Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice.
A high leaf vein density is both an essential feature of C4 photosynthesis and a foundation trait to C4 evolution, ensuring the optimal proportion and proximity of mesophyll and bundle sheath cells for permitting the rapid exchange of photosynthates. Two rice mutant populations, a deletion mutant li...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094947&type=printable |
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| author | Aryo B Feldman Erik H Murchie Hei Leung Marietta Baraoidan Robert Coe Su-May Yu Shuen-Fang Lo William P Quick |
| author_facet | Aryo B Feldman Erik H Murchie Hei Leung Marietta Baraoidan Robert Coe Su-May Yu Shuen-Fang Lo William P Quick |
| author_sort | Aryo B Feldman |
| collection | DOAJ |
| description | A high leaf vein density is both an essential feature of C4 photosynthesis and a foundation trait to C4 evolution, ensuring the optimal proportion and proximity of mesophyll and bundle sheath cells for permitting the rapid exchange of photosynthates. Two rice mutant populations, a deletion mutant library with a cv. IR64 background (12,470 lines) and a T-DNA insertion mutant library with a cv. Tainung 67 background (10,830 lines), were screened for increases in vein density. A high throughput method with handheld microscopes was developed and its accuracy was supported by more rigorous microscopy analysis. Eight lines with significantly increased leaf vein densities were identified to be used as genetic stock for the global C4 Rice Consortium. The candidate population was shown to include both shared and independent mutations and so more than one gene controlled the high vein density phenotype. The high vein density trait was found to be linked to a narrow leaf width trait but the linkage was incomplete. The more genetically robust narrow leaf width trait was proposed to be used as a reliable phenotypic marker for finding high vein density variants in rice in future screens. |
| format | Article |
| id | doaj-art-7dbfcc4b424f4ae49bb97f3277a2bf69 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7dbfcc4b424f4ae49bb97f3277a2bf692025-08-20T02:14:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9494710.1371/journal.pone.0094947Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice.Aryo B FeldmanErik H MurchieHei LeungMarietta BaraoidanRobert CoeSu-May YuShuen-Fang LoWilliam P QuickA high leaf vein density is both an essential feature of C4 photosynthesis and a foundation trait to C4 evolution, ensuring the optimal proportion and proximity of mesophyll and bundle sheath cells for permitting the rapid exchange of photosynthates. Two rice mutant populations, a deletion mutant library with a cv. IR64 background (12,470 lines) and a T-DNA insertion mutant library with a cv. Tainung 67 background (10,830 lines), were screened for increases in vein density. A high throughput method with handheld microscopes was developed and its accuracy was supported by more rigorous microscopy analysis. Eight lines with significantly increased leaf vein densities were identified to be used as genetic stock for the global C4 Rice Consortium. The candidate population was shown to include both shared and independent mutations and so more than one gene controlled the high vein density phenotype. The high vein density trait was found to be linked to a narrow leaf width trait but the linkage was incomplete. The more genetically robust narrow leaf width trait was proposed to be used as a reliable phenotypic marker for finding high vein density variants in rice in future screens.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094947&type=printable |
| spellingShingle | Aryo B Feldman Erik H Murchie Hei Leung Marietta Baraoidan Robert Coe Su-May Yu Shuen-Fang Lo William P Quick Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice. PLoS ONE |
| title | Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice. |
| title_full | Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice. |
| title_fullStr | Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice. |
| title_full_unstemmed | Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice. |
| title_short | Increasing leaf vein density by mutagenesis: laying the foundations for C4 rice. |
| title_sort | increasing leaf vein density by mutagenesis laying the foundations for c4 rice |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094947&type=printable |
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