Adverse Outcome Pathways (AOPs) Oriented Approach to Assess In Vitro Hazard of Silica and Lignin Nanomaterials Derived from Biomass Residues

Bio-based nanomaterials (B-NMs), such as silica oxide (SiO<sub>2</sub>)- and lignin (Lig)- based nanoparticles (NPs) derived from biomass waste, have gained attention in the last few years in the view of promoting the sustainability principles in several applications. However, scarce dat...

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Bibliographic Details
Main Authors: Rossella Daniela Bengalli, Maurizio Gualtieri, Mariana Ornelas, Tzanko Tzanov, Paride Mantecca
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Nanomaterials
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Online Access:https://www.mdpi.com/2079-4991/15/7/549
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Summary:Bio-based nanomaterials (B-NMs), such as silica oxide (SiO<sub>2</sub>)- and lignin (Lig)- based nanoparticles (NPs) derived from biomass waste, have gained attention in the last few years in the view of promoting the sustainability principles in several applications. However, scarce data are available about their safety. Thus, a hazard-testing strategy was designed considering as a reference the safe-and-sustainable-by-design (SSbD) framework for chemicals and materials, prioritizing the use of new approach methodologies (NAMs), such as in vitro and adverse outcome pathways (AOPs) approaches, for generating data about the potential hazard of B-NMs. Literature research was performed to identify the adverse outcomes (AOs) related to the selected B-NMs. All the AOPs investigated shared at least oxidative stress, inflammation and cytotoxicity as key events (KEs) that were investigated in lung and immune cells. The tested B-NMs resulted either non-toxic or moderately toxic towards human cells, validating their biocompatibility when compared to reference NMs of similar composition, but not of bio-origin. However, attention should be given to possible AOs deriving after specific functionalization of the B-NMs. Considering the lack of knowledge in this field, the studies performed represent a step forward in the state of the art of the safety assessment of B-NMs.
ISSN:2079-4991