Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils
Anaerobic digestion represents an opportunity for converting organic waste (OW) into valuable products: renewable energy (biogas) and a fertilizer (digestate). However, the long-term effects of digestates on soil biota, especially microorganisms, need to be better documented to understand the impact...
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024171484 |
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| author | Daniela Mora-Salguero Lionel Ranjard Thierry Morvan Samuel Dequiedt Vincent Jean-Baptiste Sophie Sadet-Bourgeteau |
| author_facet | Daniela Mora-Salguero Lionel Ranjard Thierry Morvan Samuel Dequiedt Vincent Jean-Baptiste Sophie Sadet-Bourgeteau |
| author_sort | Daniela Mora-Salguero |
| collection | DOAJ |
| description | Anaerobic digestion represents an opportunity for converting organic waste (OW) into valuable products: renewable energy (biogas) and a fertilizer (digestate). However, the long-term effects of digestates on soil biota, especially microorganisms, need to be better documented to understand the impact of digestate on soil ecosystem functioning and resilience. This study assessed the cumulative effect of repeated pig slurry digestate applications on soil microbial communities over a decade, using an in-situ approach to compare digested feedstock with undigested feedstock and other fertilization treatments. Conducted from 2012 to 2022 at an experimental field site in France, the study involved plots with identical agricultural soil management practices, differing only in fertilization treatments: mineral fertilizer, three different OW (cattle manure, pig slurry, pig slurry digestate), and a control with no organic or mineral fertilizer input. Changes in soil microbial communities were analyzed through molecular microbial biomass and diversity assessments using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes. DNA extraction and molecular analyses were performed on soil samples collected at the start of the trial in 2012 and subsequently in 2017 and 2022. The long-term effects of annual digestate application over a decade include a higher soil microbial diversity in digestate-treated plots than in pig slurry-treated plots, and changes in the soil's microbial community structure and taxonomic composition resembling those observed with mineral fertilizer. Differential abundance analysis at the phylum level revealed few significant differences between digestate- and mineral fertilizer-treated plots for both prokaryotic and fungal communities. Only plots amended with cattle manure exhibited higher soil organic carbon content. Agricultural practices, along with climatic and environmental fluctuations, can significantly influence the response of soil microbial communities, thereby buffering the effects of fertilization treatments. Further research is needed to better understand the effects on soil microbial communities, considering the interactions between repeated digestate applications, different pedological contexts, and agricultural practices. |
| format | Article |
| id | doaj-art-7e3850d202c94b959381b1c2eb399ffa |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-7e3850d202c94b959381b1c2eb399ffa2025-01-17T04:50:12ZengElsevierHeliyon2405-84402025-01-01111e41117Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soilsDaniela Mora-Salguero0Lionel Ranjard1Thierry Morvan2Samuel Dequiedt3Vincent Jean-Baptiste4Sophie Sadet-Bourgeteau5Agroécologie, French National Institute for Agriculture, Food, and Environment (INRAE), Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, FranceAgroécologie, French National Institute for Agriculture, Food, and Environment (INRAE), Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, FranceFrench National Institute for Agriculture, Food, and Environment (INRAE), UMR Sol Agro el hydrosystème Spatialisation, Rennes, FranceAgroécologie, French National Institute for Agriculture, Food, and Environment (INRAE), Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, FranceGaz Réseau de France (GRDF), Paris, FranceAgroécologie, French National Institute for Agriculture, Food, and Environment (INRAE), Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France; Institut Agro Dijon, FranceAnaerobic digestion represents an opportunity for converting organic waste (OW) into valuable products: renewable energy (biogas) and a fertilizer (digestate). However, the long-term effects of digestates on soil biota, especially microorganisms, need to be better documented to understand the impact of digestate on soil ecosystem functioning and resilience. This study assessed the cumulative effect of repeated pig slurry digestate applications on soil microbial communities over a decade, using an in-situ approach to compare digested feedstock with undigested feedstock and other fertilization treatments. Conducted from 2012 to 2022 at an experimental field site in France, the study involved plots with identical agricultural soil management practices, differing only in fertilization treatments: mineral fertilizer, three different OW (cattle manure, pig slurry, pig slurry digestate), and a control with no organic or mineral fertilizer input. Changes in soil microbial communities were analyzed through molecular microbial biomass and diversity assessments using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes. DNA extraction and molecular analyses were performed on soil samples collected at the start of the trial in 2012 and subsequently in 2017 and 2022. The long-term effects of annual digestate application over a decade include a higher soil microbial diversity in digestate-treated plots than in pig slurry-treated plots, and changes in the soil's microbial community structure and taxonomic composition resembling those observed with mineral fertilizer. Differential abundance analysis at the phylum level revealed few significant differences between digestate- and mineral fertilizer-treated plots for both prokaryotic and fungal communities. Only plots amended with cattle manure exhibited higher soil organic carbon content. Agricultural practices, along with climatic and environmental fluctuations, can significantly influence the response of soil microbial communities, thereby buffering the effects of fertilization treatments. Further research is needed to better understand the effects on soil microbial communities, considering the interactions between repeated digestate applications, different pedological contexts, and agricultural practices.http://www.sciencedirect.com/science/article/pii/S2405844024171484Anaerobic digestionSoil microbial communitiesHigh-throughput sequencingLong-term fertilizationAgricultural practices |
| spellingShingle | Daniela Mora-Salguero Lionel Ranjard Thierry Morvan Samuel Dequiedt Vincent Jean-Baptiste Sophie Sadet-Bourgeteau Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils Heliyon Anaerobic digestion Soil microbial communities High-throughput sequencing Long-term fertilization Agricultural practices |
| title | Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils |
| title_full | Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils |
| title_fullStr | Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils |
| title_full_unstemmed | Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils |
| title_short | Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils |
| title_sort | long term effect of repeated application of pig slurry digestate on microbial communities in arable soils |
| topic | Anaerobic digestion Soil microbial communities High-throughput sequencing Long-term fertilization Agricultural practices |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024171484 |
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