Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning
Simple sequence repeats (SSRs) were isolated from pearl millet bacterial artificial clones (BACs) without any subcloning steps. SSR sequences were targeted using 3′ end anchored SSR primers. Flanking sequences were isolated by suppression PCR. In this pilot study, 25 SSR markers have been developed...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01312st08 |
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