Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon
The aim of this study was to compare treatment with cultured bone marrow stromal cells (cBMSCs), bone marrow Mononucleated Cells (BMMNCs), and placebo to repair collagenase-induced tendinitis in horses. In six adult Standardbred horses, 4000 IU of collagenase were injected in the superficial dig...
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Wiley
2010-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2010/250978 |
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| author | Antonio Crovace Luca Lacitignola Giacomo Rossi Edda Francioso |
| author_facet | Antonio Crovace Luca Lacitignola Giacomo Rossi Edda Francioso |
| author_sort | Antonio Crovace |
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| description | The aim of this study was to compare treatment with cultured bone marrow stromal cells (cBMSCs), bone marrow Mononucleated Cells (BMMNCs), and placebo to repair collagenase-induced tendinitis in horses.
In six adult Standardbred horses, 4000 IU of collagenase were injected in the superficial digital flexor tendon (SDFT). Three weeks after collagenase treatment, an average of either 5.5×106 cBMSCs or 1.2×108 BMMNCs, fibrin glue, and saline solution was injected intralesionally in random order.
In cBMSC- and BMMNCS-treated tendons, a high expression of cartilage oligomeric matrix protein (COMP) and type I collagen, but low levels of type III collagen were revealed by immunohistochemistry, with a normal longitudinally oriented fiber pattern. Placebo-treated tendons expressed very low quantities of COMP and type I collagen but large numbers of randomly oriented type III collagen fibers.
Both cBMSC and BMMNCS grafts resulted in a qualitatively similar heling improvement of tendon extracellular matrix, in terms of the type I/III collagen ratio, fiber orientation, and COMP expression. |
| format | Article |
| id | doaj-art-7e361cdac01244d5aa6ec361e55318bf |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-7e361cdac01244d5aa6ec361e55318bf2025-02-03T07:24:21ZengWileyVeterinary Medicine International2042-00482010-01-01201010.4061/2010/250978250978Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor TendonAntonio Crovace0Luca Lacitignola1Giacomo Rossi2Edda Francioso3Dipartimento dell'Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Chirurgia Veterinaria, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, s.p. per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDipartimento dell'Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Chirurgia Veterinaria, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, s.p. per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDipartimento di Scienze Veterinarie, Facoltà di Medicina Veterinaria, Università degli Studi di Camerino, via Circonvallazione 93-95, 62024 Matelica (MC), ItalyDipartimento dell'Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Chirurgia Veterinaria, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, s.p. per Casamassima Km 3, Valenzano, 70010 Bari, ItalyThe aim of this study was to compare treatment with cultured bone marrow stromal cells (cBMSCs), bone marrow Mononucleated Cells (BMMNCs), and placebo to repair collagenase-induced tendinitis in horses. In six adult Standardbred horses, 4000 IU of collagenase were injected in the superficial digital flexor tendon (SDFT). Three weeks after collagenase treatment, an average of either 5.5×106 cBMSCs or 1.2×108 BMMNCs, fibrin glue, and saline solution was injected intralesionally in random order. In cBMSC- and BMMNCS-treated tendons, a high expression of cartilage oligomeric matrix protein (COMP) and type I collagen, but low levels of type III collagen were revealed by immunohistochemistry, with a normal longitudinally oriented fiber pattern. Placebo-treated tendons expressed very low quantities of COMP and type I collagen but large numbers of randomly oriented type III collagen fibers. Both cBMSC and BMMNCS grafts resulted in a qualitatively similar heling improvement of tendon extracellular matrix, in terms of the type I/III collagen ratio, fiber orientation, and COMP expression.http://dx.doi.org/10.4061/2010/250978 |
| spellingShingle | Antonio Crovace Luca Lacitignola Giacomo Rossi Edda Francioso Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon Veterinary Medicine International |
| title | Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon |
| title_full | Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon |
| title_fullStr | Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon |
| title_full_unstemmed | Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon |
| title_short | Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon |
| title_sort | histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in collagenase induced tendinitis of equine superficial digital flexor tendon |
| url | http://dx.doi.org/10.4061/2010/250978 |
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