Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG
<b>Objectives</b>: This study aims to assess the adjuvant effects of lentinan and its combination with Mn(J), a manganese-based colloidal adjuvant, on the BG (fusion protein BfrB-GrpE of <i>Mycobacterium tuberculosis</i>) subunit vaccine. <b>Methods</b>: A rabbit...
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MDPI AG
2025-05-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/13/6/597 |
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| author | Shuai Zhou Yilin Hou Xiaojuan Zhang Zhuoxuan Lv Quanjie Hu Xiaobing Yang Hongxia Niu |
| author_facet | Shuai Zhou Yilin Hou Xiaojuan Zhang Zhuoxuan Lv Quanjie Hu Xiaobing Yang Hongxia Niu |
| author_sort | Shuai Zhou |
| collection | DOAJ |
| description | <b>Objectives</b>: This study aims to assess the adjuvant effects of lentinan and its combination with Mn(J), a manganese-based colloidal adjuvant, on the BG (fusion protein BfrB-GrpE of <i>Mycobacterium tuberculosis</i>) subunit vaccine. <b>Methods</b>: A rabbit skin infection model was established to evaluate the immune protection conferred by the BG–lentinan vaccine, the BG–lentinan/Mn(J) vaccine, and the Bacillus Calmette-Guérin (BCG) vaccine against tuberculosis. Rabbits were vaccinated at weeks 0, 2, and 4. Six weeks post-vaccination, antigen-specific IgG levels were measured, followed by a BCG skin challenge. <b>Results</b>: Both the BG–lentinan and BG–lentinan/Mn(J) vaccines significantly increased antigen-specific IgG levels against BfrB and GrpE in rabbits (<i>p</i> < 0.05). Furthermore, these vaccines accelerated the pathological process following BCG infection. The bacterial load in nodules was notably reduced, with the BG–lentinan vaccine group exhibiting the lowest levels (<i>p</i> < 0.01). <b>Conclusions</b>: Lentinan and its combined adjuvant, lentinan/Mn(J), significantly enhance the immune response elicited by the BG tuberculosis subunit vaccine, providing effective protection. |
| format | Article |
| id | doaj-art-7e82d9a470604d4c8939ec3bb18d403f |
| institution | Kabale University |
| issn | 2076-393X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-7e82d9a470604d4c8939ec3bb18d403f2025-08-20T03:29:48ZengMDPI AGVaccines2076-393X2025-05-0113659710.3390/vaccines13060597Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BGShuai Zhou0Yilin Hou1Xiaojuan Zhang2Zhuoxuan Lv3Quanjie Hu4Xiaobing Yang5Hongxia Niu6Key Laboratory of Blood-Stasis-Toxin Syndrome of Zhejiang Province, School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaKey Laboratory of Blood-Stasis-Toxin Syndrome of Zhejiang Province, School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaKey Laboratory of Blood-Stasis-Toxin Syndrome of Zhejiang Province, School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaKey Laboratory of Blood-Stasis-Toxin Syndrome of Zhejiang Province, School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaKey Laboratory of Blood-Stasis-Toxin Syndrome of Zhejiang Province, School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaDepartment of Rheumatism and Immunology, Huzhou Third Municipal Hospital, The Affiliated Hospital of Huzhou University, Huzhou 313000, ChinaKey Laboratory of Blood-Stasis-Toxin Syndrome of Zhejiang Province, School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China<b>Objectives</b>: This study aims to assess the adjuvant effects of lentinan and its combination with Mn(J), a manganese-based colloidal adjuvant, on the BG (fusion protein BfrB-GrpE of <i>Mycobacterium tuberculosis</i>) subunit vaccine. <b>Methods</b>: A rabbit skin infection model was established to evaluate the immune protection conferred by the BG–lentinan vaccine, the BG–lentinan/Mn(J) vaccine, and the Bacillus Calmette-Guérin (BCG) vaccine against tuberculosis. Rabbits were vaccinated at weeks 0, 2, and 4. Six weeks post-vaccination, antigen-specific IgG levels were measured, followed by a BCG skin challenge. <b>Results</b>: Both the BG–lentinan and BG–lentinan/Mn(J) vaccines significantly increased antigen-specific IgG levels against BfrB and GrpE in rabbits (<i>p</i> < 0.05). Furthermore, these vaccines accelerated the pathological process following BCG infection. The bacterial load in nodules was notably reduced, with the BG–lentinan vaccine group exhibiting the lowest levels (<i>p</i> < 0.01). <b>Conclusions</b>: Lentinan and its combined adjuvant, lentinan/Mn(J), significantly enhance the immune response elicited by the BG tuberculosis subunit vaccine, providing effective protection.https://www.mdpi.com/2076-393X/13/6/597tuberculosissubunit vaccineadjuvanttraditional Chinese medicine adjuvantlentinancombined adjuvant |
| spellingShingle | Shuai Zhou Yilin Hou Xiaojuan Zhang Zhuoxuan Lv Quanjie Hu Xiaobing Yang Hongxia Niu Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG Vaccines tuberculosis subunit vaccine adjuvant traditional Chinese medicine adjuvant lentinan combined adjuvant |
| title | Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG |
| title_full | Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG |
| title_fullStr | Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG |
| title_full_unstemmed | Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG |
| title_short | Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG |
| title_sort | assessment of the adjuvant effects of lentinan on the tuberculosis subunit vaccine bg |
| topic | tuberculosis subunit vaccine adjuvant traditional Chinese medicine adjuvant lentinan combined adjuvant |
| url | https://www.mdpi.com/2076-393X/13/6/597 |
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