Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health

ABSTRACT Background Nanoparticles (NPs), such as green cobalt NPs (CoNPs), are easier to pass through cell membranes in animals and interact rapidly with biological systems. Therefore, using green CoNPs is one of the recommendations for enhancing the bioavailability of cobalt, thereby improving its...

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Main Authors: Fayiz M. Reda, Abdullah S. Alawam, Hemat K. Mahmoud, Mohamed T. El‐Saadony, Ayman S. Salah, Hassan A. Rudayni, Ahmed A. Allam, Karima El‐Naggar, Mahmoud Alagawany, Most Khairunnesa
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Veterinary Medicine and Science
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Online Access:https://doi.org/10.1002/vms3.70508
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author Fayiz M. Reda
Abdullah S. Alawam
Hemat K. Mahmoud
Mohamed T. El‐Saadony
Ayman S. Salah
Hassan A. Rudayni
Ahmed A. Allam
Karima El‐Naggar
Mahmoud Alagawany
Most Khairunnesa
author_facet Fayiz M. Reda
Abdullah S. Alawam
Hemat K. Mahmoud
Mohamed T. El‐Saadony
Ayman S. Salah
Hassan A. Rudayni
Ahmed A. Allam
Karima El‐Naggar
Mahmoud Alagawany
Most Khairunnesa
author_sort Fayiz M. Reda
collection DOAJ
description ABSTRACT Background Nanoparticles (NPs), such as green cobalt NPs (CoNPs), are easier to pass through cell membranes in animals and interact rapidly with biological systems. Therefore, using green CoNPs is one of the recommendations for enhancing the bioavailability of cobalt, thereby improving its absorption. Objectives The goal of this study was to explore the influences of biological nano‐cobalt (BNCo) as a feed supplement on broiler growth performance, haematology, blood chemistry, antioxidant activities, immunological status, digestive enzymes and carcass characteristics. Methods A total of 300 Arbour Acre broiler chicks, all unsexed, were distributed into 5 treatment groups, each containing 60 chicks, at random. Five replications of each group were formed, each containing 12 chicks. The first group received a control diet free of BNCo, whereas the second, third, fourth and fifth groups were administered diets fortified with BNCo at concentrations of 100, 200, 300 and 400 ppm, respectively. Results The results illustrated a significant increase in body weight (p < 0.001), body weight gain (p < 0.001) and improvement in feed conversion ratio (p < 0.01) in comparison to the control diet. BNCo supplementation significantly increased the percentage of immune organs, especially the spleen and thymus (p < 0.05, p < 0.01). Moreover, BNCo supplementation significantly increased haemoglobin (Hb) (p < 0.01), red blood cells (RBCs) (p < 0.05) and white blood cells (WBCs) (p < 0.05). In broiler chickens, BNCo supplementation increased serum levels of total protein, albumin and globulin significantly (p < 0.05) and reduced serum levels of aspartate aminotransferase (AST) activity (p < 0.001) and alanine aminotransaminase (ALT) (p < 0.01). The BNCo in feed supplementation raised blood levels of cobalt and markedly improved lipid markers, immunological and antioxidant status (p < 0.05). Furthermore, digestive enzymes were significantly boosted (p < 0.05) by BNCo treatments. Conclusion The results indicated that BNCo supplementation at 200 ppm/kg diet produced the best overall performance, demonstrating its potential as an innovative additive for broiler diets.
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spelling doaj-art-7e8b643d7a044a4ab0ad011cdfbefcd12025-08-20T03:55:59ZengWileyVeterinary Medicine and Science2053-10952025-07-01114n/an/a10.1002/vms3.70508Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and HealthFayiz M. Reda0Abdullah S. Alawam1Hemat K. Mahmoud2Mohamed T. El‐Saadony3Ayman S. Salah4Hassan A. Rudayni5Ahmed A. Allam6Karima El‐Naggar7Mahmoud Alagawany8Most Khairunnesa9Poultry DepartmentFaculty of AgricultureZagazig UniversityZagazig EgyptDepartment of BiologyCollege of ScienceImam Mohammad Ibn Saud Islamic University (IMSIU)RiyadhSaudi ArabiaDepartment of Animal ProductionFaculty of AgricultureZagazig UniversityZagazigEgyptDepartment of Agricultural MicrobiologyFaculty of AgricultureZagazig UniversityZagazigEgyptDepartment of Animal Nutrition and Clinical NutritionFaculty of Veterinary MedicineNew Valley UniversityEl‐KhargaEgyptDepartment of BiologyCollege of ScienceImam Mohammad Ibn Saud Islamic University (IMSIU)RiyadhSaudi ArabiaDepartment of BiologyCollege of ScienceImam Mohammad Ibn Saud Islamic University (IMSIU)RiyadhSaudi ArabiaDepartment of Nutrition and Veterinary Clinical NutritionFaculty of Veterinary MedicineAlexandria UniversityAlexandria GovernorateEgyptPoultry DepartmentFaculty of AgricultureZagazig UniversityZagazig EgyptDepartment of Dairy and Poultry ScienceFaculty of Veterinary Medicine and Animal ScienceGazipur Agricultural UniversityGazipurBangladeshABSTRACT Background Nanoparticles (NPs), such as green cobalt NPs (CoNPs), are easier to pass through cell membranes in animals and interact rapidly with biological systems. Therefore, using green CoNPs is one of the recommendations for enhancing the bioavailability of cobalt, thereby improving its absorption. Objectives The goal of this study was to explore the influences of biological nano‐cobalt (BNCo) as a feed supplement on broiler growth performance, haematology, blood chemistry, antioxidant activities, immunological status, digestive enzymes and carcass characteristics. Methods A total of 300 Arbour Acre broiler chicks, all unsexed, were distributed into 5 treatment groups, each containing 60 chicks, at random. Five replications of each group were formed, each containing 12 chicks. The first group received a control diet free of BNCo, whereas the second, third, fourth and fifth groups were administered diets fortified with BNCo at concentrations of 100, 200, 300 and 400 ppm, respectively. Results The results illustrated a significant increase in body weight (p < 0.001), body weight gain (p < 0.001) and improvement in feed conversion ratio (p < 0.01) in comparison to the control diet. BNCo supplementation significantly increased the percentage of immune organs, especially the spleen and thymus (p < 0.05, p < 0.01). Moreover, BNCo supplementation significantly increased haemoglobin (Hb) (p < 0.01), red blood cells (RBCs) (p < 0.05) and white blood cells (WBCs) (p < 0.05). In broiler chickens, BNCo supplementation increased serum levels of total protein, albumin and globulin significantly (p < 0.05) and reduced serum levels of aspartate aminotransferase (AST) activity (p < 0.001) and alanine aminotransaminase (ALT) (p < 0.01). The BNCo in feed supplementation raised blood levels of cobalt and markedly improved lipid markers, immunological and antioxidant status (p < 0.05). Furthermore, digestive enzymes were significantly boosted (p < 0.05) by BNCo treatments. Conclusion The results indicated that BNCo supplementation at 200 ppm/kg diet produced the best overall performance, demonstrating its potential as an innovative additive for broiler diets.https://doi.org/10.1002/vms3.70508bloodbroilerdigestive enzymesgrowthnano cobalt
spellingShingle Fayiz M. Reda
Abdullah S. Alawam
Hemat K. Mahmoud
Mohamed T. El‐Saadony
Ayman S. Salah
Hassan A. Rudayni
Ahmed A. Allam
Karima El‐Naggar
Mahmoud Alagawany
Most Khairunnesa
Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health
Veterinary Medicine and Science
blood
broiler
digestive enzymes
growth
nano cobalt
title Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health
title_full Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health
title_fullStr Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health
title_full_unstemmed Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health
title_short Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health
title_sort application of green cobalt nanoparticles in the diet of broiler chickens to improve sustainable production and health
topic blood
broiler
digestive enzymes
growth
nano cobalt
url https://doi.org/10.1002/vms3.70508
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