The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis.
The rapid evolution of toxin resistance in animals has important consequences for the ecology of species and our economy. Pesticide resistance in insects has been a subject of intensive study; however, very little is known about how Drosophila species became resistant to natural toxins with ecologic...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093489&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850026486791143424 |
|---|---|
| author | Chelsea L Mitchell Michael C Saul Liang Lei Hairong Wei Thomas Werner |
| author_facet | Chelsea L Mitchell Michael C Saul Liang Lei Hairong Wei Thomas Werner |
| author_sort | Chelsea L Mitchell |
| collection | DOAJ |
| description | The rapid evolution of toxin resistance in animals has important consequences for the ecology of species and our economy. Pesticide resistance in insects has been a subject of intensive study; however, very little is known about how Drosophila species became resistant to natural toxins with ecological relevance, such as α-amanitin that is produced in deadly poisonous mushrooms. Here we performed a microarray study to elucidate the genes, chromosomal loci, molecular functions, biological processes, and cellular components that contribute to the α-amanitin resistance phenotype in Drosophila melanogaster. We suggest that toxin entry blockage through the cuticle, phase I and II detoxification, sequestration in lipid particles, and proteolytic cleavage of α-amanitin contribute in concert to this quantitative trait. We speculate that the resistance to mushroom toxins in D. melanogaster and perhaps in mycophagous Drosophila species has evolved as cross-resistance to pesticides, other xenobiotic substances, or environmental stress factors. |
| format | Article |
| id | doaj-art-7eb6c5ed5eb146e08874b3103da66779 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7eb6c5ed5eb146e08874b3103da667792025-08-20T03:00:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9348910.1371/journal.pone.0093489The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis.Chelsea L MitchellMichael C SaulLiang LeiHairong WeiThomas WernerThe rapid evolution of toxin resistance in animals has important consequences for the ecology of species and our economy. Pesticide resistance in insects has been a subject of intensive study; however, very little is known about how Drosophila species became resistant to natural toxins with ecological relevance, such as α-amanitin that is produced in deadly poisonous mushrooms. Here we performed a microarray study to elucidate the genes, chromosomal loci, molecular functions, biological processes, and cellular components that contribute to the α-amanitin resistance phenotype in Drosophila melanogaster. We suggest that toxin entry blockage through the cuticle, phase I and II detoxification, sequestration in lipid particles, and proteolytic cleavage of α-amanitin contribute in concert to this quantitative trait. We speculate that the resistance to mushroom toxins in D. melanogaster and perhaps in mycophagous Drosophila species has evolved as cross-resistance to pesticides, other xenobiotic substances, or environmental stress factors.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093489&type=printable |
| spellingShingle | Chelsea L Mitchell Michael C Saul Liang Lei Hairong Wei Thomas Werner The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis. PLoS ONE |
| title | The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis. |
| title_full | The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis. |
| title_fullStr | The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis. |
| title_full_unstemmed | The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis. |
| title_short | The mechanisms underlying α-amanitin resistance in Drosophila melanogaster: a microarray analysis. |
| title_sort | mechanisms underlying α amanitin resistance in drosophila melanogaster a microarray analysis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093489&type=printable |
| work_keys_str_mv | AT chelsealmitchell themechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT michaelcsaul themechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT lianglei themechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT hairongwei themechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT thomaswerner themechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT chelsealmitchell mechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT michaelcsaul mechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT lianglei mechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT hairongwei mechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis AT thomaswerner mechanismsunderlyingaamanitinresistanceindrosophilamelanogasteramicroarrayanalysis |