Reliable HPLC Determination of Aflatoxin M1 in Eggs
Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/817091 |
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| author | Mostafa M. H. Khalil Ahmed M. Gomaa Ahmed Salem Sebaei |
| author_facet | Mostafa M. H. Khalil Ahmed M. Gomaa Ahmed Salem Sebaei |
| author_sort | Mostafa M. H. Khalil |
| collection | DOAJ |
| description | Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence detection. The average recovery of aflatoxin M1 at the three different levels 0.05, 0.1, and 0.5 μg/kg varied between 87% and 98%. The method is linear from the limit of quantification 0.05 μg/kg up to 3 μg/kg levels. This method is intended for aflatoxin M1 analyses in eggs simply with minimum toxin lose, excellent recovery, and accurate results with the limit of detection 0.01 μg/kg. |
| format | Article |
| id | doaj-art-7e159a95d3684a71b201a0610905bbd4 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-7e159a95d3684a71b201a0610905bbd42025-08-20T03:35:33ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/817091817091Reliable HPLC Determination of Aflatoxin M1 in EggsMostafa M. H. Khalil0Ahmed M. Gomaa1Ahmed Salem Sebaei2Chemistry Department, Faculty of Science, Ain Shams University, Cairo 11566, EgyptQCAP Laboratory, Agriculture Research Center, Ministry of Agriculture, Giza 12311, EgyptQCAP Laboratory, Agriculture Research Center, Ministry of Agriculture, Giza 12311, EgyptAflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence detection. The average recovery of aflatoxin M1 at the three different levels 0.05, 0.1, and 0.5 μg/kg varied between 87% and 98%. The method is linear from the limit of quantification 0.05 μg/kg up to 3 μg/kg levels. This method is intended for aflatoxin M1 analyses in eggs simply with minimum toxin lose, excellent recovery, and accurate results with the limit of detection 0.01 μg/kg.http://dx.doi.org/10.1155/2013/817091 |
| spellingShingle | Mostafa M. H. Khalil Ahmed M. Gomaa Ahmed Salem Sebaei Reliable HPLC Determination of Aflatoxin M1 in Eggs Journal of Analytical Methods in Chemistry |
| title | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
| title_full | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
| title_fullStr | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
| title_full_unstemmed | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
| title_short | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
| title_sort | reliable hplc determination of aflatoxin m1 in eggs |
| url | http://dx.doi.org/10.1155/2013/817091 |
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