Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strain
Abstract Porcine rotavirus (PoRV) is widely prevalent in Chinese pig farms and causes substantial economic losses to the swine industry. Virus isolation remains technically challenging, and the pathogenicity of circulating strains in piglets is incompletely characterized. A total of 481 diarrhoeic s...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | Animal Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s44149-025-00179-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849769325205913600 |
|---|---|
| author | Rui Geng Liguo Gao Shengjin Liu Guangli Hu Zhiqing Zhao Ouyang Peng Hao Zhang Hanqin Shen Yongchang Cao |
| author_facet | Rui Geng Liguo Gao Shengjin Liu Guangli Hu Zhiqing Zhao Ouyang Peng Hao Zhang Hanqin Shen Yongchang Cao |
| author_sort | Rui Geng |
| collection | DOAJ |
| description | Abstract Porcine rotavirus (PoRV) is widely prevalent in Chinese pig farms and causes substantial economic losses to the swine industry. Virus isolation remains technically challenging, and the pathogenicity of circulating strains in piglets is incompletely characterized. A total of 481 diarrhoeic samples were collected from Anhui province, China. PoRV was detected on 83.33% (15/18) of the farms, with a sample-level positivity rate of 28.48% (137/481). Phylogenetic analysis of the VP7 and VP4 genes identified G9 (50.00%) and P[23] (13.33%) as the predominant genotypes. A PoRV strain was successfully isolated and named RVA/Pig/China/GL/2022/G9P[23] (GL/2022), exhibiting a genomic constellation of G9–P[23]–I5–R1–C1–M1–A8–N1–T1–E1–H1. Comparative phylogenetic analyses revealed that GL/2022 shares high homology with both human and porcine RVA lineages, indicating possible interspecies reassortment. Importantly, this study represents the first systematic comparison of the evolutionary dynamics of GL/2022 with those of previously reported G9P[23] strains. Pathogenicity experiments demonstrated that GL/2022 induced severe enteric lesions and 50% mortality in neonatal piglets. These findings highlight PoRV's genetic complexity and zoonotic potential, providing critical insights for disease surveillance and vaccine development. |
| format | Article |
| id | doaj-art-7e8182cc635d49879b98cbb87f23e192 |
| institution | DOAJ |
| issn | 2731-0442 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Diseases |
| spelling | doaj-art-7e8182cc635d49879b98cbb87f23e1922025-08-20T03:03:27ZengBMCAnimal Diseases2731-04422025-07-015111310.1186/s44149-025-00179-6Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strainRui Geng0Liguo Gao1Shengjin Liu2Guangli Hu3Zhiqing Zhao4Ouyang Peng5Hao Zhang6Hanqin Shen7Yongchang Cao8Sate Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityCollege of Animal Science, South China Agricultural UniversityCollege of Animal Science, South China Agricultural UniversitySate Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversitySate Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversitySate Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversitySate Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Enterprise Key Laboratory of Healthy Animal Husbandry and Environment Control, Wen’s Foodstuff Group Co. LtdSate Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityAbstract Porcine rotavirus (PoRV) is widely prevalent in Chinese pig farms and causes substantial economic losses to the swine industry. Virus isolation remains technically challenging, and the pathogenicity of circulating strains in piglets is incompletely characterized. A total of 481 diarrhoeic samples were collected from Anhui province, China. PoRV was detected on 83.33% (15/18) of the farms, with a sample-level positivity rate of 28.48% (137/481). Phylogenetic analysis of the VP7 and VP4 genes identified G9 (50.00%) and P[23] (13.33%) as the predominant genotypes. A PoRV strain was successfully isolated and named RVA/Pig/China/GL/2022/G9P[23] (GL/2022), exhibiting a genomic constellation of G9–P[23]–I5–R1–C1–M1–A8–N1–T1–E1–H1. Comparative phylogenetic analyses revealed that GL/2022 shares high homology with both human and porcine RVA lineages, indicating possible interspecies reassortment. Importantly, this study represents the first systematic comparison of the evolutionary dynamics of GL/2022 with those of previously reported G9P[23] strains. Pathogenicity experiments demonstrated that GL/2022 induced severe enteric lesions and 50% mortality in neonatal piglets. These findings highlight PoRV's genetic complexity and zoonotic potential, providing critical insights for disease surveillance and vaccine development.https://doi.org/10.1186/s44149-025-00179-6Porcine rotavirusG9P[23]Virus isolationPathogenicity |
| spellingShingle | Rui Geng Liguo Gao Shengjin Liu Guangli Hu Zhiqing Zhao Ouyang Peng Hao Zhang Hanqin Shen Yongchang Cao Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strain Animal Diseases Porcine rotavirus G9P[23] Virus isolation Pathogenicity |
| title | Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strain |
| title_full | Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strain |
| title_fullStr | Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strain |
| title_full_unstemmed | Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strain |
| title_short | Surveillance of porcine diarrhea-associated viruses in Anhui province, China (2022–2023) reveals rotavirus predominance and emergence of a highly pathogenic G9P[23] strain |
| title_sort | surveillance of porcine diarrhea associated viruses in anhui province china 2022 2023 reveals rotavirus predominance and emergence of a highly pathogenic g9p 23 strain |
| topic | Porcine rotavirus G9P[23] Virus isolation Pathogenicity |
| url | https://doi.org/10.1186/s44149-025-00179-6 |
| work_keys_str_mv | AT ruigeng surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT liguogao surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT shengjinliu surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT guanglihu surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT zhiqingzhao surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT ouyangpeng surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT haozhang surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT hanqinshen surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain AT yongchangcao surveillanceofporcinediarrheaassociatedvirusesinanhuiprovincechina20222023revealsrotaviruspredominanceandemergenceofahighlypathogenicg9p23strain |