Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures

The importance of thermal acclimation for the Thermal Death Time (TDT) landscape of the common soil living springtail, Folsomia candida (Collembola, Isotomidae), was investigated. To this aim, we acclimated adult springtails at 10 °C (cold-acclimation) and 20 °C (warm-acclimation), respectively. In...

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Main Authors: Micha Wehrli, Stine Slotsbo, Jian Ge, Martin Holmstrup
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Current Research in Insect Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666515824000192
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author Micha Wehrli
Stine Slotsbo
Jian Ge
Martin Holmstrup
author_facet Micha Wehrli
Stine Slotsbo
Jian Ge
Martin Holmstrup
author_sort Micha Wehrli
collection DOAJ
description The importance of thermal acclimation for the Thermal Death Time (TDT) landscape of the common soil living springtail, Folsomia candida (Collembola, Isotomidae), was investigated. To this aim, we acclimated adult springtails at 10 °C (cold-acclimation) and 20 °C (warm-acclimation), respectively. In static thermal tolerance assays, we found the relationship between survival and exposure time at a number of stressful high and low temperatures. Using logistic modelling, we found, at each exposure temperature, the time until 50% mortality had been reached (Lt50). The exponential functions of TDT curves were found by linear regression of log10 Lt50 values against exposure temperature. Results showed that cold acclimation significantly increased cold tolerance and increased the temperature dependence of cold injury accumulation rate (increased the slope by 4 orders of magnitude) in F. candida. Hence, cold acclimation changed the status of this species from chill-susceptible to moderately chill-tolerant. The cellular injury accumulation at sub-zero temperatures was not related to freezing of body water in this study. Congruently, we found a significant negative effect of cold acclimation on heat tolerance and that cold acclimation decreased the thermal sensitivity of the heat injury accumulation rate. Different slopes of the TDT curves between acclimation groups indicated that acclimation shifted the proportional importance of cellular injury mechanisms or the nature of injury mechanisms. Finally, we compare and combine the TDT curves at extreme high and low temperatures with previously published results on longevity at benign temperatures (from 0 to 30 °C) and describe the full thermal niche of F. candida.
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spelling doaj-art-7e8aad935cb749ab9e55028edac62ece2025-08-20T02:39:28ZengElsevierCurrent Research in Insect Science2666-51582024-01-01610008910.1016/j.cris.2024.100089Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperaturesMicha Wehrli0Stine Slotsbo1Jian Ge2Martin Holmstrup3Department of Ecoscience, Aarhus University, C.F. Møllers Allé 4, DK-8000 Aarhus C, DenmarkDepartment of Ecoscience, Aarhus University, C.F. Møllers Allé 4, DK-8000 Aarhus C, DenmarkDepartment of Ecoscience, Aarhus University, C.F. Møllers Allé 4, DK-8000 Aarhus C, DenmarkCorresponding author.; Department of Ecoscience, Aarhus University, C.F. Møllers Allé 4, DK-8000 Aarhus C, DenmarkThe importance of thermal acclimation for the Thermal Death Time (TDT) landscape of the common soil living springtail, Folsomia candida (Collembola, Isotomidae), was investigated. To this aim, we acclimated adult springtails at 10 °C (cold-acclimation) and 20 °C (warm-acclimation), respectively. In static thermal tolerance assays, we found the relationship between survival and exposure time at a number of stressful high and low temperatures. Using logistic modelling, we found, at each exposure temperature, the time until 50% mortality had been reached (Lt50). The exponential functions of TDT curves were found by linear regression of log10 Lt50 values against exposure temperature. Results showed that cold acclimation significantly increased cold tolerance and increased the temperature dependence of cold injury accumulation rate (increased the slope by 4 orders of magnitude) in F. candida. Hence, cold acclimation changed the status of this species from chill-susceptible to moderately chill-tolerant. The cellular injury accumulation at sub-zero temperatures was not related to freezing of body water in this study. Congruently, we found a significant negative effect of cold acclimation on heat tolerance and that cold acclimation decreased the thermal sensitivity of the heat injury accumulation rate. Different slopes of the TDT curves between acclimation groups indicated that acclimation shifted the proportional importance of cellular injury mechanisms or the nature of injury mechanisms. Finally, we compare and combine the TDT curves at extreme high and low temperatures with previously published results on longevity at benign temperatures (from 0 to 30 °C) and describe the full thermal niche of F. candida.http://www.sciencedirect.com/science/article/pii/S2666515824000192CollembolaExtreme temperatureSupercoolingThermal acclimationThermal injuryThermal niche
spellingShingle Micha Wehrli
Stine Slotsbo
Jian Ge
Martin Holmstrup
Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures
Current Research in Insect Science
Collembola
Extreme temperature
Supercooling
Thermal acclimation
Thermal injury
Thermal niche
title Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures
title_full Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures
title_fullStr Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures
title_full_unstemmed Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures
title_short Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures
title_sort acclimation temperature influences the thermal sensitivity of injury accumulation in folsomia candida at extreme low and high temperatures
topic Collembola
Extreme temperature
Supercooling
Thermal acclimation
Thermal injury
Thermal niche
url http://www.sciencedirect.com/science/article/pii/S2666515824000192
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