Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia Patient

We describe the case of a chronic myeloid leukemia (CML) patient with a rare atypical e18a2 BCR::ABL1 transcript. The generation of this transcript was explained by a detailed molecular analysis, including the identification of both chromosomal breakpoints (BCR::ABL1 on der(22) and ABL1::BCR on der(...

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Main Authors: Thomas Pretzsch, Steve Progscha, Thomas Burmeister
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Case Reports in Hematology
Online Access:http://dx.doi.org/10.1155/2024/9439134
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author Thomas Pretzsch
Steve Progscha
Thomas Burmeister
author_facet Thomas Pretzsch
Steve Progscha
Thomas Burmeister
author_sort Thomas Pretzsch
collection DOAJ
description We describe the case of a chronic myeloid leukemia (CML) patient with a rare atypical e18a2 BCR::ABL1 transcript. The generation of this transcript was explained by a detailed molecular analysis, including the identification of both chromosomal breakpoints (BCR::ABL1 on der(22) and ABL1::BCR on der(9)) at the genomic level. The use of a cryptic splice site in intron 1 of ABL1 led to the generation of an in-frame BCR::ABL1 fusion transcript. The diagnostic difficulties caused by this atypical variant and its implications for diagnostic routine are discussed.
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spelling doaj-art-7e33989e82164897a9c8a120460b2edd2025-08-20T02:51:27ZengWileyCase Reports in Hematology2090-65792024-01-01202410.1155/2024/9439134Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia PatientThomas Pretzsch0Steve Progscha1Thomas Burmeister2Labor Berlin Charité-VivantesMVZ Leipzig MitteLabor Berlin Charité-VivantesWe describe the case of a chronic myeloid leukemia (CML) patient with a rare atypical e18a2 BCR::ABL1 transcript. The generation of this transcript was explained by a detailed molecular analysis, including the identification of both chromosomal breakpoints (BCR::ABL1 on der(22) and ABL1::BCR on der(9)) at the genomic level. The use of a cryptic splice site in intron 1 of ABL1 led to the generation of an in-frame BCR::ABL1 fusion transcript. The diagnostic difficulties caused by this atypical variant and its implications for diagnostic routine are discussed.http://dx.doi.org/10.1155/2024/9439134
spellingShingle Thomas Pretzsch
Steve Progscha
Thomas Burmeister
Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia Patient
Case Reports in Hematology
title Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia Patient
title_full Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia Patient
title_fullStr Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia Patient
title_full_unstemmed Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia Patient
title_short Diagnostic Ambiguity Caused by an Atypical e18a2 BCR::ABL1 Transcript in a Chronic Myeloid Leukemia Patient
title_sort diagnostic ambiguity caused by an atypical e18a2 bcr abl1 transcript in a chronic myeloid leukemia patient
url http://dx.doi.org/10.1155/2024/9439134
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AT thomasburmeister diagnosticambiguitycausedbyanatypicale18a2bcrabl1transcriptinachronicmyeloidleukemiapatient