In vivo self-renewal and expansion of quiescent stem cells from a non-human primate
Abstract The development of non-human primate models is essential for the fields of developmental and regenerative biology because those models will more closely approximate human biology than do murine models. Based on single cell RNAseq and fluorescence-activated cell sorting, we report the identi...
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Nature Portfolio
2025-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-58897-x |
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| author | Jengmin Kang Abhijnya Kanugovi M. Pilar J. Stella Zofija Frimand Jean Farup Andoni Urtasun Shixuan Liu Anne-Sofie Clausen Heather Ishak Summer Bui Soochi Kim Camille Ezran Olga Botvinnik Ermelinda Porpiglia Mark A. Krasnow Antoine de Morree Thomas A. Rando |
| author_facet | Jengmin Kang Abhijnya Kanugovi M. Pilar J. Stella Zofija Frimand Jean Farup Andoni Urtasun Shixuan Liu Anne-Sofie Clausen Heather Ishak Summer Bui Soochi Kim Camille Ezran Olga Botvinnik Ermelinda Porpiglia Mark A. Krasnow Antoine de Morree Thomas A. Rando |
| author_sort | Jengmin Kang |
| collection | DOAJ |
| description | Abstract The development of non-human primate models is essential for the fields of developmental and regenerative biology because those models will more closely approximate human biology than do murine models. Based on single cell RNAseq and fluorescence-activated cell sorting, we report the identification and functional characterization of two quiescent stem cell populations (skeletal muscle stem cells (MuSCs) and mesenchymal stem cells termed fibro-adipogenic progenitors (FAPs)) in the non-human primate Microcebus murinus (the gray mouse lemur). We demonstrate in vivo proliferation, differentiation, and self-renewal of both MuSCs and FAPs. By combining cell phenotyping with cross-species molecular profiling and pharmacological interventions, we show that mouse lemur MuSCs and FAPs are more similar to human than to mouse counterparts. We identify unexpected gene targets involved in regulating primate MuSC proliferation and primate FAP adipogenic differentiation. Moreover, we find that the cellular composition of mouse lemur muscle better models human muscle than does macaque (Macaca fascicularis) muscle. Finally, we note that our approach presents as a generalizable pipeline for the identification, isolation, and characterization of stem cell populations in new animal models. |
| format | Article |
| id | doaj-art-7ea3d04e2ef34a3e8e0665f168d8984a |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-7ea3d04e2ef34a3e8e0665f168d8984a2025-08-20T02:37:58ZengNature PortfolioNature Communications2041-17232025-06-0116111610.1038/s41467-025-58897-xIn vivo self-renewal and expansion of quiescent stem cells from a non-human primateJengmin Kang0Abhijnya Kanugovi1M. Pilar J. Stella2Zofija Frimand3Jean Farup4Andoni Urtasun5Shixuan Liu6Anne-Sofie Clausen7Heather Ishak8Summer Bui9Soochi Kim10Camille Ezran11Olga Botvinnik12Ermelinda Porpiglia13Mark A. Krasnow14Antoine de Morree15Thomas A. Rando16Department of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Biomedicine, Aarhus UniversityDepartment of Biomedicine, Aarhus UniversityDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Biochemistry and Howard Hughes Medical Institute, Stanford University School of MedicineDepartment of Biomedicine, Aarhus UniversityDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Biochemistry and Howard Hughes Medical Institute, Stanford University School of MedicineChan Zuckerberg BiohubDepartment of Biomedicine, Aarhus UniversityDepartment of Biochemistry and Howard Hughes Medical Institute, Stanford University School of MedicineDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineDepartment of Neurology and Neurological Sciences, Stanford University School of MedicineAbstract The development of non-human primate models is essential for the fields of developmental and regenerative biology because those models will more closely approximate human biology than do murine models. Based on single cell RNAseq and fluorescence-activated cell sorting, we report the identification and functional characterization of two quiescent stem cell populations (skeletal muscle stem cells (MuSCs) and mesenchymal stem cells termed fibro-adipogenic progenitors (FAPs)) in the non-human primate Microcebus murinus (the gray mouse lemur). We demonstrate in vivo proliferation, differentiation, and self-renewal of both MuSCs and FAPs. By combining cell phenotyping with cross-species molecular profiling and pharmacological interventions, we show that mouse lemur MuSCs and FAPs are more similar to human than to mouse counterparts. We identify unexpected gene targets involved in regulating primate MuSC proliferation and primate FAP adipogenic differentiation. Moreover, we find that the cellular composition of mouse lemur muscle better models human muscle than does macaque (Macaca fascicularis) muscle. Finally, we note that our approach presents as a generalizable pipeline for the identification, isolation, and characterization of stem cell populations in new animal models.https://doi.org/10.1038/s41467-025-58897-x |
| spellingShingle | Jengmin Kang Abhijnya Kanugovi M. Pilar J. Stella Zofija Frimand Jean Farup Andoni Urtasun Shixuan Liu Anne-Sofie Clausen Heather Ishak Summer Bui Soochi Kim Camille Ezran Olga Botvinnik Ermelinda Porpiglia Mark A. Krasnow Antoine de Morree Thomas A. Rando In vivo self-renewal and expansion of quiescent stem cells from a non-human primate Nature Communications |
| title | In vivo self-renewal and expansion of quiescent stem cells from a non-human primate |
| title_full | In vivo self-renewal and expansion of quiescent stem cells from a non-human primate |
| title_fullStr | In vivo self-renewal and expansion of quiescent stem cells from a non-human primate |
| title_full_unstemmed | In vivo self-renewal and expansion of quiescent stem cells from a non-human primate |
| title_short | In vivo self-renewal and expansion of quiescent stem cells from a non-human primate |
| title_sort | in vivo self renewal and expansion of quiescent stem cells from a non human primate |
| url | https://doi.org/10.1038/s41467-025-58897-x |
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