Cloning and characterization of Bombyx mandarina serine protease-like protein
The complementary deoxyribonucleic acid (cDNA) of Bombyx mandarina serine protease-like protein gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 938 bp in length contained an open reading frame (ORF) of 912 bp which encoded 303 amino...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2014-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2013.12.251 |
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| author | Zhang Yongliang Wu Anquan Zhao Jinhui |
| author_facet | Zhang Yongliang Wu Anquan Zhao Jinhui |
| author_sort | Zhang Yongliang |
| collection | DOAJ |
| description | The complementary deoxyribonucleic acid (cDNA) of Bombyx mandarina serine protease-like protein gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 938 bp in length contained an open reading frame (ORF) of 912 bp which encoded 303 amino acid residues. The encoded amino acid sequence had a high identity to the reported sequence of serine protease-like protein from other insects and shared the typical structural features of serine protease family. The serine proteaselike protein gene was only expressed in haemolymph of B. mandarina by RT-PCR and Northern blot analysis. These results provide a molecular basis for further studying the function of this gene in B. mandarina. |
| format | Article |
| id | doaj-art-7e4d8bc51ee341aba9ed5088c0fcfca2 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2014-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-7e4d8bc51ee341aba9ed5088c0fcfca22025-08-20T03:34:21ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552014-03-014011912410.3785/j.issn.1008-9209.2013.12.25110089209Cloning and characterization of Bombyx mandarina serine protease-like proteinZhang YongliangWu AnquanZhao JinhuiThe complementary deoxyribonucleic acid (cDNA) of Bombyx mandarina serine protease-like protein gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 938 bp in length contained an open reading frame (ORF) of 912 bp which encoded 303 amino acid residues. The encoded amino acid sequence had a high identity to the reported sequence of serine protease-like protein from other insects and shared the typical structural features of serine protease family. The serine proteaselike protein gene was only expressed in haemolymph of B. mandarina by RT-PCR and Northern blot analysis. These results provide a molecular basis for further studying the function of this gene in B. mandarina.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2013.12.251Bombyx mandarinaserine protease-like proteintranscriptional expression |
| spellingShingle | Zhang Yongliang Wu Anquan Zhao Jinhui Cloning and characterization of Bombyx mandarina serine protease-like protein 浙江大学学报. 农业与生命科学版 Bombyx mandarina serine protease-like protein transcriptional expression |
| title | Cloning and characterization of Bombyx mandarina serine protease-like protein |
| title_full | Cloning and characterization of Bombyx mandarina serine protease-like protein |
| title_fullStr | Cloning and characterization of Bombyx mandarina serine protease-like protein |
| title_full_unstemmed | Cloning and characterization of Bombyx mandarina serine protease-like protein |
| title_short | Cloning and characterization of Bombyx mandarina serine protease-like protein |
| title_sort | cloning and characterization of bombyx mandarina serine protease like protein |
| topic | Bombyx mandarina serine protease-like protein transcriptional expression |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2013.12.251 |
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