Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata
Objective: Investigating the in vitro anti-melanogenesis and anti-wrinkle effects of the fucoidan TO-F10 isolated from Turbinaria ornata. Methods: The colorimetric method was utilized to evaluate the inhibitory effects of TO-F10 at different concentrations on tyrosinase and collagenase in this study...
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The editorial department of Science and Technology of Food Industry
2025-07-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080230 |
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| author | Yifan ZHENG Fengqi YANG Xiaoting FU Xin GAO Lei WANG |
| author_facet | Yifan ZHENG Fengqi YANG Xiaoting FU Xin GAO Lei WANG |
| author_sort | Yifan ZHENG |
| collection | DOAJ |
| description | Objective: Investigating the in vitro anti-melanogenesis and anti-wrinkle effects of the fucoidan TO-F10 isolated from Turbinaria ornata. Methods: The colorimetric method was utilized to evaluate the inhibitory effects of TO-F10 at different concentrations on tyrosinase and collagenase in this study. The methyl thiazolyl tetrazolium (MTT) assay, DOPA oxidation assay, 2',7'-dichlorofluorescin diacetate (DCFH2-DA) assay, and enzyme-linked immunosorbent assay (ELISA) were used to examine the effects of TO-F10 on the cell viability, melanin content and tyrosinase activity in α-melanocyte-stimulating hormone (α-MSH)-induced mouse melanoma cells (B16F10), as well as its impact on the reactive oxygen species (ROS) level, the collagen content, matrix metalloproteinase (MMP) expression, and pro-inflammatory cytokine production in ultraviolet B (UVB)-induced human dermal fibroblasts (HDF). Results: At a concentration of 100 µg/mL, TO-F10 inhibited tyrosinase and collagenase by 26.98%±1.76% and 37.60%±0.60%, respectively. The increase in melanin content and decrease in tyrosinase activity in α-MSH-induced B16F10 cells were significantly reversed by TO-F10 treatment (P<0.01). UVB-exposed HDF cells were protected by TO-F10 through the inhibition of oxidative stress, collagen degradation, and inflammation. However, it significantly increased in cell viability were observed only at 50 and 100 µg/mL (P<0.05). Conclusion: TO-F10 was shown to possess anti-melanogenesis and anti-wrinkle properties, with its applicability in the functional food industry being indicated. |
| format | Article |
| id | doaj-art-7dedac0a2de94958b1c0ca86af309fd4 |
| institution | DOAJ |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-7dedac0a2de94958b1c0ca86af309fd42025-08-20T02:40:14ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-07-01461444144710.13386/j.issn1002-0306.20240802302024080230-14Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornataYifan ZHENG0Fengqi YANG1Xiaoting FU2Xin GAO3Lei WANG4College of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of KoreaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaObjective: Investigating the in vitro anti-melanogenesis and anti-wrinkle effects of the fucoidan TO-F10 isolated from Turbinaria ornata. Methods: The colorimetric method was utilized to evaluate the inhibitory effects of TO-F10 at different concentrations on tyrosinase and collagenase in this study. The methyl thiazolyl tetrazolium (MTT) assay, DOPA oxidation assay, 2',7'-dichlorofluorescin diacetate (DCFH2-DA) assay, and enzyme-linked immunosorbent assay (ELISA) were used to examine the effects of TO-F10 on the cell viability, melanin content and tyrosinase activity in α-melanocyte-stimulating hormone (α-MSH)-induced mouse melanoma cells (B16F10), as well as its impact on the reactive oxygen species (ROS) level, the collagen content, matrix metalloproteinase (MMP) expression, and pro-inflammatory cytokine production in ultraviolet B (UVB)-induced human dermal fibroblasts (HDF). Results: At a concentration of 100 µg/mL, TO-F10 inhibited tyrosinase and collagenase by 26.98%±1.76% and 37.60%±0.60%, respectively. The increase in melanin content and decrease in tyrosinase activity in α-MSH-induced B16F10 cells were significantly reversed by TO-F10 treatment (P<0.01). UVB-exposed HDF cells were protected by TO-F10 through the inhibition of oxidative stress, collagen degradation, and inflammation. However, it significantly increased in cell viability were observed only at 50 and 100 µg/mL (P<0.05). Conclusion: TO-F10 was shown to possess anti-melanogenesis and anti-wrinkle properties, with its applicability in the functional food industry being indicated.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080230turbinaria ornatafucoidanultraviolet b (uvb)anti-melanogenesisanti-wrinklephotodamage |
| spellingShingle | Yifan ZHENG Fengqi YANG Xiaoting FU Xin GAO Lei WANG Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata Shipin gongye ke-ji turbinaria ornata fucoidan ultraviolet b (uvb) anti-melanogenesis anti-wrinkle photodamage |
| title | Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata |
| title_full | Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata |
| title_fullStr | Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata |
| title_full_unstemmed | Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata |
| title_short | Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata |
| title_sort | anti melanogenesis and anti wrinkle effects of the fucoidan isolated from turbinaria ornata |
| topic | turbinaria ornata fucoidan ultraviolet b (uvb) anti-melanogenesis anti-wrinkle photodamage |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080230 |
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