Breadth and function of antibody response to acute SARS-CoV-2 infection in humans.
Serological and plasmablast responses and plasmablast-derived IgG monoclonal antibodies (MAbs) have been analysed in three COVID-19 patients with different clinical severities. Potent humoral responses were detected within 3 weeks of onset of illness in all patients and the serological titre was eli...
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Public Library of Science (PLoS)
2021-02-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1009352&type=printable |
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| author | Kuan-Ying A Huang Tiong Kit Tan Ting-Hua Chen Chung-Guei Huang Ruth Harvey Saira Hussain Cheng-Pin Chen Adam Harding Javier Gilbert-Jaramillo Xu Liu Michael Knight Lisa Schimanski Shin-Ru Shih Yi-Chun Lin Chien-Yu Cheng Shu-Hsing Cheng Yhu-Chering Huang Tzou-Yien Lin Jia-Tsrong Jan Che Ma William James Rodney S Daniels John W McCauley Pramila Rijal Alain R Townsend |
| author_facet | Kuan-Ying A Huang Tiong Kit Tan Ting-Hua Chen Chung-Guei Huang Ruth Harvey Saira Hussain Cheng-Pin Chen Adam Harding Javier Gilbert-Jaramillo Xu Liu Michael Knight Lisa Schimanski Shin-Ru Shih Yi-Chun Lin Chien-Yu Cheng Shu-Hsing Cheng Yhu-Chering Huang Tzou-Yien Lin Jia-Tsrong Jan Che Ma William James Rodney S Daniels John W McCauley Pramila Rijal Alain R Townsend |
| author_sort | Kuan-Ying A Huang |
| collection | DOAJ |
| description | Serological and plasmablast responses and plasmablast-derived IgG monoclonal antibodies (MAbs) have been analysed in three COVID-19 patients with different clinical severities. Potent humoral responses were detected within 3 weeks of onset of illness in all patients and the serological titre was elicited soon after or concomitantly with peripheral plasmablast response. An average of 13.7% and 3.5% of plasmablast-derived MAbs were reactive with virus spike glycoprotein or nucleocapsid, respectively. A subset of anti-spike (10 of 32) antibodies cross-reacted with other betacoronaviruses tested and harboured extensive somatic mutations, indicative of an expansion of memory B cells upon SARS-CoV-2 infection. Fourteen of 32 anti-spike MAbs, including five anti-receptor-binding domain (RBD), three anti-non-RBD S1 and six anti-S2, neutralised wild-type SARS-CoV-2 in independent assays. Anti-RBD MAbs were further grouped into four cross-inhibiting clusters, of which six antibodies from three separate clusters blocked the binding of RBD to ACE2 and five were neutralising. All ACE2-blocking anti-RBD antibodies were isolated from two recovered patients with prolonged fever, which is compatible with substantial ACE2-blocking response in their sera. Finally, the identification of non-competing pairs of neutralising antibodies would offer potential templates for the development of prophylactic and therapeutic agents against SARS-CoV-2. |
| format | Article |
| id | doaj-art-7e9ce5b081804c2fbe263952aa0c1f4f |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2021-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-7e9ce5b081804c2fbe263952aa0c1f4f2025-08-20T02:33:18ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742021-02-01172e100935210.1371/journal.ppat.1009352Breadth and function of antibody response to acute SARS-CoV-2 infection in humans.Kuan-Ying A HuangTiong Kit TanTing-Hua ChenChung-Guei HuangRuth HarveySaira HussainCheng-Pin ChenAdam HardingJavier Gilbert-JaramilloXu LiuMichael KnightLisa SchimanskiShin-Ru ShihYi-Chun LinChien-Yu ChengShu-Hsing ChengYhu-Chering HuangTzou-Yien LinJia-Tsrong JanChe MaWilliam JamesRodney S DanielsJohn W McCauleyPramila RijalAlain R TownsendSerological and plasmablast responses and plasmablast-derived IgG monoclonal antibodies (MAbs) have been analysed in three COVID-19 patients with different clinical severities. Potent humoral responses were detected within 3 weeks of onset of illness in all patients and the serological titre was elicited soon after or concomitantly with peripheral plasmablast response. An average of 13.7% and 3.5% of plasmablast-derived MAbs were reactive with virus spike glycoprotein or nucleocapsid, respectively. A subset of anti-spike (10 of 32) antibodies cross-reacted with other betacoronaviruses tested and harboured extensive somatic mutations, indicative of an expansion of memory B cells upon SARS-CoV-2 infection. Fourteen of 32 anti-spike MAbs, including five anti-receptor-binding domain (RBD), three anti-non-RBD S1 and six anti-S2, neutralised wild-type SARS-CoV-2 in independent assays. Anti-RBD MAbs were further grouped into four cross-inhibiting clusters, of which six antibodies from three separate clusters blocked the binding of RBD to ACE2 and five were neutralising. All ACE2-blocking anti-RBD antibodies were isolated from two recovered patients with prolonged fever, which is compatible with substantial ACE2-blocking response in their sera. Finally, the identification of non-competing pairs of neutralising antibodies would offer potential templates for the development of prophylactic and therapeutic agents against SARS-CoV-2.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1009352&type=printable |
| spellingShingle | Kuan-Ying A Huang Tiong Kit Tan Ting-Hua Chen Chung-Guei Huang Ruth Harvey Saira Hussain Cheng-Pin Chen Adam Harding Javier Gilbert-Jaramillo Xu Liu Michael Knight Lisa Schimanski Shin-Ru Shih Yi-Chun Lin Chien-Yu Cheng Shu-Hsing Cheng Yhu-Chering Huang Tzou-Yien Lin Jia-Tsrong Jan Che Ma William James Rodney S Daniels John W McCauley Pramila Rijal Alain R Townsend Breadth and function of antibody response to acute SARS-CoV-2 infection in humans. PLoS Pathogens |
| title | Breadth and function of antibody response to acute SARS-CoV-2 infection in humans. |
| title_full | Breadth and function of antibody response to acute SARS-CoV-2 infection in humans. |
| title_fullStr | Breadth and function of antibody response to acute SARS-CoV-2 infection in humans. |
| title_full_unstemmed | Breadth and function of antibody response to acute SARS-CoV-2 infection in humans. |
| title_short | Breadth and function of antibody response to acute SARS-CoV-2 infection in humans. |
| title_sort | breadth and function of antibody response to acute sars cov 2 infection in humans |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1009352&type=printable |
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