Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)

Due to the advanced stage of polio eradication, the possible role of non-polio enteroviruses (NPEVs) associated to acute flaccid paralysis (AFP) cases has been highlighted. In this study, we described epidemiological aspects of NPEVs infections associated to AFP and explore the viral genetic diversi...

Full description

Saved in:
Bibliographic Details
Main Authors: Ivanildo P. Sousa, Maria de Lourdes Aguiar Oliveira, Fernanda M. Burlandy, Raiana S. Machado, Silas S. Oliveira, Fernando N. Tavares, Francisco Gomes-Neto, Eliane V. da Costa, Edson E. da Silva
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:Emerging Microbes and Infections
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2020.1850181
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850150296487985152
author Ivanildo P. Sousa
Maria de Lourdes Aguiar Oliveira
Fernanda M. Burlandy
Raiana S. Machado
Silas S. Oliveira
Fernando N. Tavares
Francisco Gomes-Neto
Eliane V. da Costa
Edson E. da Silva
author_facet Ivanildo P. Sousa
Maria de Lourdes Aguiar Oliveira
Fernanda M. Burlandy
Raiana S. Machado
Silas S. Oliveira
Fernando N. Tavares
Francisco Gomes-Neto
Eliane V. da Costa
Edson E. da Silva
author_sort Ivanildo P. Sousa
collection DOAJ
description Due to the advanced stage of polio eradication, the possible role of non-polio enteroviruses (NPEVs) associated to acute flaccid paralysis (AFP) cases has been highlighted. In this study, we described epidemiological aspects of NPEVs infections associated to AFP and explore the viral genetic diversity, information still scarce in Brazil. From 2005 to 2017, 6707 stool samples were collected in the scope of the Brazilian Poliomyelitis Surveillance Program. NPEVs were isolated in 359 samples (5.3%) and 341 (94.9%) were genotyped. About 46 different NPEV types were identified with the following detection pattern EV-B > EV-A > EV-C. The major EV-types were CVA2, CV4, EV-A71, CVB3, CVB5, E6, E7, E11, CVA13 and EV-C99, which corresponds to 51.6% of the total. Uncommon types, such as CVA12, EV-90 and CVA11, were also identified. Different E6 genogroups were observed, prevailing the GenIII, despite periods of co-circulation, and replacement of genogroups along time. CVA2 sequences were classified as genotype C and data suggested its dispersion in South-American countries. CVA13 viruses belonged to cluster B and Venezuelan viruses composed a new putative cluster. This study provides extensive information on enterovirus diversity associated with AFP, reinforcing the need of tailoring current surveillance strategies to timely monitor emergence/re-emergence of NPEVs.
format Article
id doaj-art-7dbfc094c10247749c26cad13e5c168b
institution OA Journals
issn 2222-1751
language English
publishDate 2020-01-01
publisher Taylor & Francis Group
record_format Article
series Emerging Microbes and Infections
spelling doaj-art-7dbfc094c10247749c26cad13e5c168b2025-08-20T02:26:36ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01912536254610.1080/22221751.2020.1850181Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)Ivanildo P. Sousa0Maria de Lourdes Aguiar Oliveira1Fernanda M. Burlandy2Raiana S. Machado3Silas S. Oliveira4Fernando N. Tavares5Francisco Gomes-Neto6Eliane V. da Costa7Edson E. da Silva8Laboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratorio de Vírus Respiratórios e do Sarampo, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Referência Regional em Enteroviroses, Seção de Virologia, Instituto Evandro Chagas, Ananindeua, BrazilLaboratório de Toxinologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilDue to the advanced stage of polio eradication, the possible role of non-polio enteroviruses (NPEVs) associated to acute flaccid paralysis (AFP) cases has been highlighted. In this study, we described epidemiological aspects of NPEVs infections associated to AFP and explore the viral genetic diversity, information still scarce in Brazil. From 2005 to 2017, 6707 stool samples were collected in the scope of the Brazilian Poliomyelitis Surveillance Program. NPEVs were isolated in 359 samples (5.3%) and 341 (94.9%) were genotyped. About 46 different NPEV types were identified with the following detection pattern EV-B > EV-A > EV-C. The major EV-types were CVA2, CV4, EV-A71, CVB3, CVB5, E6, E7, E11, CVA13 and EV-C99, which corresponds to 51.6% of the total. Uncommon types, such as CVA12, EV-90 and CVA11, were also identified. Different E6 genogroups were observed, prevailing the GenIII, despite periods of co-circulation, and replacement of genogroups along time. CVA2 sequences were classified as genotype C and data suggested its dispersion in South-American countries. CVA13 viruses belonged to cluster B and Venezuelan viruses composed a new putative cluster. This study provides extensive information on enterovirus diversity associated with AFP, reinforcing the need of tailoring current surveillance strategies to timely monitor emergence/re-emergence of NPEVs.https://www.tandfonline.com/doi/10.1080/22221751.2020.1850181Non-polio enterovirusacute flaccid paralysisenterovirusmolecular epidemiologypoliomyelitis surveillance
spellingShingle Ivanildo P. Sousa
Maria de Lourdes Aguiar Oliveira
Fernanda M. Burlandy
Raiana S. Machado
Silas S. Oliveira
Fernando N. Tavares
Francisco Gomes-Neto
Eliane V. da Costa
Edson E. da Silva
Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)
Emerging Microbes and Infections
Non-polio enterovirus
acute flaccid paralysis
enterovirus
molecular epidemiology
poliomyelitis surveillance
title Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)
title_full Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)
title_fullStr Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)
title_full_unstemmed Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)
title_short Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: a historical series (2005–2017)
title_sort molecular characterization and epidemiological aspects of non polio enteroviruses isolated from acute flaccid paralysis in brazil a historical series 2005 2017
topic Non-polio enterovirus
acute flaccid paralysis
enterovirus
molecular epidemiology
poliomyelitis surveillance
url https://www.tandfonline.com/doi/10.1080/22221751.2020.1850181
work_keys_str_mv AT ivanildopsousa molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT mariadelourdesaguiaroliveira molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT fernandamburlandy molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT raianasmachado molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT silassoliveira molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT fernandontavares molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT franciscogomesneto molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT elianevdacosta molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017
AT edsonedasilva molecularcharacterizationandepidemiologicalaspectsofnonpolioenterovirusesisolatedfromacuteflaccidparalysisinbrazilahistoricalseries20052017