Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs
Paracoccidioidomycosis, a key issue for Brazilian health service, can be aggravated in patients with impaired immunological responses, such as diabetic patients. We evaluated the role of insulin in inflammatory parameters in diabetic and nondiabetic mice using a systemic mycosis Paracoccidioides bra...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/6209694 |
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| author | Felipe Beccaria Casagrande Sabrina de Souza Ferreira Fernanda Peixoto Barbosa Nunes Lavínia Maria Dal’Mas Romera Suelen Silvana dos Santos Fernando Henrique Galvão Tessaro Paula Regina Knox de Souza Sandro Rogério Almeida Joilson Oliveira Martins |
| author_facet | Felipe Beccaria Casagrande Sabrina de Souza Ferreira Fernanda Peixoto Barbosa Nunes Lavínia Maria Dal’Mas Romera Suelen Silvana dos Santos Fernando Henrique Galvão Tessaro Paula Regina Knox de Souza Sandro Rogério Almeida Joilson Oliveira Martins |
| author_sort | Felipe Beccaria Casagrande |
| collection | DOAJ |
| description | Paracoccidioidomycosis, a key issue for Brazilian health service, can be aggravated in patients with impaired immunological responses, such as diabetic patients. We evaluated the role of insulin in inflammatory parameters in diabetic and nondiabetic mice using a systemic mycosis Paracoccidioides brasiliensis (Pb) model. Diabetic C57BL-6 mice and controls were infected with Pb18 and treated with insulin for 12 days prior to experiments. After 55 days, infected diabetic mice exhibited fewer leukocytes in both peritoneal lavage fluid (PeLF) and bronchoalveolar lavage fluid and reduced secretion of interleukin- (IL-) 6 in lungs. In addition, diabetic mice presented a reduced influx of TCD4+ cells, TCD8+ cells, B lymphocytes, NK cells, and dendritic cells compared to control infected groups. Insulin treatment restored the leukocyte number in PeLF and restored the presence of B lymphocytes, dendritic cells, and NK cells in lungs of diabetic animals. The data suggest that diabetic mice present impaired immunological response to Pb18 infection and insulin modulates inflammation by reducing IL-6 levels in lung and CINC-1 levels in spleen and liver homogenates, restoring leukocyte concentrations in PeLF and also restoring populations of dendritic cells and B lymphocytes in lungs of diabetic mice, permitting the host to better control the infection. |
| format | Article |
| id | doaj-art-7e04e41548c84045b4c01bba3234d9cb |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-7e04e41548c84045b4c01bba3234d9cb2025-02-03T05:51:17ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/62096946209694Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in LungsFelipe Beccaria Casagrande0Sabrina de Souza Ferreira1Fernanda Peixoto Barbosa Nunes2Lavínia Maria Dal’Mas Romera3Suelen Silvana dos Santos4Fernando Henrique Galvão Tessaro5Paula Regina Knox de Souza6Sandro Rogério Almeida7Joilson Oliveira Martins8Laboratory of Immunoendocrinology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences of University Sao Paulo (FCF/USP), São Paulo, BrazilLaboratory of Immunoendocrinology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences of University Sao Paulo (FCF/USP), São Paulo, BrazilLaboratory of Immunoendocrinology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences of University Sao Paulo (FCF/USP), São Paulo, BrazilLaboratory of Mycology, Department of Clinical and Toxicological Analyses, FCF/USP, São Paulo, BrazilLaboratory of Mycology, Department of Clinical and Toxicological Analyses, FCF/USP, São Paulo, BrazilLaboratory of Immunoendocrinology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences of University Sao Paulo (FCF/USP), São Paulo, BrazilLaboratory of Immunoendocrinology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences of University Sao Paulo (FCF/USP), São Paulo, BrazilLaboratory of Mycology, Department of Clinical and Toxicological Analyses, FCF/USP, São Paulo, BrazilLaboratory of Immunoendocrinology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences of University Sao Paulo (FCF/USP), São Paulo, BrazilParacoccidioidomycosis, a key issue for Brazilian health service, can be aggravated in patients with impaired immunological responses, such as diabetic patients. We evaluated the role of insulin in inflammatory parameters in diabetic and nondiabetic mice using a systemic mycosis Paracoccidioides brasiliensis (Pb) model. Diabetic C57BL-6 mice and controls were infected with Pb18 and treated with insulin for 12 days prior to experiments. After 55 days, infected diabetic mice exhibited fewer leukocytes in both peritoneal lavage fluid (PeLF) and bronchoalveolar lavage fluid and reduced secretion of interleukin- (IL-) 6 in lungs. In addition, diabetic mice presented a reduced influx of TCD4+ cells, TCD8+ cells, B lymphocytes, NK cells, and dendritic cells compared to control infected groups. Insulin treatment restored the leukocyte number in PeLF and restored the presence of B lymphocytes, dendritic cells, and NK cells in lungs of diabetic animals. The data suggest that diabetic mice present impaired immunological response to Pb18 infection and insulin modulates inflammation by reducing IL-6 levels in lung and CINC-1 levels in spleen and liver homogenates, restoring leukocyte concentrations in PeLF and also restoring populations of dendritic cells and B lymphocytes in lungs of diabetic mice, permitting the host to better control the infection.http://dx.doi.org/10.1155/2018/6209694 |
| spellingShingle | Felipe Beccaria Casagrande Sabrina de Souza Ferreira Fernanda Peixoto Barbosa Nunes Lavínia Maria Dal’Mas Romera Suelen Silvana dos Santos Fernando Henrique Galvão Tessaro Paula Regina Knox de Souza Sandro Rogério Almeida Joilson Oliveira Martins Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs Journal of Diabetes Research |
| title | Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs |
| title_full | Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs |
| title_fullStr | Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs |
| title_full_unstemmed | Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs |
| title_short | Insulin Modulates Paracoccidioides brasiliensis-Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs |
| title_sort | insulin modulates paracoccidioides brasiliensis induced inflammation by restoring the populations of nk cells dendritic cells and b lymphocytes in lungs |
| url | http://dx.doi.org/10.1155/2018/6209694 |
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