen cross-linking. This is the first study demonstrating the feasibility of using in vivo Raman spectroscopy to measure biochemical changes upon the transition to early pregnancy and indicates that cervical remodeling begins in the earliest stages of pregnancy.https://doi.org/10.1038/s44294-025-00083-x
spellingShingle Emily E. Spurlin
Katy Belcher
Folaoluwashewa Shofu
Jennifer A. E. Esteves
Patricia T. Jimenez
Christine M. O’Brien
In vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment
npj Women's Health
title In vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment
title_full In vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment
title_fullStr In vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment
title_full_unstemmed In vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment
title_short In vivo Raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment
title_sort in vivo raman spectroscopy reveals biochemical changes in the human cervix with pregnancy establishment
url https://doi.org/10.1038/s44294-025-00083-x
work_keys_str_mv AT emilyespurlin invivoramanspectroscopyrevealsbiochemicalchangesinthehumancervixwithpregnancyestablishment
AT katybelcher invivoramanspectroscopyrevealsbiochemicalchangesinthehumancervixwithpregnancyestablishment
AT folaoluwashewashofu invivoramanspectroscopyrevealsbiochemicalchangesinthehumancervixwithpregnancyestablishment
AT jenniferaeesteves invivoramanspectroscopyrevealsbiochemicalchangesinthehumancervixwithpregnancyestablishment
AT patriciatjimenez invivoramanspectroscopyrevealsbiochemicalchangesinthehumancervixwithpregnancyestablishment
AT christinemobrien invivoramanspectroscopyrevealsbiochemicalchangesinthehumancervixwithpregnancyestablishment