An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine Model
With the increasing frequency of ultraviolet (UV) exposure in daily life and the exploration of anti-photoaging strategies, natural plant-derived compounds with anti-skin-aging properties have garnered significant attention. This study aimed to evaluate the efficacy of zein-chitosan-based nanocarrie...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/13/2150 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849428829716611072 |
|---|---|
| author | Fangzhi Xia Fei Wang Liangchun Kuo Pengyu Huang Anyi Liu Gao Wang Xiaomin Tang Kun Guan Ying Xie Junbo Wang |
| author_facet | Fangzhi Xia Fei Wang Liangchun Kuo Pengyu Huang Anyi Liu Gao Wang Xiaomin Tang Kun Guan Ying Xie Junbo Wang |
| author_sort | Fangzhi Xia |
| collection | DOAJ |
| description | With the increasing frequency of ultraviolet (UV) exposure in daily life and the exploration of anti-photoaging strategies, natural plant-derived compounds with anti-skin-aging properties have garnered significant attention. This study aimed to evaluate the efficacy of zein-chitosan-based nanocarriers in enhancing the bioavailability of epigallocatechin gallate (EGCG) and to elucidate its mechanisms in ameliorating skin photoaging. Utilizing a Balb/c mouse model of photoaging, we monitored skin conditions, analyzed skin barrier function parameters, and observed changes in skin tissue structure and collagen fibers through hematoxylin–eosin (H&E) and Masson staining. Immunohistochemical staining was employed to assess COL1A1 levels in the skin, while enzyme-linked immunosorbent assay (ELISA) was used to measure antioxidant enzymes, inflammatory cytokines, matrix metalloproteinases (MMPs), and NF-kB levels. The effects of orally administered EGCG nanoparticles on UV-induced skin aging were investigated. UV exposure significantly increased skin roughness, impaired skin barrier function, thickened the epidermis, reduced collagen content, decreased antioxidant enzyme activity, and elevated levels of inflammatory cytokines, MMPs, and NF-kB in the model group compared to the normal control group. EGCG nanoparticles markedly ameliorated these photoaging manifestations, with some indicators showing superior improvement compared to free EGCG. These findings suggest that EGCG nanoparticles exhibit enhanced anti-photoaging effects over free EGCG, highlighting the potential of nanocarriers as a promising strategy to improve the bioavailability of EGCG. |
| format | Article |
| id | doaj-art-7e3abbf5f6f941ed8a2c8d77070b0fd0 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-7e3abbf5f6f941ed8a2c8d77070b0fd02025-08-20T03:28:33ZengMDPI AGFoods2304-81582025-06-011413215010.3390/foods14132150An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine ModelFangzhi Xia0Fei Wang1Liangchun Kuo2Pengyu Huang3Anyi Liu4Gao Wang5Xiaomin Tang6Kun Guan7Ying Xie8Junbo Wang9Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and Drug Delivery System, and School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and Drug Delivery System, and School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaSchool of Public Health, Peking University, and Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, ChinaWith the increasing frequency of ultraviolet (UV) exposure in daily life and the exploration of anti-photoaging strategies, natural plant-derived compounds with anti-skin-aging properties have garnered significant attention. This study aimed to evaluate the efficacy of zein-chitosan-based nanocarriers in enhancing the bioavailability of epigallocatechin gallate (EGCG) and to elucidate its mechanisms in ameliorating skin photoaging. Utilizing a Balb/c mouse model of photoaging, we monitored skin conditions, analyzed skin barrier function parameters, and observed changes in skin tissue structure and collagen fibers through hematoxylin–eosin (H&E) and Masson staining. Immunohistochemical staining was employed to assess COL1A1 levels in the skin, while enzyme-linked immunosorbent assay (ELISA) was used to measure antioxidant enzymes, inflammatory cytokines, matrix metalloproteinases (MMPs), and NF-kB levels. The effects of orally administered EGCG nanoparticles on UV-induced skin aging were investigated. UV exposure significantly increased skin roughness, impaired skin barrier function, thickened the epidermis, reduced collagen content, decreased antioxidant enzyme activity, and elevated levels of inflammatory cytokines, MMPs, and NF-kB in the model group compared to the normal control group. EGCG nanoparticles markedly ameliorated these photoaging manifestations, with some indicators showing superior improvement compared to free EGCG. These findings suggest that EGCG nanoparticles exhibit enhanced anti-photoaging effects over free EGCG, highlighting the potential of nanocarriers as a promising strategy to improve the bioavailability of EGCG.https://www.mdpi.com/2304-8158/14/13/2150EGCGskin agingultraviolet raysnanoparticle |
| spellingShingle | Fangzhi Xia Fei Wang Liangchun Kuo Pengyu Huang Anyi Liu Gao Wang Xiaomin Tang Kun Guan Ying Xie Junbo Wang An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine Model Foods EGCG skin aging ultraviolet rays nanoparticle |
| title | An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine Model |
| title_full | An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine Model |
| title_fullStr | An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine Model |
| title_full_unstemmed | An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine Model |
| title_short | An Elucidation of the Anti-Photoaging Efficacy and Molecular Mechanisms of Epigallocatechin Gallate Nanoparticles in a Balb/c Murine Model |
| title_sort | elucidation of the anti photoaging efficacy and molecular mechanisms of epigallocatechin gallate nanoparticles in a balb c murine model |
| topic | EGCG skin aging ultraviolet rays nanoparticle |
| url | https://www.mdpi.com/2304-8158/14/13/2150 |
| work_keys_str_mv | AT fangzhixia anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT feiwang anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT liangchunkuo anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT pengyuhuang anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT anyiliu anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT gaowang anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT xiaomintang anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT kunguan anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT yingxie anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT junbowang anelucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT fangzhixia elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT feiwang elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT liangchunkuo elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT pengyuhuang elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT anyiliu elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT gaowang elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT xiaomintang elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT kunguan elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT yingxie elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel AT junbowang elucidationoftheantiphotoagingefficacyandmolecularmechanismsofepigallocatechingallatenanoparticlesinabalbcmurinemodel |