A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain
Bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. The present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated a...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/396304 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849409159456358400 |
|---|---|
| author | Amira M. Embaby Yasmin Heshmat Ahmed Hussein Heba S. Marey |
| author_facet | Amira M. Embaby Yasmin Heshmat Ahmed Hussein Heba S. Marey |
| author_sort | Amira M. Embaby |
| collection | DOAJ |
| description | Bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. The present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated as Bacillus sp. YAS 1 after biochemical and molecular identifications. A sequential statistical approach (Plackett-Burman and Box-Behnken) was employed to optimize bacteriocin (BAC YAS 1) production. Using optimal levels of three key determinants (yeast extract (0.48% (w/v), incubation time (62 hrs), and agitation speed (207 rpm)) in peptone yeast beef based production medium resulted in 1.6-fold enhancement in BAC YAS 1 level (470 AU/mL arbitrary units against Erwinia amylovora). BAC YAS 1 showed activity over a wide range of pH (1–13) and temperature (45–80°C). A wide spectrum antimicrobial activity of BAC YAS 1 against the human pathogens (Clostridium perfringens, Staphylococcus epidermidis, Campylobacter jejuni, Enterobacter aerogenes, Enterococcus sp., Proteus sp., Klebsiella sp., and Salmonella typhimurium), the plant pathogen (E. amylovora), and the food spoiler (Listeria innocua) was demonstrated. On top and above, BAC YAS 1 showed no antimicrobial activity towards lactic acid bacteria (Lactobacillus bulgaricus, L. casei, L. lactis, and L. reuteri). Promising characteristics of BAC YAS 1 prompt its commercialization for efficient utilization in several industries. |
| format | Article |
| id | doaj-art-7e806b7b14d04fd5bf99565b095e4ca1 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7e806b7b14d04fd5bf99565b095e4ca12025-08-20T03:35:36ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/396304396304A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 StrainAmira M. Embaby0Yasmin Heshmat1Ahmed Hussein2Heba S. Marey3Department of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, 163 Horreya Avenue, P.O. Box 832, Chatby 21526, EgyptDepartment of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, 163 Horreya Avenue, P.O. Box 832, Chatby 21526, EgyptDepartment of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, 163 Horreya Avenue, P.O. Box 832, Chatby 21526, EgyptDepartment of Environmental Studies, Institute of Graduate Studies and Research, University of Alexandria, P.O. Box 832, Chatby 21526, EgyptBacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. The present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated as Bacillus sp. YAS 1 after biochemical and molecular identifications. A sequential statistical approach (Plackett-Burman and Box-Behnken) was employed to optimize bacteriocin (BAC YAS 1) production. Using optimal levels of three key determinants (yeast extract (0.48% (w/v), incubation time (62 hrs), and agitation speed (207 rpm)) in peptone yeast beef based production medium resulted in 1.6-fold enhancement in BAC YAS 1 level (470 AU/mL arbitrary units against Erwinia amylovora). BAC YAS 1 showed activity over a wide range of pH (1–13) and temperature (45–80°C). A wide spectrum antimicrobial activity of BAC YAS 1 against the human pathogens (Clostridium perfringens, Staphylococcus epidermidis, Campylobacter jejuni, Enterobacter aerogenes, Enterococcus sp., Proteus sp., Klebsiella sp., and Salmonella typhimurium), the plant pathogen (E. amylovora), and the food spoiler (Listeria innocua) was demonstrated. On top and above, BAC YAS 1 showed no antimicrobial activity towards lactic acid bacteria (Lactobacillus bulgaricus, L. casei, L. lactis, and L. reuteri). Promising characteristics of BAC YAS 1 prompt its commercialization for efficient utilization in several industries.http://dx.doi.org/10.1155/2014/396304 |
| spellingShingle | Amira M. Embaby Yasmin Heshmat Ahmed Hussein Heba S. Marey A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain The Scientific World Journal |
| title | A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain |
| title_full | A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain |
| title_fullStr | A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain |
| title_full_unstemmed | A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain |
| title_short | A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain |
| title_sort | sequential statistical approach towards an optimized production of a broad spectrum bacteriocin substance from a soil bacterium bacillus sp yas 1 strain |
| url | http://dx.doi.org/10.1155/2014/396304 |
| work_keys_str_mv | AT amiramembaby asequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain AT yasminheshmat asequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain AT ahmedhussein asequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain AT hebasmarey asequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain AT amiramembaby sequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain AT yasminheshmat sequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain AT ahmedhussein sequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain AT hebasmarey sequentialstatisticalapproachtowardsanoptimizedproductionofabroadspectrumbacteriocinsubstancefromasoilbacteriumbacillusspyas1strain |