Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs
<b>Background:</b> Early gut microbiome development is critical for neonatal health, and its dysbiosis may impact long-term animal productivity. This study examined the effects of parenteral Ceftiofur Crystalline Free Acid (CCFA) on the composition and diversity of the neonatal lamb feca...
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MDPI AG
2025-04-01
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| author | Mohamed Donia Nasr-Eldin Aref Mohamed Zeineldin Ameer Megahed Benjamin Blair James Lowe Brian Aldridge |
| author_facet | Mohamed Donia Nasr-Eldin Aref Mohamed Zeineldin Ameer Megahed Benjamin Blair James Lowe Brian Aldridge |
| author_sort | Mohamed Donia |
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| description | <b>Background:</b> Early gut microbiome development is critical for neonatal health, and its dysbiosis may impact long-term animal productivity. This study examined the effects of parenteral Ceftiofur Crystalline Free Acid (CCFA) on the composition and diversity of the neonatal lamb fecal microbiome. The emergence of antimicrobial resistance genes associated with CCFA exposure was also investigated. <b>Results:</b> There were distinct microbial populations in the CCFA-treated lambs compared to the control group at each time point, with a highly significant decrease in alpha and beta diversity. The CCFA treatment showed a reduction in several key microbial taxa during nursing, but these differences were diminished by day 56. Unlike the control group, CCFA-treated lambs had core microbes potentially carrying multiple antibiotic resistance genes, including those for beta-lactam, fosfomycin, methicillin, and multidrug resistance. <b>Methods:</b> Twenty-four healthy neonatal lambs were randomly assigned to CCFA-treated (n = 12) and control (n = 12) groups. Fecal samples were collected on days 0, 7, 14, 28, and 56. Genomic DNA was extracted and sequenced using the Illumina MiSeq platform. Microbial composition was analyzed using the MG-RAST pipeline with the RefSeq database. <b>Conclusions:</b> Despite temporary reductions in critical bacterial populations during nursing, the early sheep fecal microbiome demonstrated resilience by repopulating after CCFA antibiotic disruption. While this highlights microbiota stability after short-course antibiotic exposure, the transient disturbance underscores potential risks to early gut health. Importantly, persistent CCFA resistance poses environmental dissemination risks, emphasizing the need for cautious antibiotic use in livestock to mitigate ecological impacts. |
| format | Article |
| id | doaj-art-7e2f1475f1e84ccab87ef54f8baa5832 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-7e2f1475f1e84ccab87ef54f8baa58322025-08-20T03:47:52ZengMDPI AGAntibiotics2079-63822025-04-0114543410.3390/antibiotics14050434Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal LambsMohamed Donia0Nasr-Eldin Aref1Mohamed Zeineldin2Ameer Megahed3Benjamin Blair4James Lowe5Brian Aldridge6Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL 61802, USADepartment of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Assiut University, Assiut 71526, EgyptDepartment of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Benha University, Moshtohor-Toukh 13736, EgyptDepartment of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Benha University, Moshtohor-Toukh 13736, EgyptIntegrated Food Animal Management Systems, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL 61802, USAIntegrated Food Animal Management Systems, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL 61802, USADepartment of Veterinary Medicine and Sciences, College of Veterinary Medicine, Clemson University, Clemson, SC 29634, USA<b>Background:</b> Early gut microbiome development is critical for neonatal health, and its dysbiosis may impact long-term animal productivity. This study examined the effects of parenteral Ceftiofur Crystalline Free Acid (CCFA) on the composition and diversity of the neonatal lamb fecal microbiome. The emergence of antimicrobial resistance genes associated with CCFA exposure was also investigated. <b>Results:</b> There were distinct microbial populations in the CCFA-treated lambs compared to the control group at each time point, with a highly significant decrease in alpha and beta diversity. The CCFA treatment showed a reduction in several key microbial taxa during nursing, but these differences were diminished by day 56. Unlike the control group, CCFA-treated lambs had core microbes potentially carrying multiple antibiotic resistance genes, including those for beta-lactam, fosfomycin, methicillin, and multidrug resistance. <b>Methods:</b> Twenty-four healthy neonatal lambs were randomly assigned to CCFA-treated (n = 12) and control (n = 12) groups. Fecal samples were collected on days 0, 7, 14, 28, and 56. Genomic DNA was extracted and sequenced using the Illumina MiSeq platform. Microbial composition was analyzed using the MG-RAST pipeline with the RefSeq database. <b>Conclusions:</b> Despite temporary reductions in critical bacterial populations during nursing, the early sheep fecal microbiome demonstrated resilience by repopulating after CCFA antibiotic disruption. While this highlights microbiota stability after short-course antibiotic exposure, the transient disturbance underscores potential risks to early gut health. Importantly, persistent CCFA resistance poses environmental dissemination risks, emphasizing the need for cautious antibiotic use in livestock to mitigate ecological impacts.https://www.mdpi.com/2079-6382/14/5/434microbiomelambsceftiofurantibiotic resistance |
| spellingShingle | Mohamed Donia Nasr-Eldin Aref Mohamed Zeineldin Ameer Megahed Benjamin Blair James Lowe Brian Aldridge Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs Antibiotics microbiome lambs ceftiofur antibiotic resistance |
| title | Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs |
| title_full | Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs |
| title_fullStr | Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs |
| title_full_unstemmed | Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs |
| title_short | Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs |
| title_sort | impact of parenteral ceftiofur on developmental dynamics of early life fecal microbiota and antibiotic resistome in neonatal lambs |
| topic | microbiome lambs ceftiofur antibiotic resistance |
| url | https://www.mdpi.com/2079-6382/14/5/434 |
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