Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats
Lazaroid U-74389G is a synthetic 21-aminosteroid with free radical-scavenging and anti-inflammatory effects. This study was designed to evaluate the anti-inflammatory activity of U-74389G on experimental 2,4-dinitrobenzene sulfonic acid hydrate (DNBS)-induced colitis in Wistar rats. Five experimenta...
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Medical University - Pleven
2021-12-01
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| Series: | Journal of Biomedical & Clinical Research |
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| Online Access: | https://jbcr.arphahub.com/article/34598/download/pdf/ |
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| author | Plamen Krastev Alexander Blazhev Galya Tz. Stavreva |
| author_facet | Plamen Krastev Alexander Blazhev Galya Tz. Stavreva |
| author_sort | Plamen Krastev |
| collection | DOAJ |
| description | Lazaroid U-74389G is a synthetic 21-aminosteroid with free radical-scavenging and anti-inflammatory effects. This study was designed to evaluate the anti-inflammatory activity of U-74389G on experimental 2,4-dinitrobenzene sulfonic acid hydrate (DNBS)-induced colitis in Wistar rats. Five experimental groups were formed: a sham control group, a control group, treated with 0.25 ml of 50% ethanol intrarectally (n=8), a group treated with DNBS (30 mg in 0.25 ml of 50% ethanol administered intrarectally, (n=8), a group treated with DNBS and U-74389G at a daily dose of 15 mg/kg i.p. (n=8), and a group treated with DNBS and sulfasalazine, orally, at a dose of 300 mg/kg. During the experiment, the bodyweight of the rats, food intake, stool consistency, and presence of blood in the stool were recorded as markers of clinical condition. On day 6, colonic tissues were excised and scored for macroscopic and histological damage. Blood samples were taken to measure levels of cytokines by ELISA methods. DNBS decreased significantly body weight (from 237.00±2.52 g to 212.50±6.25 g, p=0.04). The rats treated with U-74389G showed greater food intake and weight gain. U-74389G reduced ulceration index: the U-74389G score was 1.25±0.25, and the DNBS score –3.87±0.61; p |
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| institution | Kabale University |
| issn | 1313-9053 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Medical University - Pleven |
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| series | Journal of Biomedical & Clinical Research |
| spelling | doaj-art-7e62c71e5fa943898d28354ace6c508e2025-08-20T03:35:25ZengMedical University - PlevenJournal of Biomedical & Clinical Research1313-90532021-12-0114213113910.2478/jbcr-2021-001834598Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in RatsPlamen KrastevAlexander BlazhevGalya Tz. Stavreva0Medical University PlevenLazaroid U-74389G is a synthetic 21-aminosteroid with free radical-scavenging and anti-inflammatory effects. This study was designed to evaluate the anti-inflammatory activity of U-74389G on experimental 2,4-dinitrobenzene sulfonic acid hydrate (DNBS)-induced colitis in Wistar rats. Five experimental groups were formed: a sham control group, a control group, treated with 0.25 ml of 50% ethanol intrarectally (n=8), a group treated with DNBS (30 mg in 0.25 ml of 50% ethanol administered intrarectally, (n=8), a group treated with DNBS and U-74389G at a daily dose of 15 mg/kg i.p. (n=8), and a group treated with DNBS and sulfasalazine, orally, at a dose of 300 mg/kg. During the experiment, the bodyweight of the rats, food intake, stool consistency, and presence of blood in the stool were recorded as markers of clinical condition. On day 6, colonic tissues were excised and scored for macroscopic and histological damage. Blood samples were taken to measure levels of cytokines by ELISA methods. DNBS decreased significantly body weight (from 237.00±2.52 g to 212.50±6.25 g, p=0.04). The rats treated with U-74389G showed greater food intake and weight gain. U-74389G reduced ulceration index: the U-74389G score was 1.25±0.25, and the DNBS score –3.87±0.61; phttps://jbcr.arphahub.com/article/34598/download/pdf/cytokinesexperimental colitisinflammatory bowe |
| spellingShingle | Plamen Krastev Alexander Blazhev Galya Tz. Stavreva Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats Journal of Biomedical & Clinical Research cytokines experimental colitis inflammatory bowe |
| title | Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats |
| title_full | Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats |
| title_fullStr | Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats |
| title_full_unstemmed | Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats |
| title_short | Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats |
| title_sort | effect of aminosteroid u74389g in a model of inflammatory bowel disease in rats |
| topic | cytokines experimental colitis inflammatory bowe |
| url | https://jbcr.arphahub.com/article/34598/download/pdf/ |
| work_keys_str_mv | AT plamenkrastev effectofaminosteroidu74389ginamodelofinflammatoryboweldiseaseinrats AT alexanderblazhev effectofaminosteroidu74389ginamodelofinflammatoryboweldiseaseinrats AT galyatzstavreva effectofaminosteroidu74389ginamodelofinflammatoryboweldiseaseinrats |