Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC
<b>Objective:</b> To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. <b>Methods:</b> The HPLC method was performed on an Agilent ZORBAX SB-C<sub>18</sub> column (4.6 mm×250 mm, 5 m) with gradient elution of me...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Editorial Office of Food and Machinery
2023-10-01
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| Series: | Shipin yu jixie |
| Subjects: | |
| Online Access: | http://www.ifoodmm.com/spyjxen/article/abstract/20230910 |
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| Summary: | <b>Objective:</b> To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. <b>Methods:</b> The HPLC method was performed on an Agilent ZORBAX SB-C<sub>18</sub> column (4.6 mm×250 mm, 5 m) with gradient elution of methanol and 0.6% phosphoric acid solution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 255 nm. <b>Results:</b> The contents of rutin, hypericin and quercetin in 20 batches of lotus samples were 1.32~2.06, 1.36~2.43, 1.41~3.26 mg/kg, respectively. The linear range of the concentration of rutin, hypericin and quercetin was 5~200 g/mL (<i>R</i><sup>2</sup>=0.999 9), the three flavonoids showed a good linearity, a high sensitivity, and good serviceability. The relative standard deviation (RSD) for precision, stability, repeatability, and recoveries test were all less than 4.0%, and the average recovery rate of added standard were 96.6%, 96.9%, 97.4%, respecitvely. <b>Conclusion:</b> The method is convenient and accurate, and can be used for the content determination and quality evaluation of flavonoids in Xiang lotus. |
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| ISSN: | 1003-5788 |