The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid Receptor

Inflammation plays a central role in various pathological conditions, necessitating the search for safer and more effective anti-inflammatory agents. This study investigates the anti-inflammatory activity of caulerpin, a bisindolic alkaloid isolated from <i>Caulerpa racemosa</i>. In vitr...

Full description

Saved in:
Bibliographic Details
Main Authors: Jônatas Sousa Pires dos Santos, Dahara Keyse Carvalho Silva, Vanessa da Silva Oliveira, Sergio Santos Silva Junior, Edivaldo dos Santos Rodrigues, Claudia Valeria Campos de Souza, Sabrina Teixeira Martinez, Osvaldo Andrade Santos-Filho, Cássio Santana Meira, Milena Botelho Pereira Soares
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Marine Drugs
Subjects:
Online Access:https://www.mdpi.com/1660-3397/23/6/232
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849705114496925696
author Jônatas Sousa Pires dos Santos
Dahara Keyse Carvalho Silva
Vanessa da Silva Oliveira
Sergio Santos Silva Junior
Edivaldo dos Santos Rodrigues
Claudia Valeria Campos de Souza
Sabrina Teixeira Martinez
Osvaldo Andrade Santos-Filho
Cássio Santana Meira
Milena Botelho Pereira Soares
author_facet Jônatas Sousa Pires dos Santos
Dahara Keyse Carvalho Silva
Vanessa da Silva Oliveira
Sergio Santos Silva Junior
Edivaldo dos Santos Rodrigues
Claudia Valeria Campos de Souza
Sabrina Teixeira Martinez
Osvaldo Andrade Santos-Filho
Cássio Santana Meira
Milena Botelho Pereira Soares
author_sort Jônatas Sousa Pires dos Santos
collection DOAJ
description Inflammation plays a central role in various pathological conditions, necessitating the search for safer and more effective anti-inflammatory agents. This study investigates the anti-inflammatory activity of caulerpin, a bisindolic alkaloid isolated from <i>Caulerpa racemosa</i>. In vitro assays demonstrated that caulerpin significantly reduced nitric oxide, TNF-α, IL-6, and IL-12 levels in macrophages stimulated with LPS + IFN-γ, without affecting cell viability. In silico toxicity predictions using Protox 3.0 reinforce a favorable safety profile of caulerpin. Molecular docking and molecular dynamics simulations revealed its high-affinity binding to the glucocorticoid receptor ligand-binding domain (GR-LBD), suggesting a mechanism of action similar to dexamethasone. The involvement of the glucocorticoid receptor was confirmed by the partial reversal of caulerpin’s effects upon RU486 treatment. In vivo, caulerpin exhibited a favorable safety profile, with no signs of acute toxicity at an oral dose of 100 mg/kg. Moreover, in a mouse model of endotoxic shock, caulerpin administration significantly improved survival rates in a dose-dependent manner, providing complete protection at 4 mg/kg. These findings highlight caulerpin as a promising candidate for the development of novel anti-inflammatory therapies. Further studies are warranted to explore its pharmacokinetics, optimize its structure, and evaluate its efficacy in chronic inflammatory diseases.
format Article
id doaj-art-7e139deafbbd4bd88c8b3fab7ea31100
institution DOAJ
issn 1660-3397
language English
publishDate 2025-05-01
publisher MDPI AG
record_format Article
series Marine Drugs
spelling doaj-art-7e139deafbbd4bd88c8b3fab7ea311002025-08-20T03:16:33ZengMDPI AGMarine Drugs1660-33972025-05-0123623210.3390/md23060232The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid ReceptorJônatas Sousa Pires dos Santos0Dahara Keyse Carvalho Silva1Vanessa da Silva Oliveira2Sergio Santos Silva Junior3Edivaldo dos Santos Rodrigues4Claudia Valeria Campos de Souza5Sabrina Teixeira Martinez6Osvaldo Andrade Santos-Filho7Cássio Santana Meira8Milena Botelho Pereira Soares9Department of Life Sciences, State University of Bahia (UNEB), Salvador 40020-000, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation, FIOCRUZ, Salvador 40296-710, BrazilDepartment of Life Sciences, State University of Bahia (UNEB), Salvador 40020-000, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation, FIOCRUZ, Salvador 40296-710, BrazilLaboratory of Molecular Modeling and Computational Structural Biology, Walter Mors Natural Products Research Institute, Health Sciences Center, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-599, BrazilLaboratory of Molecular Modeling and Computational Structural Biology, Walter Mors Natural Products Research Institute, Health Sciences Center, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-599, BrazilInstitute of Innovation in Advanced Health Systems (ISI SAS), University SENAI/CIMATEC, Salvador 41650-010, BrazilLaboratory of Molecular Modeling and Computational Structural Biology, Walter Mors Natural Products Research Institute, Health Sciences Center, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-599, BrazilDepartment of Life Sciences, State University of Bahia (UNEB), Salvador 40020-000, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation, FIOCRUZ, Salvador 40296-710, BrazilInflammation plays a central role in various pathological conditions, necessitating the search for safer and more effective anti-inflammatory agents. This study investigates the anti-inflammatory activity of caulerpin, a bisindolic alkaloid isolated from <i>Caulerpa racemosa</i>. In vitro assays demonstrated that caulerpin significantly reduced nitric oxide, TNF-α, IL-6, and IL-12 levels in macrophages stimulated with LPS + IFN-γ, without affecting cell viability. In silico toxicity predictions using Protox 3.0 reinforce a favorable safety profile of caulerpin. Molecular docking and molecular dynamics simulations revealed its high-affinity binding to the glucocorticoid receptor ligand-binding domain (GR-LBD), suggesting a mechanism of action similar to dexamethasone. The involvement of the glucocorticoid receptor was confirmed by the partial reversal of caulerpin’s effects upon RU486 treatment. In vivo, caulerpin exhibited a favorable safety profile, with no signs of acute toxicity at an oral dose of 100 mg/kg. Moreover, in a mouse model of endotoxic shock, caulerpin administration significantly improved survival rates in a dose-dependent manner, providing complete protection at 4 mg/kg. These findings highlight caulerpin as a promising candidate for the development of novel anti-inflammatory therapies. Further studies are warranted to explore its pharmacokinetics, optimize its structure, and evaluate its efficacy in chronic inflammatory diseases.https://www.mdpi.com/1660-3397/23/6/232caulerpinglucocorticoid receptormacrophagesanti-inflammatory activity
spellingShingle Jônatas Sousa Pires dos Santos
Dahara Keyse Carvalho Silva
Vanessa da Silva Oliveira
Sergio Santos Silva Junior
Edivaldo dos Santos Rodrigues
Claudia Valeria Campos de Souza
Sabrina Teixeira Martinez
Osvaldo Andrade Santos-Filho
Cássio Santana Meira
Milena Botelho Pereira Soares
The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid Receptor
Marine Drugs
caulerpin
glucocorticoid receptor
macrophages
anti-inflammatory activity
title The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid Receptor
title_full The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid Receptor
title_fullStr The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid Receptor
title_full_unstemmed The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid Receptor
title_short The Alkaloid Caulerpin Exhibits Potent and Selective Anti-Inflammatory Activity Through Interaction with the Glucocorticoid Receptor
title_sort alkaloid caulerpin exhibits potent and selective anti inflammatory activity through interaction with the glucocorticoid receptor
topic caulerpin
glucocorticoid receptor
macrophages
anti-inflammatory activity
url https://www.mdpi.com/1660-3397/23/6/232
work_keys_str_mv AT jonatassousapiresdossantos thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT daharakeysecarvalhosilva thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT vanessadasilvaoliveira thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT sergiosantossilvajunior thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT edivaldodossantosrodrigues thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT claudiavaleriacamposdesouza thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT sabrinateixeiramartinez thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT osvaldoandradesantosfilho thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT cassiosantanameira thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT milenabotelhopereirasoares thealkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT jonatassousapiresdossantos alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT daharakeysecarvalhosilva alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT vanessadasilvaoliveira alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT sergiosantossilvajunior alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT edivaldodossantosrodrigues alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT claudiavaleriacamposdesouza alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT sabrinateixeiramartinez alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT osvaldoandradesantosfilho alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT cassiosantanameira alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor
AT milenabotelhopereirasoares alkaloidcaulerpinexhibitspotentandselectiveantiinflammatoryactivitythroughinteractionwiththeglucocorticoidreceptor