Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application
To improve wound healing, advanced biofabrication techniques are proposed here to develop customized wound patches to release bioactive agents targeting cell function in a controlled manner. Three-dimensional (3D) bioprinted “smart” patches, based on methacrylated gellan gum (GGMA), loaded with tann...
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2025-01-01
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| Online Access: | https://www.mdpi.com/2310-2861/11/1/40 |
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| author | Federica Scalia Alessandra Maria Vitale Domiziana Picone Noemi De Cesare Maria Swiontek Brzezinska Beata Kaczmarek-Szczepanska Alfredo Ronca Barbara Zavan Fabio Bucchieri Marta Anna Szychlinska Ugo D’Amora |
| author_facet | Federica Scalia Alessandra Maria Vitale Domiziana Picone Noemi De Cesare Maria Swiontek Brzezinska Beata Kaczmarek-Szczepanska Alfredo Ronca Barbara Zavan Fabio Bucchieri Marta Anna Szychlinska Ugo D’Amora |
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| description | To improve wound healing, advanced biofabrication techniques are proposed here to develop customized wound patches to release bioactive agents targeting cell function in a controlled manner. Three-dimensional (3D) bioprinted “smart” patches, based on methacrylated gellan gum (GGMA), loaded with tannic acid (TA) or L-ascorbic acid (AA) have been manufactured. To improve stability and degradation time, gellan gum (GG) was chemically modified by grafting methacrylic moieties on the polysaccharide backbone. GGMA patches were characterized through physicochemical, morphological and mechanical evaluation. Kinetics release and antioxidant potential of TA and AA as well as antimicrobial activity against common pathogens <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> in accordance with ISO 22196:2011 are reported. The cytocompatibility of the patches was demonstrated by direct and indirect tests on human dermal fibroblasts (HDF) as per ISO 10993. The positive effect of GGMA patches on cell migration was assessed through a wound healing assay. The results highlighted that the patches are cytocompatible, speed up wound healing and can swell upon contact with the hydration medium and release TA and AA in a controlled way. Overall, the TA- and AA-loaded GGMA patches demonstrated suitable mechanical features; no cytotoxicity; and antioxidant, antimicrobial and wound healing properties, showing satisfactory potential for wound dressing applications. |
| format | Article |
| id | doaj-art-7e7727746680462c915e74c1a4cabc41 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-7e7727746680462c915e74c1a4cabc412025-01-24T13:33:52ZengMDPI AGGels2310-28612025-01-011114010.3390/gels11010040Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing ApplicationFederica Scalia0Alessandra Maria Vitale1Domiziana Picone2Noemi De Cesare3Maria Swiontek Brzezinska4Beata Kaczmarek-Szczepanska5Alfredo Ronca6Barbara Zavan7Fabio Bucchieri8Marta Anna Szychlinska9Ugo D’Amora10Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyInstitute of Polymers, Composites and Biomaterials (IPCB), National Research Council (CNR), 80125 Naples, ItalyDepartment of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, 87-100 Torun, PolandDepartment of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarin 7, 87-100 Torun, PolandInstitute of Polymers, Composites and Biomaterials (IPCB), National Research Council (CNR), 80125 Naples, ItalyDepartment of Translational Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Precision Medicine in Medical, Surgical and Critical Care (MEPRECC), University of Palermo, 90127 Palermo, ItalyInstitute of Polymers, Composites and Biomaterials (IPCB), National Research Council (CNR), 80125 Naples, ItalyTo improve wound healing, advanced biofabrication techniques are proposed here to develop customized wound patches to release bioactive agents targeting cell function in a controlled manner. Three-dimensional (3D) bioprinted “smart” patches, based on methacrylated gellan gum (GGMA), loaded with tannic acid (TA) or L-ascorbic acid (AA) have been manufactured. To improve stability and degradation time, gellan gum (GG) was chemically modified by grafting methacrylic moieties on the polysaccharide backbone. GGMA patches were characterized through physicochemical, morphological and mechanical evaluation. Kinetics release and antioxidant potential of TA and AA as well as antimicrobial activity against common pathogens <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> in accordance with ISO 22196:2011 are reported. The cytocompatibility of the patches was demonstrated by direct and indirect tests on human dermal fibroblasts (HDF) as per ISO 10993. The positive effect of GGMA patches on cell migration was assessed through a wound healing assay. The results highlighted that the patches are cytocompatible, speed up wound healing and can swell upon contact with the hydration medium and release TA and AA in a controlled way. Overall, the TA- and AA-loaded GGMA patches demonstrated suitable mechanical features; no cytotoxicity; and antioxidant, antimicrobial and wound healing properties, showing satisfactory potential for wound dressing applications.https://www.mdpi.com/2310-2861/11/1/40methacrylated gellan gum patchestannic acidL-ascorbic acid3D bioprintingwound healing |
| spellingShingle | Federica Scalia Alessandra Maria Vitale Domiziana Picone Noemi De Cesare Maria Swiontek Brzezinska Beata Kaczmarek-Szczepanska Alfredo Ronca Barbara Zavan Fabio Bucchieri Marta Anna Szychlinska Ugo D’Amora Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application Gels methacrylated gellan gum patches tannic acid L-ascorbic acid 3D bioprinting wound healing |
| title | Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application |
| title_full | Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application |
| title_fullStr | Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application |
| title_full_unstemmed | Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application |
| title_short | Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application |
| title_sort | exploring methacrylated gellan gum 3d bioprinted patches loaded with tannic acid or l ascorbic acid as potential platform for wound dressing application |
| topic | methacrylated gellan gum patches tannic acid L-ascorbic acid 3D bioprinting wound healing |
| url | https://www.mdpi.com/2310-2861/11/1/40 |
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