Platelet-Activating Factor Induces Th17 Cell Differentiation
Th17 cells have been implicated in a number of inflammatory and autoimmune diseases. The phospholipid mediator platelet-activating factor (PAF) is found in increased concentrations in inflammatory lesions and has been shown to induce IL-6 production. We investigated whether PAF could affect the deve...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/913802 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849409161144565760 |
|---|---|
| author | Anne-Marie Drolet Maryse Thivierge Sylvie Turcotte Dominique Hanna Bruno Maynard Jana Stankovà Marek Rola-Pleszczynski |
| author_facet | Anne-Marie Drolet Maryse Thivierge Sylvie Turcotte Dominique Hanna Bruno Maynard Jana Stankovà Marek Rola-Pleszczynski |
| author_sort | Anne-Marie Drolet |
| collection | DOAJ |
| description | Th17 cells have been implicated in a number of inflammatory and autoimmune diseases. The phospholipid mediator platelet-activating factor (PAF) is found in increased concentrations in inflammatory lesions and has been shown to induce IL-6 production. We investigated whether PAF could affect the development of Th17 cells. Picomolar concentrations of PAF induced IL-23, IL-6, and IL-1β expression in monocyte-derived Langerhans cells (LCs) and in keratinocytes. Moreover, when LC were pretreated with PAF and then cocultured with anti-CD3- and anti-CD28-activated T cells, the latter developed a Th17 phenotype, with a significant increase in the expression of the transcriptional regulator RORγt and enhanced expression of IL-17, IL-21, and IL-22. PAF-induced Th17 development was prevented by the PAF receptor antagonist WEB2086 and by neutralizing antibodies to IL-23 and IL-6R. This may constitute a previously unknown stimulus for the development and persistence of inflammatory processes that could be amenable to pharmacologic intervention. |
| format | Article |
| id | doaj-art-7e86544c5dbd4c379238a018dbd7747d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7e86544c5dbd4c379238a018dbd7747d2025-08-20T03:35:36ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/913802913802Platelet-Activating Factor Induces Th17 Cell DifferentiationAnne-Marie Drolet0Maryse Thivierge1Sylvie Turcotte2Dominique Hanna3Bruno Maynard4Jana Stankovà5Marek Rola-Pleszczynski6Immunology Division, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, CanadaImmunology Division, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, CanadaImmunology Division, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, CanadaDermatology Division, Department of Medicine, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, CanadaDermatology Division, Department of Medicine, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, CanadaImmunology Division, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, CanadaImmunology Division, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, CanadaTh17 cells have been implicated in a number of inflammatory and autoimmune diseases. The phospholipid mediator platelet-activating factor (PAF) is found in increased concentrations in inflammatory lesions and has been shown to induce IL-6 production. We investigated whether PAF could affect the development of Th17 cells. Picomolar concentrations of PAF induced IL-23, IL-6, and IL-1β expression in monocyte-derived Langerhans cells (LCs) and in keratinocytes. Moreover, when LC were pretreated with PAF and then cocultured with anti-CD3- and anti-CD28-activated T cells, the latter developed a Th17 phenotype, with a significant increase in the expression of the transcriptional regulator RORγt and enhanced expression of IL-17, IL-21, and IL-22. PAF-induced Th17 development was prevented by the PAF receptor antagonist WEB2086 and by neutralizing antibodies to IL-23 and IL-6R. This may constitute a previously unknown stimulus for the development and persistence of inflammatory processes that could be amenable to pharmacologic intervention.http://dx.doi.org/10.1155/2011/913802 |
| spellingShingle | Anne-Marie Drolet Maryse Thivierge Sylvie Turcotte Dominique Hanna Bruno Maynard Jana Stankovà Marek Rola-Pleszczynski Platelet-Activating Factor Induces Th17 Cell Differentiation Mediators of Inflammation |
| title | Platelet-Activating Factor Induces Th17 Cell Differentiation |
| title_full | Platelet-Activating Factor Induces Th17 Cell Differentiation |
| title_fullStr | Platelet-Activating Factor Induces Th17 Cell Differentiation |
| title_full_unstemmed | Platelet-Activating Factor Induces Th17 Cell Differentiation |
| title_short | Platelet-Activating Factor Induces Th17 Cell Differentiation |
| title_sort | platelet activating factor induces th17 cell differentiation |
| url | http://dx.doi.org/10.1155/2011/913802 |
| work_keys_str_mv | AT annemariedrolet plateletactivatingfactorinducesth17celldifferentiation AT marysethivierge plateletactivatingfactorinducesth17celldifferentiation AT sylvieturcotte plateletactivatingfactorinducesth17celldifferentiation AT dominiquehanna plateletactivatingfactorinducesth17celldifferentiation AT brunomaynard plateletactivatingfactorinducesth17celldifferentiation AT janastankova plateletactivatingfactorinducesth17celldifferentiation AT marekrolapleszczynski plateletactivatingfactorinducesth17celldifferentiation |