Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function
Phosphotyrosine phosphatases (PTPs) constitute a complex family of enzymes that control the balance of intracellular phosphorylation levels to allow cell responses while avoiding the development of diseases. Despite the relevance of CD4 T cell polarisation and effector function in human autoimmune d...
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| Format: | Article |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/8701042 |
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| author | Patricia Castro-Sánchez Rocio Ramirez-Munoz Pedro Roda-Navarro |
| author_facet | Patricia Castro-Sánchez Rocio Ramirez-Munoz Pedro Roda-Navarro |
| author_sort | Patricia Castro-Sánchez |
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| description | Phosphotyrosine phosphatases (PTPs) constitute a complex family of enzymes that control the balance of intracellular phosphorylation levels to allow cell responses while avoiding the development of diseases. Despite the relevance of CD4 T cell polarisation and effector function in human autoimmune diseases, the expression profile of PTPs during T helper polarisation and restimulation at inflammatory sites has not been assessed. Here, a systematic analysis of the expression profile of PTPs has been carried out during Th1-polarising conditions and upon PKC activation and intracellular raise of Ca2+ in effector cells. Changes in gene expression levels suggest a previously nonnoted regulatory role of several PTPs in Th1 polarisation and effector function. A substantial change in the spatial compartmentalisation of ERK during T cell responses is proposed based on changes in the dose of cytoplasmic and nuclear MAPK phosphatases. Our study also suggests a regulatory role of autoimmune-related PTPs in controlling T helper polarisation in humans. We expect that those PTPs that regulate T helper polarisation will constitute potential targets for intervening CD4 T cell immune responses in order to generate new therapies for the treatment of autoimmune diseases. |
| format | Article |
| id | doaj-art-7e7944ed70594c3aa9f636dc1aefcf8a |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | Journal of Immunology Research |
| spelling | doaj-art-7e7944ed70594c3aa9f636dc1aefcf8a2025-08-20T02:08:35ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/87010428701042Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector FunctionPatricia Castro-Sánchez0Rocio Ramirez-Munoz1Pedro Roda-Navarro2Department of Microbiology I (Immunology), School of Medicine, Complutense University and “12 de Octubre” Health Research Institute, Madrid, SpainDepartment of Microbiology I (Immunology), School of Medicine, Complutense University and “12 de Octubre” Health Research Institute, Madrid, SpainDepartment of Microbiology I (Immunology), School of Medicine, Complutense University and “12 de Octubre” Health Research Institute, Madrid, SpainPhosphotyrosine phosphatases (PTPs) constitute a complex family of enzymes that control the balance of intracellular phosphorylation levels to allow cell responses while avoiding the development of diseases. Despite the relevance of CD4 T cell polarisation and effector function in human autoimmune diseases, the expression profile of PTPs during T helper polarisation and restimulation at inflammatory sites has not been assessed. Here, a systematic analysis of the expression profile of PTPs has been carried out during Th1-polarising conditions and upon PKC activation and intracellular raise of Ca2+ in effector cells. Changes in gene expression levels suggest a previously nonnoted regulatory role of several PTPs in Th1 polarisation and effector function. A substantial change in the spatial compartmentalisation of ERK during T cell responses is proposed based on changes in the dose of cytoplasmic and nuclear MAPK phosphatases. Our study also suggests a regulatory role of autoimmune-related PTPs in controlling T helper polarisation in humans. We expect that those PTPs that regulate T helper polarisation will constitute potential targets for intervening CD4 T cell immune responses in order to generate new therapies for the treatment of autoimmune diseases.http://dx.doi.org/10.1155/2017/8701042 |
| spellingShingle | Patricia Castro-Sánchez Rocio Ramirez-Munoz Pedro Roda-Navarro Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function Journal of Immunology Research |
| title | Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function |
| title_full | Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function |
| title_fullStr | Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function |
| title_full_unstemmed | Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function |
| title_short | Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function |
| title_sort | gene expression profiles of human phosphotyrosine phosphatases consequent to th1 polarisation and effector function |
| url | http://dx.doi.org/10.1155/2017/8701042 |
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