Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applications

Molecular imprinting technology (MIT) is a method for the preparation of artificial receptors for a certain target molecule derived from synthetic polymers. The technique used in synthesizing the molecularly imprinted polymers (MIPs) results in the development of cavities that are properly matched t...

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Main Authors: Alaa Bedair, Mahmoud Hamed, Mahmoud El Hassab, Reham E. Kannouma, Reda M. Abdelhameed, Fotouh R. Mansour
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:Talanta Open
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666831925000335
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author Alaa Bedair
Mahmoud Hamed
Mahmoud El Hassab
Reham E. Kannouma
Reda M. Abdelhameed
Fotouh R. Mansour
author_facet Alaa Bedair
Mahmoud Hamed
Mahmoud El Hassab
Reham E. Kannouma
Reda M. Abdelhameed
Fotouh R. Mansour
author_sort Alaa Bedair
collection DOAJ
description Molecular imprinting technology (MIT) is a method for the preparation of artificial receptors for a certain target molecule derived from synthetic polymers. The technique used in synthesizing the molecularly imprinted polymers (MIPs) results in the development of cavities that are properly matched to the exact structure, sizes, and functional groups of the targeted molecule. As a result, these MIPs are capable of specifically identifying the target molecules. In recent decades, it has been widely employed across various fields such as chromatography, antibody and receptor mimetics, solid-phase extraction, biosensors, and others. However, MIPs faced restrictions in selecting the template, mostly because of the insufficient safety and inadequate stability of the compound, making experimental procedures challenging. Furthermore, when utilizing the target analyte as the template, there is a possibility of template leakage or incomplete removal, leading to potential interference with the experimental outcomes. Consequently, the template molecule for MIT may not always be acceptable for direct utilization. The advent of the MIT dummy template offers a convenient and practical solution to prevent these scenarios. As a result, the dummy MIT (DMIT) has emerged as a promising approach. In this review, the roles of DMIT in sensing and biosensing are discussed.
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institution Kabale University
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publishDate 2025-08-01
publisher Elsevier
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series Talanta Open
spelling doaj-art-7e46fea1be3f4452a705bffd1d9c56192025-08-20T03:42:44ZengElsevierTalanta Open2666-83192025-08-011110043110.1016/j.talo.2025.100431Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applicationsAlaa Bedair0Mahmoud Hamed1Mahmoud El Hassab2Reham E. Kannouma3Reda M. Abdelhameed4Fotouh R. Mansour5Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Monufia 32958, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, Egypt; MIU Chemistry Society (MIU-CS), Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptDepartment of Medicinal Chemistry, Faculty of Pharmacy, King Salman International University (KSIU), South Sinai, EgyptDepartment of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Monufia 32958, EgyptApplied Organic Chemistry Department, Chemical Industries Research institute, National Research Centre, Giza 12622, EgyptDepartment of Medicinal Chemistry, Faculty of Pharmacy, King Salman International University (KSIU), South Sinai, Egypt; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Tanta University, Tanta, 31111, Egypt; Corresponding author at: Elgeish Street, the medical campus of Tanta University, Tanta, Egypt 31111.Molecular imprinting technology (MIT) is a method for the preparation of artificial receptors for a certain target molecule derived from synthetic polymers. The technique used in synthesizing the molecularly imprinted polymers (MIPs) results in the development of cavities that are properly matched to the exact structure, sizes, and functional groups of the targeted molecule. As a result, these MIPs are capable of specifically identifying the target molecules. In recent decades, it has been widely employed across various fields such as chromatography, antibody and receptor mimetics, solid-phase extraction, biosensors, and others. However, MIPs faced restrictions in selecting the template, mostly because of the insufficient safety and inadequate stability of the compound, making experimental procedures challenging. Furthermore, when utilizing the target analyte as the template, there is a possibility of template leakage or incomplete removal, leading to potential interference with the experimental outcomes. Consequently, the template molecule for MIT may not always be acceptable for direct utilization. The advent of the MIT dummy template offers a convenient and practical solution to prevent these scenarios. As a result, the dummy MIT (DMIT) has emerged as a promising approach. In this review, the roles of DMIT in sensing and biosensing are discussed.http://www.sciencedirect.com/science/article/pii/S2666831925000335Dummy templateElectrochemical sensingChemiluminescenceSurface imprintingMolecularly imprinted polymersMIPs
spellingShingle Alaa Bedair
Mahmoud Hamed
Mahmoud El Hassab
Reham E. Kannouma
Reda M. Abdelhameed
Fotouh R. Mansour
Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applications
Talanta Open
Dummy template
Electrochemical sensing
Chemiluminescence
Surface imprinting
Molecularly imprinted polymers
MIPs
title Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applications
title_full Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applications
title_fullStr Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applications
title_full_unstemmed Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applications
title_short Dummy template molecularly imprinted polymer-based sensors in analytical and bioanalytical applications
title_sort dummy template molecularly imprinted polymer based sensors in analytical and bioanalytical applications
topic Dummy template
Electrochemical sensing
Chemiluminescence
Surface imprinting
Molecularly imprinted polymers
MIPs
url http://www.sciencedirect.com/science/article/pii/S2666831925000335
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