Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid Stress
This study elucidates the adaptive mechanisms of <i>Saccharomyces cerevisiae</i> CZ under octanoic acid stress, revealing concentration-dependent growth inhibition (76% lethality at 800 mg/L) and notable tolerance at 600 mg/L. Initial exposure (≤6 h) showed no growth impairment, but prol...
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2025-04-01
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| author | Zhi-Hai Yu Ming-Zhi Shi Wen-Xuan Dong Xiao-Zhu Liu Wei-Yuan Tang Ming-Zheng Huang |
| author_facet | Zhi-Hai Yu Ming-Zhi Shi Wen-Xuan Dong Xiao-Zhu Liu Wei-Yuan Tang Ming-Zheng Huang |
| author_sort | Zhi-Hai Yu |
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| description | This study elucidates the adaptive mechanisms of <i>Saccharomyces cerevisiae</i> CZ under octanoic acid stress, revealing concentration-dependent growth inhibition (76% lethality at 800 mg/L) and notable tolerance at 600 mg/L. Initial exposure (≤6 h) showed no growth impairment, but prolonged treatment induced dose-dependent lethality, accompanied by reduced H<sup>+</sup>/K<sup>+</sup>-ATPase activity and elevated malondialdehyde (MDA) levels, indicative of oxidative damage. Transcriptomic profiling of 5665 genes highlighted the predominant downregulation of ribosomal functions (translation, ribosome biogenesis) and amino acid metabolism pathways (e.g., <i>ARO10</i>, <i>ARO9</i>). Strain-specific regulatory dynamics were observed: (1) <i>TPO1</i>-mediated efflux was active at 400 mg/L but absent at 600 mg/L, suggesting compensatory mechanisms under high stress; (2) <i>HTX1</i>-related genes exhibited bidirectional regulation (downregulated at 400 mg/L vs. upregulated at 600 mg/L), reflecting metabolic flexibility; (3) <i>ACC1</i> downregulation (600 mg/L) and unaltered <i>SFK1</i> expression contrasted with lipid-remodeling strategies in engineered strains; and (4) <i>PMA2</i> suppression diverged from literature-reported <i>PMA1</i> activation, underscoring strain-specific energy reallocation. Suppression of ergosterol biosynthesis and ribosomal genes revealed a trade-off between stress adaptation and biosynthetic processes. These findings reconcile prior contradictions by attributing discrepancies to genetic backgrounds (CZ vs. laboratory/engineered strains) and methodological variations. Unlike strains relying on phospholipid asymmetry or oleic acid overproduction, CZ’s unique tolerance stems from integrated membrane homeostasis (via lipid balance) and metabolic conservation. This work emphasizes the critical role of strain-specific regulatory networks in octanoic acid resistance and provides insights for optimizing yeast robustness through targeted engineering of membrane stability and metabolic adaptability. Future studies should employ multi-omics integration to unravel the dynamic gene regulatory logic underlying these adaptive traits. |
| format | Article |
| id | doaj-art-7e8467bd1f044f83ad60d72745521ca3 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj-art-7e8467bd1f044f83ad60d72745521ca32025-08-20T02:28:28ZengMDPI AGFermentation2311-56372025-04-0111418010.3390/fermentation11040180Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid StressZhi-Hai Yu0Ming-Zhi Shi1Wen-Xuan Dong2Xiao-Zhu Liu3Wei-Yuan Tang4Ming-Zheng Huang5College of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, ChinaCollege of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, ChinaCollege of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, ChinaCollege of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, ChinaCollege of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, ChinaCollege of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, ChinaThis study elucidates the adaptive mechanisms of <i>Saccharomyces cerevisiae</i> CZ under octanoic acid stress, revealing concentration-dependent growth inhibition (76% lethality at 800 mg/L) and notable tolerance at 600 mg/L. Initial exposure (≤6 h) showed no growth impairment, but prolonged treatment induced dose-dependent lethality, accompanied by reduced H<sup>+</sup>/K<sup>+</sup>-ATPase activity and elevated malondialdehyde (MDA) levels, indicative of oxidative damage. Transcriptomic profiling of 5665 genes highlighted the predominant downregulation of ribosomal functions (translation, ribosome biogenesis) and amino acid metabolism pathways (e.g., <i>ARO10</i>, <i>ARO9</i>). Strain-specific regulatory dynamics were observed: (1) <i>TPO1</i>-mediated efflux was active at 400 mg/L but absent at 600 mg/L, suggesting compensatory mechanisms under high stress; (2) <i>HTX1</i>-related genes exhibited bidirectional regulation (downregulated at 400 mg/L vs. upregulated at 600 mg/L), reflecting metabolic flexibility; (3) <i>ACC1</i> downregulation (600 mg/L) and unaltered <i>SFK1</i> expression contrasted with lipid-remodeling strategies in engineered strains; and (4) <i>PMA2</i> suppression diverged from literature-reported <i>PMA1</i> activation, underscoring strain-specific energy reallocation. Suppression of ergosterol biosynthesis and ribosomal genes revealed a trade-off between stress adaptation and biosynthetic processes. These findings reconcile prior contradictions by attributing discrepancies to genetic backgrounds (CZ vs. laboratory/engineered strains) and methodological variations. Unlike strains relying on phospholipid asymmetry or oleic acid overproduction, CZ’s unique tolerance stems from integrated membrane homeostasis (via lipid balance) and metabolic conservation. This work emphasizes the critical role of strain-specific regulatory networks in octanoic acid resistance and provides insights for optimizing yeast robustness through targeted engineering of membrane stability and metabolic adaptability. Future studies should employ multi-omics integration to unravel the dynamic gene regulatory logic underlying these adaptive traits.https://www.mdpi.com/2311-5637/11/4/180<i>Saccharomyces cerevisiae</i>octanoic acid stressgrowth inhibitiontranscriptomic analysisoxidative stress |
| spellingShingle | Zhi-Hai Yu Ming-Zhi Shi Wen-Xuan Dong Xiao-Zhu Liu Wei-Yuan Tang Ming-Zheng Huang Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid Stress Fermentation <i>Saccharomyces cerevisiae</i> octanoic acid stress growth inhibition transcriptomic analysis oxidative stress |
| title | Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid Stress |
| title_full | Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid Stress |
| title_fullStr | Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid Stress |
| title_full_unstemmed | Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid Stress |
| title_short | Transcriptional and Physiological Responses of <i>Saccharomyces cerevisiae</i> CZ to Octanoic Acid Stress |
| title_sort | transcriptional and physiological responses of i saccharomyces cerevisiae i cz to octanoic acid stress |
| topic | <i>Saccharomyces cerevisiae</i> octanoic acid stress growth inhibition transcriptomic analysis oxidative stress |
| url | https://www.mdpi.com/2311-5637/11/4/180 |
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