Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning
Simple sequence repeats (SSRs) were isolated from pearl millet bacterial artificial clones (BACs) without any subcloning steps. SSR sequences were targeted using 3′ end anchored SSR primers. Flanking sequences were isolated by suppression PCR. In this pilot study, 25 SSR markers have been developed...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2001-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01312st08 |
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| _version_ | 1850151887984132096 |
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| author | X. Qi S. Lindup T.S. Pittaway S. Allouis M.D. Gale K.M. Devos |
| author_facet | X. Qi S. Lindup T.S. Pittaway S. Allouis M.D. Gale K.M. Devos |
| author_sort | X. Qi |
| collection | DOAJ |
| description | Simple sequence repeats (SSRs) were isolated from pearl millet bacterial artificial clones (BACs) without any subcloning steps. SSR sequences were targeted using 3′ end anchored SSR primers. Flanking sequences were isolated by suppression PCR. In this pilot study, 25 SSR markers have been developed from 40 BAC pools, comprising a total of 384 clones. This novel way to develop new markers has the added advantage that mapping the SSR markers will anchor individual BACs to the genetic maps and, thus, facilitate the construction of BAC contigs. |
| format | Article |
| id | doaj-art-7e7d0fa86da9400d9fe0927a51cec474 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2001-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-7e7d0fa86da9400d9fe0927a51cec4742025-08-20T02:26:06ZengTaylor & Francis GroupBioTechniques0736-62051940-98182001-08-0131235536210.2144/01312st08Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without SubcloningX. Qi0S. Lindup1T.S. Pittaway2S. Allouis3M.D. Gale4K.M. Devos51John Innes Centre, Norwich, UK1John Innes Centre, Norwich, UK1John Innes Centre, Norwich, UK1John Innes Centre, Norwich, UK1John Innes Centre, Norwich, UK1John Innes Centre, Norwich, UKSimple sequence repeats (SSRs) were isolated from pearl millet bacterial artificial clones (BACs) without any subcloning steps. SSR sequences were targeted using 3′ end anchored SSR primers. Flanking sequences were isolated by suppression PCR. In this pilot study, 25 SSR markers have been developed from 40 BAC pools, comprising a total of 384 clones. This novel way to develop new markers has the added advantage that mapping the SSR markers will anchor individual BACs to the genetic maps and, thus, facilitate the construction of BAC contigs.https://www.future-science.com/doi/10.2144/01312st08 |
| spellingShingle | X. Qi S. Lindup T.S. Pittaway S. Allouis M.D. Gale K.M. Devos Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning BioTechniques |
| title | Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning |
| title_full | Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning |
| title_fullStr | Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning |
| title_full_unstemmed | Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning |
| title_short | Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning |
| title_sort | development of simple sequence repeat markers from bacterial artificial chromosomes without subcloning |
| url | https://www.future-science.com/doi/10.2144/01312st08 |
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