Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans
Abstract Silica nanoparticles (SiO2 NPs) are engineered nanomaterials (ENMs) that have a wide range of application. Increased use in manufacturing has led to concerns about their environmental impact and possible adverse health effects. We conducted a comparative toxicity assessment of bare SiO2-NPs...
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Springer
2020-07-01
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| Series: | Aerosol and Air Quality Research |
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| Online Access: | https://doi.org/10.4209/aaqr.2020.04.0157 |
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| author | Danielle E. Que Wen-Che Hou Micah Belle Marie Yap Ang Chih-Chung Lin |
| author_facet | Danielle E. Que Wen-Che Hou Micah Belle Marie Yap Ang Chih-Chung Lin |
| author_sort | Danielle E. Que |
| collection | DOAJ |
| description | Abstract Silica nanoparticles (SiO2 NPs) are engineered nanomaterials (ENMs) that have a wide range of application. Increased use in manufacturing has led to concerns about their environmental impact and possible adverse health effects. We conducted a comparative toxicity assessment of bare SiO2-NPs and amine-functionalized SiO2 NPs (NH2-SiO2 NPs) utilizing the Caenorhabditis elegans (C. elegans) in vivo model. L1 nematodes were exposed to exposure concentrations of 0.25, 0.5, 2.5, and 5 mg mL−1 until the worms reached the L4 stage. The chronic lethality and lifespan assays revealed a significant decrease in survival rate and lifespan at 2.5 and 5 mg mL−1 for nematodes exposed to bare SiO2 NPs (89% and 88%; 22 days, p < 0.05 and 14 days, p < 0.05) and at 5 mg mL−1 for the NH2-SiO2 NPs-exposed group (86%; 20 days, p < 0.001). Exposure to all SiO2 NP concentrations reduced progeny production to 79–60% while exposure to 2.5 and 5 mg mL−1 of NH2-SiO2 NPs significantly reduced the brood size to 64–63%. Neurobehavioral toxicity was also observed in the SiO2 NP-exposed worms, which displayed significantly decreased head thrashing for up to 92–71% and in the NH2-SiO2 NPs-exposed worms which showed significantly reduced head thrashing movement for up to 91–85% at concentrations of 0.5-5 mg mL−1. Body bending movements were also significantly reduced at 0.5–5 mg mL−1 SiO2 NPs (71–34%) and 2.5–5 mg mL−1 NH2-SiO2 NPs (94–66%). Significant shortening of body size was also observed in nematodes exposed to 0.5–5 mg mL−1 for both SiO2 NPs (93–81%) and NH2-SiO2 NPs (94–88%). Overall, bare SiO2 NPs were observed to be more toxic due to the negatively charged surface OH groups, which may have disrupted protein homeostasis, resulting in the observed toxicities. We suggest that functionality is an important indicator in nanosafety evaluations. |
| format | Article |
| id | doaj-art-7e54088ae7c64affaa2162ec98f768b1 |
| institution | Kabale University |
| issn | 1680-8584 2071-1409 |
| language | English |
| publishDate | 2020-07-01 |
| publisher | Springer |
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| series | Aerosol and Air Quality Research |
| spelling | doaj-art-7e54088ae7c64affaa2162ec98f768b12025-02-09T12:19:37ZengSpringerAerosol and Air Quality Research1680-85842071-14092020-07-012091987200210.4209/aaqr.2020.04.0157Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegansDanielle E. Que0Wen-Che Hou1Micah Belle Marie Yap Ang2Chih-Chung Lin3Department of Environmental Engineering, National Cheng Kung UniversityDepartment of Environmental Engineering, National Cheng Kung UniversityR&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian UniversityDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologyAbstract Silica nanoparticles (SiO2 NPs) are engineered nanomaterials (ENMs) that have a wide range of application. Increased use in manufacturing has led to concerns about their environmental impact and possible adverse health effects. We conducted a comparative toxicity assessment of bare SiO2-NPs and amine-functionalized SiO2 NPs (NH2-SiO2 NPs) utilizing the Caenorhabditis elegans (C. elegans) in vivo model. L1 nematodes were exposed to exposure concentrations of 0.25, 0.5, 2.5, and 5 mg mL−1 until the worms reached the L4 stage. The chronic lethality and lifespan assays revealed a significant decrease in survival rate and lifespan at 2.5 and 5 mg mL−1 for nematodes exposed to bare SiO2 NPs (89% and 88%; 22 days, p < 0.05 and 14 days, p < 0.05) and at 5 mg mL−1 for the NH2-SiO2 NPs-exposed group (86%; 20 days, p < 0.001). Exposure to all SiO2 NP concentrations reduced progeny production to 79–60% while exposure to 2.5 and 5 mg mL−1 of NH2-SiO2 NPs significantly reduced the brood size to 64–63%. Neurobehavioral toxicity was also observed in the SiO2 NP-exposed worms, which displayed significantly decreased head thrashing for up to 92–71% and in the NH2-SiO2 NPs-exposed worms which showed significantly reduced head thrashing movement for up to 91–85% at concentrations of 0.5-5 mg mL−1. Body bending movements were also significantly reduced at 0.5–5 mg mL−1 SiO2 NPs (71–34%) and 2.5–5 mg mL−1 NH2-SiO2 NPs (94–66%). Significant shortening of body size was also observed in nematodes exposed to 0.5–5 mg mL−1 for both SiO2 NPs (93–81%) and NH2-SiO2 NPs (94–88%). Overall, bare SiO2 NPs were observed to be more toxic due to the negatively charged surface OH groups, which may have disrupted protein homeostasis, resulting in the observed toxicities. We suggest that functionality is an important indicator in nanosafety evaluations.https://doi.org/10.4209/aaqr.2020.04.0157Caenorhabditis elegansSilica nanoparticlesNanotoxicityAmine-functionalized silica nanoparticlesHydroxyl-functionalized silica nanoparticles |
| spellingShingle | Danielle E. Que Wen-Che Hou Micah Belle Marie Yap Ang Chih-Chung Lin Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans Aerosol and Air Quality Research Caenorhabditis elegans Silica nanoparticles Nanotoxicity Amine-functionalized silica nanoparticles Hydroxyl-functionalized silica nanoparticles |
| title | Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans |
| title_full | Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans |
| title_fullStr | Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans |
| title_full_unstemmed | Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans |
| title_short | Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans |
| title_sort | toxic effects of hydroxyl and amine functionalized silica nanoparticles sio2 and nh2 sio2 nps on caenorhabditis elegans |
| topic | Caenorhabditis elegans Silica nanoparticles Nanotoxicity Amine-functionalized silica nanoparticles Hydroxyl-functionalized silica nanoparticles |
| url | https://doi.org/10.4209/aaqr.2020.04.0157 |
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