Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae
Introduction: Klebsiella pneumoniae is an encapsulated Gram-negative bacterium that is responsible for numerous infections in healthcare facilities worldwide and is frequently isolated. The World Health Organization has listed K. pneumoniaeas as a critical antibiotic resistant bacterial pathogen fo...
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The Journal of Infection in Developing Countries
2024-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17519 |
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| author | Boukaré Kaboré Ganamé A Ouédraogo Henri S Ouédraogo Hama Cissé Oumarou Zongo Koudbi J Zongo Boukaré Zeba Idrissa Sanou Aly Savadogo |
| author_facet | Boukaré Kaboré Ganamé A Ouédraogo Henri S Ouédraogo Hama Cissé Oumarou Zongo Koudbi J Zongo Boukaré Zeba Idrissa Sanou Aly Savadogo |
| author_sort | Boukaré Kaboré |
| collection | DOAJ |
| description |
Introduction: Klebsiella pneumoniae is an encapsulated Gram-negative bacterium that is responsible for numerous infections in healthcare facilities worldwide and is frequently isolated. The World Health Organization has listed K. pneumoniaeas as a critical antibiotic resistant bacterial pathogen for which new antibiotics are urgently needed. This study aimed to use molecular tools to identify and examine antibiotic resistance in clinical strains of K. pneumoniae.
Methodology: A total of 15 unduplicated K. pneumoniae strains isolated from patient samples with multidrug-resistant (MDR) profiles were subjected to polymerase chain reaction (PCR) to amplify the most common carbapenem resistance genes. (GTG)5 PCR and phylogenetic analysis were performed to identify the genetic relationship between the strains.
Results: All strains yielded a (GTG)5-PCR profile, and this allowed us to group these strains into 8 groups according to the size and number of characteristic bands. Phylogenetic analysis was done using the free software UPGMA and a single bacterial clone with a correlation coefficient of over 97% was identified. New Delhi metallo-beta-lactamase NDM-like (blaNDM) carbapenem resistance genes were detected in three strains of K. pneumoniae, which represented a resistance rate of 20%. However, carbapenemases type A [Klebsiella pneumoniae carbapenemase (KPC) and imipenem-hydrolysing beta-lactamase (IMI), type D [oxacillinase-48 (OXA-48)], and other metallo-β-lactamase [Verona integron-encoded metallo-beta-lactamase (VIM), and enzyme active on imipenem (IMP)] were not detected.
Conclusions: We identified and grouped the blaNDM resistance genes of Klebsiella pneumonia strains.
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| format | Article |
| id | doaj-art-7e600dbf6dc34301b445b0ff471a9f83 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-7e600dbf6dc34301b445b0ff471a9f832025-08-20T02:27:19ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-09-01180910.3855/jidc.17519Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniaeBoukaré Kaboré0Ganamé A Ouédraogo1Henri S Ouédraogo2Hama Cissé3Oumarou Zongo4Koudbi J Zongo5Boukaré Zeba6Idrissa Sanou7Aly Savadogo8Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021Department of Biochemistry and Microbiology, Faculty of Applied Science and Technology, University of Dédougou, Burkina Faso BP 176Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021UFR Health Sciences, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso, 03 BP 7021Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021 Introduction: Klebsiella pneumoniae is an encapsulated Gram-negative bacterium that is responsible for numerous infections in healthcare facilities worldwide and is frequently isolated. The World Health Organization has listed K. pneumoniaeas as a critical antibiotic resistant bacterial pathogen for which new antibiotics are urgently needed. This study aimed to use molecular tools to identify and examine antibiotic resistance in clinical strains of K. pneumoniae. Methodology: A total of 15 unduplicated K. pneumoniae strains isolated from patient samples with multidrug-resistant (MDR) profiles were subjected to polymerase chain reaction (PCR) to amplify the most common carbapenem resistance genes. (GTG)5 PCR and phylogenetic analysis were performed to identify the genetic relationship between the strains. Results: All strains yielded a (GTG)5-PCR profile, and this allowed us to group these strains into 8 groups according to the size and number of characteristic bands. Phylogenetic analysis was done using the free software UPGMA and a single bacterial clone with a correlation coefficient of over 97% was identified. New Delhi metallo-beta-lactamase NDM-like (blaNDM) carbapenem resistance genes were detected in three strains of K. pneumoniae, which represented a resistance rate of 20%. However, carbapenemases type A [Klebsiella pneumoniae carbapenemase (KPC) and imipenem-hydrolysing beta-lactamase (IMI), type D [oxacillinase-48 (OXA-48)], and other metallo-β-lactamase [Verona integron-encoded metallo-beta-lactamase (VIM), and enzyme active on imipenem (IMP)] were not detected. Conclusions: We identified and grouped the blaNDM resistance genes of Klebsiella pneumonia strains. https://jidc.org/index.php/journal/article/view/17519Klebsiella pneumoniaecarbapenemases(GTG)5-PCR |
| spellingShingle | Boukaré Kaboré Ganamé A Ouédraogo Henri S Ouédraogo Hama Cissé Oumarou Zongo Koudbi J Zongo Boukaré Zeba Idrissa Sanou Aly Savadogo Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae Journal of Infection in Developing Countries Klebsiella pneumoniae carbapenemases (GTG)5-PCR |
| title | Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae |
| title_full | Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae |
| title_fullStr | Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae |
| title_full_unstemmed | Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae |
| title_short | Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae |
| title_sort | identification and phylogenetic analysis of carbapenemase genes from clinical strains of klebsiella pneumoniae |
| topic | Klebsiella pneumoniae carbapenemases (GTG)5-PCR |
| url | https://jidc.org/index.php/journal/article/view/17519 |
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