Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC
<b>Objective:</b> To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. <b>Methods:</b> The HPLC method was performed on an Agilent ZORBAX SB-C<sub>18</sub> column (4.6 mm×250 mm, 5 m) with gradient elution of me...
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The Editorial Office of Food and Machinery
2023-10-01
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| Series: | Shipin yu jixie |
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| author | ZHONG Feifei LI Jing LEI Deqing YANG Yun WU Jianfang OUYANG Li |
| author_facet | ZHONG Feifei LI Jing LEI Deqing YANG Yun WU Jianfang OUYANG Li |
| author_sort | ZHONG Feifei |
| collection | DOAJ |
| description | <b>Objective:</b> To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. <b>Methods:</b> The HPLC method was performed on an Agilent ZORBAX SB-C<sub>18</sub> column (4.6 mm×250 mm, 5 m) with gradient elution of methanol and 0.6% phosphoric acid solution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 255 nm. <b>Results:</b> The contents of rutin, hypericin and quercetin in 20 batches of lotus samples were 1.32~2.06, 1.36~2.43, 1.41~3.26 mg/kg, respectively. The linear range of the concentration of rutin, hypericin and quercetin was 5~200 g/mL (<i>R</i><sup>2</sup>=0.999 9), the three flavonoids showed a good linearity, a high sensitivity, and good serviceability. The relative standard deviation (RSD) for precision, stability, repeatability, and recoveries test were all less than 4.0%, and the average recovery rate of added standard were 96.6%, 96.9%, 97.4%, respecitvely. <b>Conclusion:</b> The method is convenient and accurate, and can be used for the content determination and quality evaluation of flavonoids in Xiang lotus. |
| format | Article |
| id | doaj-art-7e8a346cfdde4b9d81011cf7c3477a19 |
| institution | OA Journals |
| issn | 1003-5788 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | The Editorial Office of Food and Machinery |
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| series | Shipin yu jixie |
| spelling | doaj-art-7e8a346cfdde4b9d81011cf7c3477a192025-08-20T02:23:31ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882023-10-01399576410.13652/j.spjx.1003.5788.2023.80595Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLCZHONG Feifei0LI Jing1LEI Deqing2YANG Yun3WU Jianfang4OUYANG Li5 Changsha Institute for Food and Drug Control, Changsha, Hunan 410008 , China Changsha Institute for Food and Drug Control, Changsha, Hunan 410008 , China Changsha Institute for Food and Drug Control, Changsha, Hunan 410008 , China Changsha Institute for Food and Drug Control, Changsha, Hunan 410008 , China Changsha Institute for Food and Drug Control, Changsha, Hunan 410008 , China Changsha Institute for Food and Drug Control, Changsha, Hunan 410008 , China <b>Objective:</b> To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. <b>Methods:</b> The HPLC method was performed on an Agilent ZORBAX SB-C<sub>18</sub> column (4.6 mm×250 mm, 5 m) with gradient elution of methanol and 0.6% phosphoric acid solution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 255 nm. <b>Results:</b> The contents of rutin, hypericin and quercetin in 20 batches of lotus samples were 1.32~2.06, 1.36~2.43, 1.41~3.26 mg/kg, respectively. The linear range of the concentration of rutin, hypericin and quercetin was 5~200 g/mL (<i>R</i><sup>2</sup>=0.999 9), the three flavonoids showed a good linearity, a high sensitivity, and good serviceability. The relative standard deviation (RSD) for precision, stability, repeatability, and recoveries test were all less than 4.0%, and the average recovery rate of added standard were 96.6%, 96.9%, 97.4%, respecitvely. <b>Conclusion:</b> The method is convenient and accurate, and can be used for the content determination and quality evaluation of flavonoids in Xiang lotus.http://www.ifoodmm.com/spyjxen/article/abstract/20230910 xiang lotus hplc rutin hyperoside quercetin |
| spellingShingle | ZHONG Feifei LI Jing LEI Deqing YANG Yun WU Jianfang OUYANG Li Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC Shipin yu jixie xiang lotus hplc rutin hyperoside quercetin |
| title | Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC |
| title_full | Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC |
| title_fullStr | Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC |
| title_full_unstemmed | Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC |
| title_short | Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC |
| title_sort | determination of rutin hyperoside quercetin in xiang lotus by hplc |
| topic | xiang lotus hplc rutin hyperoside quercetin |
| url | http://www.ifoodmm.com/spyjxen/article/abstract/20230910 |
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