Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms
A selective, accurate, and precise stability-indicating HPLC method for analysis of levamisole hydrochloride in bulk and in their injection and oral solution formulations has been developed and validated in accordance with ICH guidelines. The chromatographic separation was carried out on a C8 column...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/6137897 |
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| author | Amal Abdalraheem Shaza Shantier Imad Abureid Elrasheed A. Gadkariem |
| author_facet | Amal Abdalraheem Shaza Shantier Imad Abureid Elrasheed A. Gadkariem |
| author_sort | Amal Abdalraheem |
| collection | DOAJ |
| description | A selective, accurate, and precise stability-indicating HPLC method for analysis of levamisole hydrochloride in bulk and in their injection and oral solution formulations has been developed and validated in accordance with ICH guidelines. The chromatographic separation was carried out on a C8 column (250 × 4.6 mm with a particle size of 5 micrometer) using a mixture of phosphate buffer pH 8.0 and acetonitrile (70 : 30 v/v) as the mobile phase pumped at a flow rate of 1.5 ml/min with UV detection at 215 nm. The calibration curve was linear over the 10–50 μg/ml concentration range with a correlation coefficient of 1.0000. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.29 μg/ml and 0.89 μg/ml, respectively. The accuracy and the precision of the developed method were significantly good (RSD < 2%). The validity of the proposed method was further confirmed through the statistical comparison of the obtained data with those of the official method. |
| format | Article |
| id | doaj-art-7e674e543b79440b8d6d98a4a79875ec |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-7e674e543b79440b8d6d98a4a79875ec2025-02-03T06:46:31ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/61378976137897Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage FormsAmal Abdalraheem0Shaza Shantier1Imad Abureid2Elrasheed A. Gadkariem3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, P. O. Box 1996, Khartoum, SudanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, P. O. Box 1996, Khartoum, SudanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, P. O. Box 1996, Khartoum, SudanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, P. O. Box 1996, Khartoum, SudanA selective, accurate, and precise stability-indicating HPLC method for analysis of levamisole hydrochloride in bulk and in their injection and oral solution formulations has been developed and validated in accordance with ICH guidelines. The chromatographic separation was carried out on a C8 column (250 × 4.6 mm with a particle size of 5 micrometer) using a mixture of phosphate buffer pH 8.0 and acetonitrile (70 : 30 v/v) as the mobile phase pumped at a flow rate of 1.5 ml/min with UV detection at 215 nm. The calibration curve was linear over the 10–50 μg/ml concentration range with a correlation coefficient of 1.0000. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.29 μg/ml and 0.89 μg/ml, respectively. The accuracy and the precision of the developed method were significantly good (RSD < 2%). The validity of the proposed method was further confirmed through the statistical comparison of the obtained data with those of the official method.http://dx.doi.org/10.1155/2020/6137897 |
| spellingShingle | Amal Abdalraheem Shaza Shantier Imad Abureid Elrasheed A. Gadkariem Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms Journal of Chemistry |
| title | Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms |
| title_full | Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms |
| title_fullStr | Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms |
| title_full_unstemmed | Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms |
| title_short | Stability-Indicating High-Performance Liquid Chromatographic Determination of Levamisole Hydrochloride in Bulk and Dosage Forms |
| title_sort | stability indicating high performance liquid chromatographic determination of levamisole hydrochloride in bulk and dosage forms |
| url | http://dx.doi.org/10.1155/2020/6137897 |
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