Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart
Aim. To study the effect of simultaneous administration of creatine phosphate immediately before ischemia on cardiodynamic parameters and biomarkers of oxidative stress in the coronary venous blood flow during retrograde perfusion in an isolated rat heart.Materials and methods. 20 Wistar albino rats...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
2021-10-01
|
| Series: | Сеченовский вестник |
| Subjects: | |
| Online Access: | https://www.sechenovmedj.com/jour/article/view/453 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850050724069638144 |
|---|---|
| author | L. A. Gadjieva S. B. Bolevich V. Jakovlevich I. A. Omarov H. A. Ordashev M. K. Kartashova |
| author_facet | L. A. Gadjieva S. B. Bolevich V. Jakovlevich I. A. Omarov H. A. Ordashev M. K. Kartashova |
| author_sort | L. A. Gadjieva |
| collection | DOAJ |
| description | Aim. To study the effect of simultaneous administration of creatine phosphate immediately before ischemia on cardiodynamic parameters and biomarkers of oxidative stress in the coronary venous blood flow during retrograde perfusion in an isolated rat heart.Materials and methods. 20 Wistar albino rats were divided into 2 groups: group 1 (control) and group 2 (experimental), 10 rats per group. Cannulation and retrograde perfusion of aorta of an isolated rat heart with Krebs–Henseleit buffered solution by Landendorff was performed. Both groups underwent ischemia-reperfusion injury, which included global ischemia for 20 minutes followed by reperfusion for 30 minutes. The group 2 (experimental) was preconditioned with creatine phosphate at a dose of 0.2 mmol/l for 5 min before ischemia. We registered cardiodynamic parameters and indicators of oxidative stress at the point of stabilization, at the 1st and 30th minutes of reperfusion.Results. With the impact of creatine phosphate at the 30th minute of reperfusion in the group 2 in comparison with group 1, there was found an increase in the maximum and minimum speed of pressure elevation in the left ventricle (1.7 and 1.9 times, respectively), and of systolic and diastolic pressure in the left ventricle (1.5 and 1.6 times, respectively). Biomarkers of oxidative stress (lipid peroxidation index, nitrites, superoxide anion radical and hydrogen peroxide) were also statistically significantly lower in the group 2 after the 1st minute of reperfusion (by 1.2 times, by 1.4 times, by 2.8 times and 1.9 times, respectively), and after the 30th minute (1.3 times, 2.1 times, 1.9 times and 2.1 times, respectively).Conclusion. The administration of creatine phosphate into the coronary flow 5 minutes before the onset of ischemia has a protective effect on myocardial contractility. Reduction of oxidative stress and damage can be considered as a protective effect of creatine phosphate. |
| format | Article |
| id | doaj-art-7ea491977f3b4f13b7e5e375b6d3cf20 |
| institution | DOAJ |
| issn | 2218-7332 2658-3348 |
| language | Russian |
| publishDate | 2021-10-01 |
| publisher | Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University) |
| record_format | Article |
| series | Сеченовский вестник |
| spelling | doaj-art-7ea491977f3b4f13b7e5e375b6d3cf202025-08-20T02:53:22ZrusFederal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)Сеченовский вестник2218-73322658-33482021-10-0113110.47093/2218-7332.2022.13.1.24-33222Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heartL. A. Gadjieva0S. B. Bolevich1V. Jakovlevich2I. A. Omarov3H. A. Ordashev4M. K. Kartashova5Medical and Sanitary Unit of the Ministry of Internal Affairs of Russia in the Republic of DagestanSechenov First Moscow State Medical University (Sechenov University)Sechenov First Moscow State Medical University (Sechenov University); University of KragujevacHealth-Related Center of Ministry of External Affairs of Russian FederationDagestan State Medical UnivesitySechenov First Moscow State Medical University (Sechenov University)Aim. To study the effect of simultaneous administration of creatine phosphate immediately before ischemia on cardiodynamic parameters and biomarkers of oxidative stress in the coronary venous blood flow during retrograde perfusion in an isolated rat heart.Materials and methods. 20 Wistar albino rats were divided into 2 groups: group 1 (control) and group 2 (experimental), 10 rats per group. Cannulation and retrograde perfusion of aorta of an isolated rat heart with Krebs–Henseleit buffered solution by Landendorff was performed. Both groups underwent ischemia-reperfusion injury, which included global ischemia for 20 minutes followed by reperfusion for 30 minutes. The group 2 (experimental) was preconditioned with creatine phosphate at a dose of 0.2 mmol/l for 5 min before ischemia. We registered cardiodynamic parameters and indicators of oxidative stress at the point of stabilization, at the 1st and 30th minutes of reperfusion.Results. With the impact of creatine phosphate at the 30th minute of reperfusion in the group 2 in comparison with group 1, there was found an increase in the maximum and minimum speed of pressure elevation in the left ventricle (1.7 and 1.9 times, respectively), and of systolic and diastolic pressure in the left ventricle (1.5 and 1.6 times, respectively). Biomarkers of oxidative stress (lipid peroxidation index, nitrites, superoxide anion radical and hydrogen peroxide) were also statistically significantly lower in the group 2 after the 1st minute of reperfusion (by 1.2 times, by 1.4 times, by 2.8 times and 1.9 times, respectively), and after the 30th minute (1.3 times, 2.1 times, 1.9 times and 2.1 times, respectively).Conclusion. The administration of creatine phosphate into the coronary flow 5 minutes before the onset of ischemia has a protective effect on myocardial contractility. Reduction of oxidative stress and damage can be considered as a protective effect of creatine phosphate.https://www.sechenovmedj.com/jour/article/view/453creatine phosphatepreconditioningischemia/reperfusion injuryoxidative stresscardiodynamicsisolated rat heartlangendorff technique |
| spellingShingle | L. A. Gadjieva S. B. Bolevich V. Jakovlevich I. A. Omarov H. A. Ordashev M. K. Kartashova Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart Сеченовский вестник creatine phosphate preconditioning ischemia/reperfusion injury oxidative stress cardiodynamics isolated rat heart langendorff technique |
| title | Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart |
| title_full | Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart |
| title_fullStr | Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart |
| title_full_unstemmed | Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart |
| title_short | Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart |
| title_sort | creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart |
| topic | creatine phosphate preconditioning ischemia/reperfusion injury oxidative stress cardiodynamics isolated rat heart langendorff technique |
| url | https://www.sechenovmedj.com/jour/article/view/453 |
| work_keys_str_mv | AT lagadjieva creatinephosphatepreconditioningreducesischemiareperfusioninjuryinisolatedratheart AT sbbolevich creatinephosphatepreconditioningreducesischemiareperfusioninjuryinisolatedratheart AT vjakovlevich creatinephosphatepreconditioningreducesischemiareperfusioninjuryinisolatedratheart AT iaomarov creatinephosphatepreconditioningreducesischemiareperfusioninjuryinisolatedratheart AT haordashev creatinephosphatepreconditioningreducesischemiareperfusioninjuryinisolatedratheart AT mkkartashova creatinephosphatepreconditioningreducesischemiareperfusioninjuryinisolatedratheart |