Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons

Summary: CRISPR-Cas9 technology enables acute gene knockdown and endogenous tagging to study single-synapse function. Here, we present a protocol for depleting alpha-synuclein (α-syn) or visualizing native α-syn with an endogenously inserted fluorescent tag in cultured mouse hippocampal neurons. We...

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Bibliographic Details
Main Authors: Leonardo A. Parra-Rivas, Rohan Sharma, Trinity E. Rust, Hannah O. Bazick, Jared Carlson-Stevermer, Mark J. Zylka, Yuki Ogawa, Subhojit Roy
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Microscopy
Molecular Biology
CRISPR
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672500351X
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