<i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae)
Microbe–microbe interactions within a host drive shifts in the host’s microbiota composition, profoundly influencing host physiology, ecology, and evolution. Among these microbes, the maternally inherited endosymbiont <i>Wolbachia</i> is widespread in the invasive pest <i>Liriomyza...
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2025-01-01
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| author | Ya-Xin Duan Ying-Hua Zhuang Yu-Xin Wu Tian-Wei Huang Zhang-Rong Song Yu-Zhou Du Yu-Xi Zhu |
| author_facet | Ya-Xin Duan Ying-Hua Zhuang Yu-Xin Wu Tian-Wei Huang Zhang-Rong Song Yu-Zhou Du Yu-Xi Zhu |
| author_sort | Ya-Xin Duan |
| collection | DOAJ |
| description | Microbe–microbe interactions within a host drive shifts in the host’s microbiota composition, profoundly influencing host physiology, ecology, and evolution. Among these microbes, the maternally inherited endosymbiont <i>Wolbachia</i> is widespread in the invasive pest <i>Liriomyza huidorbrensis</i> (Diptera: Agromyzidae). However, its influence on the host microbiota remains largely unexplored. In the study presented herein, we investigated the bacterial communities of <i>Wolbachia</i> wLhui-infected (wLhui+) and -uninfected lines (wLhui−) of <i>L. huidorbrensis</i> using 16S rRNA gene high-throughput sequencing. For both leaf-miner lines, Bacteroidota was the dominant phylum (relative abundance: 59.18%), followed by Pseudomonadota (36.63%), Actinomycetota (2.42%), and Bacillota (0.93%). We found no significant differences in alpha-diversity indices between the wLhui+ and wLhui− lines (<i>p</i> > 0.05). However, principal coordinates analysis revealed significant differences in microbiota composition between the wLhui+ and wLhui− lines (PERMANOVA: <i>p</i> < 0.001), explaining 76.70% of the variance in microbiota composition. Correlation network analysis identified robust negative and positive associations between <i>Wolbachia</i> and several genera, suggesting that <i>Wolbachia</i> shapes microbial composition through competitive or cooperative interactions with specific taxa. Overall, our study suggests that <i>Wolbachia</i> plays a key role in shaping the leaf-miner microbiome, potentially affecting host fitness. |
| format | Article |
| id | doaj-art-7df22629ca6241098a1794dbd45fabc1 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-7df22629ca6241098a1794dbd45fabc12025-08-20T02:45:01ZengMDPI AGMicroorganisms2076-26072025-01-0113230210.3390/microorganisms13020302<i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae)Ya-Xin Duan0Ying-Hua Zhuang1Yu-Xin Wu2Tian-Wei Huang3Zhang-Rong Song4Yu-Zhou Du5Yu-Xi Zhu6Department of Entomology, College of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaDepartment of Entomology, College of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaDepartment of Entomology, College of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaDepartment of Entomology, College of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaEntomology and Nematology Department, University of Florida, Gainesville, FL 32611, USADepartment of Entomology, College of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaDepartment of Entomology, College of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaMicrobe–microbe interactions within a host drive shifts in the host’s microbiota composition, profoundly influencing host physiology, ecology, and evolution. Among these microbes, the maternally inherited endosymbiont <i>Wolbachia</i> is widespread in the invasive pest <i>Liriomyza huidorbrensis</i> (Diptera: Agromyzidae). However, its influence on the host microbiota remains largely unexplored. In the study presented herein, we investigated the bacterial communities of <i>Wolbachia</i> wLhui-infected (wLhui+) and -uninfected lines (wLhui−) of <i>L. huidorbrensis</i> using 16S rRNA gene high-throughput sequencing. For both leaf-miner lines, Bacteroidota was the dominant phylum (relative abundance: 59.18%), followed by Pseudomonadota (36.63%), Actinomycetota (2.42%), and Bacillota (0.93%). We found no significant differences in alpha-diversity indices between the wLhui+ and wLhui− lines (<i>p</i> > 0.05). However, principal coordinates analysis revealed significant differences in microbiota composition between the wLhui+ and wLhui− lines (PERMANOVA: <i>p</i> < 0.001), explaining 76.70% of the variance in microbiota composition. Correlation network analysis identified robust negative and positive associations between <i>Wolbachia</i> and several genera, suggesting that <i>Wolbachia</i> shapes microbial composition through competitive or cooperative interactions with specific taxa. Overall, our study suggests that <i>Wolbachia</i> plays a key role in shaping the leaf-miner microbiome, potentially affecting host fitness.https://www.mdpi.com/2076-2607/13/2/302endosymbiont<i>Wolbachia</i>invasive pestmicrobiome |
| spellingShingle | Ya-Xin Duan Ying-Hua Zhuang Yu-Xin Wu Tian-Wei Huang Zhang-Rong Song Yu-Zhou Du Yu-Xi Zhu <i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae) Microorganisms endosymbiont <i>Wolbachia</i> invasive pest microbiome |
| title | <i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae) |
| title_full | <i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae) |
| title_fullStr | <i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae) |
| title_full_unstemmed | <i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae) |
| title_short | <i>Wolbachia</i> Infection Alters the Microbiota of the Invasive Leaf-Miner <i>Liriomyza huidobrensis</i> (Diptera: Agromyzidae) |
| title_sort | i wolbachia i infection alters the microbiota of the invasive leaf miner i liriomyza huidobrensis i diptera agromyzidae |
| topic | endosymbiont <i>Wolbachia</i> invasive pest microbiome |
| url | https://www.mdpi.com/2076-2607/13/2/302 |
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