Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy
Liver fibrosis represents a progressive pathological condition that can culminate in severe hepatic dysfunction, potentially advancing to cirrhosis and liver cancer. The extent of liver fibrosis is intrinsically associated with the quantity of collagen fibers. Although liver biopsy and ultrasound im...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Photoacoustics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597925000138 |
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| author | Yang Qiu Honghui Li Kun Yu Jiali Chen Li Qi Yinghua Zhao Liming Nie |
| author_facet | Yang Qiu Honghui Li Kun Yu Jiali Chen Li Qi Yinghua Zhao Liming Nie |
| author_sort | Yang Qiu |
| collection | DOAJ |
| description | Liver fibrosis represents a progressive pathological condition that can culminate in severe hepatic dysfunction, potentially advancing to cirrhosis and liver cancer. The extent of liver fibrosis is intrinsically associated with the quantity of collagen fibers. Although liver biopsy and ultrasound imaging are standard diagnostic tools, their application is constrained by risks of significant complications and variability in different investigators, respectively. In this study, we utilized linear dichroism photoacoustic microscopy (LDPAM) to visualize and quantify collagen fibers, which exhibit specific absorption of polarized light, subsequently calculating a collagen fibers degree of dichroism (CDOD) score. We obtained high-resolution images of liver structures, with an emphasis on collagen fibers within the hepatic tissue. Using the CDOD score, we categorized liver fibrosis into three distinct stages: normal, early, and advanced. For validation purposes, collagen fibers were visualized with Sirius-red staining and quantitatively assessed through the collagen proportional area (CPA) score. Our results demonstrated a significant correlation between the CDOD and CPA scores, with a Pearson coefficient of 0.95. This approach presents a promising and non-invasive method for assessing liver fibrosis by quantifying collagen fibers. |
| format | Article |
| id | doaj-art-7e28c76dbea64a14a5865d8498b231c1 |
| institution | Kabale University |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-7e28c76dbea64a14a5865d8498b231c12025-02-10T04:34:22ZengElsevierPhotoacoustics2213-59792025-04-0142100694Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopyYang Qiu0Honghui Li1Kun Yu2Jiali Chen3Li Qi4Yinghua Zhao5Liming Nie6Optical Molecular Imaging Laboratory, Medical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, ChinaOptical Molecular Imaging Laboratory, Medical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, ChinaOptical Molecular Imaging Laboratory, Medical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China; School of Medicine, South China University of Technology, Guangzhou 510006, ChinaOptical Molecular Imaging Laboratory, Medical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China; School of Medicine, South China University of Technology, Guangzhou 510006, ChinaSchool of Biomedical Engineering, Southern Medical University, Guangzhou 510900, ChinaDepartment of Medical Imaging, Third Affiliated Hospital of Southern Medical University (Academy of Orthopedics Guangdong Province), Southern Medical University, Guangzhou 510630, China; Corresponding authors.Optical Molecular Imaging Laboratory, Medical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China; Corresponding authors.Liver fibrosis represents a progressive pathological condition that can culminate in severe hepatic dysfunction, potentially advancing to cirrhosis and liver cancer. The extent of liver fibrosis is intrinsically associated with the quantity of collagen fibers. Although liver biopsy and ultrasound imaging are standard diagnostic tools, their application is constrained by risks of significant complications and variability in different investigators, respectively. In this study, we utilized linear dichroism photoacoustic microscopy (LDPAM) to visualize and quantify collagen fibers, which exhibit specific absorption of polarized light, subsequently calculating a collagen fibers degree of dichroism (CDOD) score. We obtained high-resolution images of liver structures, with an emphasis on collagen fibers within the hepatic tissue. Using the CDOD score, we categorized liver fibrosis into three distinct stages: normal, early, and advanced. For validation purposes, collagen fibers were visualized with Sirius-red staining and quantitatively assessed through the collagen proportional area (CPA) score. Our results demonstrated a significant correlation between the CDOD and CPA scores, with a Pearson coefficient of 0.95. This approach presents a promising and non-invasive method for assessing liver fibrosis by quantifying collagen fibers.http://www.sciencedirect.com/science/article/pii/S2213597925000138Liver fibrosisCollagen fibersLinear dichroismPhotoacoustic imaging |
| spellingShingle | Yang Qiu Honghui Li Kun Yu Jiali Chen Li Qi Yinghua Zhao Liming Nie Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy Photoacoustics Liver fibrosis Collagen fibers Linear dichroism Photoacoustic imaging |
| title | Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy |
| title_full | Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy |
| title_fullStr | Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy |
| title_full_unstemmed | Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy |
| title_short | Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy |
| title_sort | collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy |
| topic | Liver fibrosis Collagen fibers Linear dichroism Photoacoustic imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2213597925000138 |
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