Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes
Abstract Fungi are notorious for causing diseases in plants and domestic animals. ABC transporters play pivotal roles in multidrug resistance in fungi, with some ABC proteins indispensable for the pathogenicity of plant fungal pathogens. However, the roles of ABC proteins in animal pathogenic fungi,...
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BMC
2025-02-01
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| Series: | Cell Communication and Signaling |
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| Online Access: | https://doi.org/10.1186/s12964-024-02022-w |
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| author | Jing Wang Chenwen Xiao Shuang Liang Muhammad Noman Yingying Cai Zhen Zhang Xueming Zhu Rongyao Chai Haiping Qiu Zhongna Hao Yanli Wang Jiaoyu Wang Guolian Bao Guochang Sun Fucheng Lin |
| author_facet | Jing Wang Chenwen Xiao Shuang Liang Muhammad Noman Yingying Cai Zhen Zhang Xueming Zhu Rongyao Chai Haiping Qiu Zhongna Hao Yanli Wang Jiaoyu Wang Guolian Bao Guochang Sun Fucheng Lin |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Abstract Fungi are notorious for causing diseases in plants and domestic animals. ABC transporters play pivotal roles in multidrug resistance in fungi, with some ABC proteins indispensable for the pathogenicity of plant fungal pathogens. However, the roles of ABC proteins in animal pathogenic fungi, and the functional connections between ABC homologues in plant and animal pathogenic fungi are largely obscure. Here, we identified a new ABCG-1 gene, MoCDR1, in rice-blast fungus Magnaporthe oryzae. MoCDR1 disruption caused hypersensitivity to multidrugs, and impaired conidiation, appressorium formation, and pathogenicity. Subsequently, we systematically retrieved ABC proteins in animal pathogenic fungus Trichophyton mentagrophytes and identified TmCdr1, a homologue to MoCdr1. TmCDR1 effectively rescued the drug sensitivity and virulence of ΔMocdr1 and mediated the drug resistance and animal skin infection in T. mentagrophytes. Moreover, MoCDR1 also rescued the defects in drug sensitivity and virulence of ΔTmcdr1. MoCdr1 and TmCdr1 are conserved in structures and functions, and both involved in drug resistance and pathogenicity by analogously regulating gene expression levels related to transporter activity, MAPK signaling pathway, and metabolic processes. Altogether, our results represent the first comprehensive characterization of ABC genes in T. mentagrophytes, establishing a functional correlation between homologous ABC genes in plant and animal pathogenic fungi. |
| format | Article |
| id | doaj-art-7e68db33b00b41ba99ba1e68b55e5eca |
| institution | Kabale University |
| issn | 1478-811X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj-art-7e68db33b00b41ba99ba1e68b55e5eca2025-02-09T12:47:18ZengBMCCell Communication and Signaling1478-811X2025-02-0123112210.1186/s12964-024-02022-wComparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytesJing Wang0Chenwen Xiao1Shuang Liang2Muhammad Noman3Yingying Cai4Zhen Zhang5Xueming Zhu6Rongyao Chai7Haiping Qiu8Zhongna Hao9Yanli Wang10Jiaoyu Wang11Guolian Bao12Guochang Sun13Fucheng Lin14State Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Quality and Safety of Agro-Products Key Laboratory of Agricultural Microbiome of Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesAbstract Fungi are notorious for causing diseases in plants and domestic animals. ABC transporters play pivotal roles in multidrug resistance in fungi, with some ABC proteins indispensable for the pathogenicity of plant fungal pathogens. However, the roles of ABC proteins in animal pathogenic fungi, and the functional connections between ABC homologues in plant and animal pathogenic fungi are largely obscure. Here, we identified a new ABCG-1 gene, MoCDR1, in rice-blast fungus Magnaporthe oryzae. MoCDR1 disruption caused hypersensitivity to multidrugs, and impaired conidiation, appressorium formation, and pathogenicity. Subsequently, we systematically retrieved ABC proteins in animal pathogenic fungus Trichophyton mentagrophytes and identified TmCdr1, a homologue to MoCdr1. TmCDR1 effectively rescued the drug sensitivity and virulence of ΔMocdr1 and mediated the drug resistance and animal skin infection in T. mentagrophytes. Moreover, MoCDR1 also rescued the defects in drug sensitivity and virulence of ΔTmcdr1. MoCdr1 and TmCdr1 are conserved in structures and functions, and both involved in drug resistance and pathogenicity by analogously regulating gene expression levels related to transporter activity, MAPK signaling pathway, and metabolic processes. Altogether, our results represent the first comprehensive characterization of ABC genes in T. mentagrophytes, establishing a functional correlation between homologous ABC genes in plant and animal pathogenic fungi.https://doi.org/10.1186/s12964-024-02022-wMoCDR1TmCDR1ABC transportersMagnaporthe oryzaeTrichophyton mentagrophytes |
| spellingShingle | Jing Wang Chenwen Xiao Shuang Liang Muhammad Noman Yingying Cai Zhen Zhang Xueming Zhu Rongyao Chai Haiping Qiu Zhongna Hao Yanli Wang Jiaoyu Wang Guolian Bao Guochang Sun Fucheng Lin Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes Cell Communication and Signaling MoCDR1 TmCDR1 ABC transporters Magnaporthe oryzae Trichophyton mentagrophytes |
| title | Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes |
| title_full | Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes |
| title_fullStr | Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes |
| title_full_unstemmed | Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes |
| title_short | Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes |
| title_sort | comparative functional analysis of a new cdr1 like abc transporter gene in multidrug resistance and virulence between magnaporthe oryzae and trichophyton mentagrophytes |
| topic | MoCDR1 TmCDR1 ABC transporters Magnaporthe oryzae Trichophyton mentagrophytes |
| url | https://doi.org/10.1186/s12964-024-02022-w |
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