Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study
Abstract Recombinant human growth hormone (rhGH) therapy is the primary treatment for children born small for gestational age (SGA) who fail to show spontaneous catch-up growth by two or four years. While its effects on white adipose tissue are well-documented, this pilot study aimed to investigate...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-89546-4 |
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| author | Lorena González Carolina Gonzalez-Riano Pablo Fernández-García Rubén Cereijo Aina Valls Andrea Soria-Gondek Nativitat Real Belén Requena Joan Bel-Comos Patricia Corrales David Jiménez-Pavón Coral Barbas Francesc Villarroya David Sánchez-Infantes Marta Murillo |
| author_facet | Lorena González Carolina Gonzalez-Riano Pablo Fernández-García Rubén Cereijo Aina Valls Andrea Soria-Gondek Nativitat Real Belén Requena Joan Bel-Comos Patricia Corrales David Jiménez-Pavón Coral Barbas Francesc Villarroya David Sánchez-Infantes Marta Murillo |
| author_sort | Lorena González |
| collection | DOAJ |
| description | Abstract Recombinant human growth hormone (rhGH) therapy is the primary treatment for children born small for gestational age (SGA) who fail to show spontaneous catch-up growth by two or four years. While its effects on white adipose tissue are well-documented, this pilot study aimed to investigate its impact on the lipidome and the thermogenic and endocrine activities of brown adipose tissue (BAT) in SGA children following rhGH treatment. The study involved 11 SGA children divided into two groups: (a) SGA children who were not treated with rhGH (n = 4) and (b) SGA children who received rhGH treatment with Saizen® (n = 7). This second group of seven SGA children was followed for 12 months after initiating rhGH treatment. Interventions included 12-hour fasting blood extraction and infrared thermography at baseline and 3 and 12 months post-treatment. Five appropriate-for-gestational-age (AGA) children served as controls. Exclusion criteria included endocrinological, genetic, or chronic diseases. Untargeted lipidomics analysis was performed using liquid chromatography-mass spectrometry (LC-MS), and serum biomarker levels were measured using ELISA assays. Serum lipidomic analysis revealed that free fatty acids (FFAs) increased to levels close to those of the AGA group after three months of rhGH administration, including polyunsaturated fatty acids, correlating with reduced leptin levels. Elevated levels of 1a,1b-dihomo-PGJ2 and adrenic acid suggested potential aging markers. rhGH treatment also significantly reduced meteorin-like (METRNL) and monocyte chemoattractant protein-1 (MCP1) serum levels to control levels. rhGH influences the serum lipidome, promoting changes in maturation and metabolism. Further research is required to clarify the direct effects of rhGH on specific lipid species and batokines, potentially addressing metabolic disturbances linked to obesity and aging. |
| format | Article |
| id | doaj-art-7e7e1711164e4ec4bc1f6ed0713d7135 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-7e7e1711164e4ec4bc1f6ed0713d71352025-02-09T12:32:30ZengNature PortfolioScientific Reports2045-23222025-02-0115111110.1038/s41598-025-89546-4Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot studyLorena González0Carolina Gonzalez-Riano1Pablo Fernández-García2Rubén Cereijo3Aina Valls4Andrea Soria-Gondek5Nativitat Real6Belén Requena7Joan Bel-Comos8Patricia Corrales9David Jiménez-Pavón10Coral Barbas11Francesc Villarroya12David Sánchez-Infantes13Marta Murillo14Fundació Institut Germans Trias i PujolCentro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización MontepríncipeDepartment of Basic Health Sciences, University Rey Juan Carlos (URJC)Departament of Biochemistry and Molecular Biomedicine, and Institut de Biomedicina (IBUB), University of BarcelonaPediatric Department, Hospital Universitari Germans Trias i PujolPediatric Department, Hospital Universitari Germans Trias i PujolPediatric Department, Hospital Universitari Germans Trias i PujolCentro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización MontepríncipePediatric Department, Hospital Universitari Germans Trias i PujolDepartment of Basic Health Sciences, University Rey Juan Carlos (URJC)MOVE-IT Research Group, Department of Physical Education, Faculty of Education Sciences, University of CadizCentro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización MontepríncipeDepartament of Biochemistry and Molecular Biomedicine, and Institut de Biomedicina (IBUB), University of BarcelonaDepartment of Basic Health Sciences, University Rey Juan Carlos (URJC)Pediatric Department, Hospital Universitari Germans Trias i PujolAbstract Recombinant human growth hormone (rhGH) therapy is the primary treatment for children born small for gestational age (SGA) who fail to show spontaneous catch-up growth by two or four years. While its effects on white adipose tissue are well-documented, this pilot study aimed to investigate its impact on the lipidome and the thermogenic and endocrine activities of brown adipose tissue (BAT) in SGA children following rhGH treatment. The study involved 11 SGA children divided into two groups: (a) SGA children who were not treated with rhGH (n = 4) and (b) SGA children who received rhGH treatment with Saizen® (n = 7). This second group of seven SGA children was followed for 12 months after initiating rhGH treatment. Interventions included 12-hour fasting blood extraction and infrared thermography at baseline and 3 and 12 months post-treatment. Five appropriate-for-gestational-age (AGA) children served as controls. Exclusion criteria included endocrinological, genetic, or chronic diseases. Untargeted lipidomics analysis was performed using liquid chromatography-mass spectrometry (LC-MS), and serum biomarker levels were measured using ELISA assays. Serum lipidomic analysis revealed that free fatty acids (FFAs) increased to levels close to those of the AGA group after three months of rhGH administration, including polyunsaturated fatty acids, correlating with reduced leptin levels. Elevated levels of 1a,1b-dihomo-PGJ2 and adrenic acid suggested potential aging markers. rhGH treatment also significantly reduced meteorin-like (METRNL) and monocyte chemoattractant protein-1 (MCP1) serum levels to control levels. rhGH influences the serum lipidome, promoting changes in maturation and metabolism. Further research is required to clarify the direct effects of rhGH on specific lipid species and batokines, potentially addressing metabolic disturbances linked to obesity and aging.https://doi.org/10.1038/s41598-025-89546-4Recombinant human growth hormoneLipidomeBrown adipose tissueSmall for gestational age |
| spellingShingle | Lorena González Carolina Gonzalez-Riano Pablo Fernández-García Rubén Cereijo Aina Valls Andrea Soria-Gondek Nativitat Real Belén Requena Joan Bel-Comos Patricia Corrales David Jiménez-Pavón Coral Barbas Francesc Villarroya David Sánchez-Infantes Marta Murillo Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study Scientific Reports Recombinant human growth hormone Lipidome Brown adipose tissue Small for gestational age |
| title | Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study |
| title_full | Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study |
| title_fullStr | Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study |
| title_full_unstemmed | Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study |
| title_short | Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study |
| title_sort | effect of rhgh treatment on lipidome and brown fat activity in prepuberal small for gestational age children a pilot study |
| topic | Recombinant human growth hormone Lipidome Brown adipose tissue Small for gestational age |
| url | https://doi.org/10.1038/s41598-025-89546-4 |
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