Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits
The aim of this study was to investigate whether the nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite is suitable to be compounded with VEGF to enhance the axial vascularization in vivo. Thirty rabbits were divided into 2 groups of 15 animals each. In control group, a nHAC/PLA sc...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2014/236259 |
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| author | Xiao Chang Hai Wang Zhihong Wu Xiaojie Lian Fuzhai Cui Xisheng Weng Bo Yang Guixing Qiu Baozhong Zhang |
| author_facet | Xiao Chang Hai Wang Zhihong Wu Xiaojie Lian Fuzhai Cui Xisheng Weng Bo Yang Guixing Qiu Baozhong Zhang |
| author_sort | Xiao Chang |
| collection | DOAJ |
| description | The aim of this study was to investigate whether the nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite is suitable to be compounded with VEGF to enhance the axial vascularization in vivo. Thirty rabbits were divided into 2 groups of 15 animals each. In control group, a nHAC/PLA scaffold slice was vascularized axially by an inserted ligated femoral arteriovenous (AV) bundle in the animal. In experimental group, a slice compounded with VEGF gel was applied. The rabbits were sacrificed at 2 weeks, 6 weeks, and 10 weeks after surgery; the specimens of scaffold slices underwent histomorphometric examination; analysis of the microvessel density (MVD) of both groups was done. The combination with VEGF (Group B) did not enhance the vascularization in early phase (2 and 6 weeks, P>0.05) but worked in later phase (10 weeks, P<0.05). The data of the experiment demonstrated the suitability of the nHAC/PLA composite as carrier for the growth factor VEGF, enabling its sustained release in bioactive form with enough binding efficacy. |
| format | Article |
| id | doaj-art-7e98c8fa4b19479c8cbf6a0a0526c46c |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-7e98c8fa4b19479c8cbf6a0a0526c46c2025-08-20T03:35:36ZengWileyInternational Journal of Polymer Science1687-94221687-94302014-01-01201410.1155/2014/236259236259Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on RabbitsXiao Chang0Hai Wang1Zhihong Wu2Xiaojie Lian3Fuzhai Cui4Xisheng Weng5Bo Yang6Guixing Qiu7Baozhong Zhang8Department of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Materials Science & Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Materials Science & Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, ChinaThe aim of this study was to investigate whether the nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite is suitable to be compounded with VEGF to enhance the axial vascularization in vivo. Thirty rabbits were divided into 2 groups of 15 animals each. In control group, a nHAC/PLA scaffold slice was vascularized axially by an inserted ligated femoral arteriovenous (AV) bundle in the animal. In experimental group, a slice compounded with VEGF gel was applied. The rabbits were sacrificed at 2 weeks, 6 weeks, and 10 weeks after surgery; the specimens of scaffold slices underwent histomorphometric examination; analysis of the microvessel density (MVD) of both groups was done. The combination with VEGF (Group B) did not enhance the vascularization in early phase (2 and 6 weeks, P>0.05) but worked in later phase (10 weeks, P<0.05). The data of the experiment demonstrated the suitability of the nHAC/PLA composite as carrier for the growth factor VEGF, enabling its sustained release in bioactive form with enough binding efficacy.http://dx.doi.org/10.1155/2014/236259 |
| spellingShingle | Xiao Chang Hai Wang Zhihong Wu Xiaojie Lian Fuzhai Cui Xisheng Weng Bo Yang Guixing Qiu Baozhong Zhang Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits International Journal of Polymer Science |
| title | Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits |
| title_full | Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits |
| title_fullStr | Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits |
| title_full_unstemmed | Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits |
| title_short | Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits |
| title_sort | enhancement of vegf on axial vascularization of nano ha collagen pla composites a histomorphometric study on rabbits |
| url | http://dx.doi.org/10.1155/2014/236259 |
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