Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood
Pregnant women and their fetuses, a very fragile population, are exposed to polycyclic aromatic hydrocarbons (PAHs) and subject to potentially serious health risks. Consequently, the determination of PAHs in maternal serum and umbilical cord blood is necessary but challenging, due to their highly va...
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Académie des sciences
2024-02-01
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| Series: | Comptes Rendus. Chimie |
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| Online Access: | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.286/ |
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| author | Swiha, Stéphanie Pichon, Valérie Fournier, Thierry Gil, Sophie Delaunay, Nathalie |
| author_facet | Swiha, Stéphanie Pichon, Valérie Fournier, Thierry Gil, Sophie Delaunay, Nathalie |
| author_sort | Swiha, Stéphanie |
| collection | DOAJ |
| description | Pregnant women and their fetuses, a very fragile population, are exposed to polycyclic aromatic hydrocarbons (PAHs) and subject to potentially serious health risks. Consequently, the determination of PAHs in maternal serum and umbilical cord blood is necessary but challenging, due to their highly varied physico-chemical properties and trace presence. The aim of this project was to develop a method enabling, for the first time, the simultaneous determination of 22 listed PAHs from small volumes (100 ${\mu }$L) of maternal serum and umbilical cord blood, including a sample pre-treatment reducing as far as possible the quantity of solvents used. A solid-phase extraction step on C18-grafted silica was optimized by studying the effect of different parameters (nature and proportion of organic solvent added to the sample, washing and elution conditions) on extraction yields. The evaporation step was carefully controlled to limit the loss of the most volatile PAHs. Then, to break any interactions between PAHs and serum proteins, a precipitation step prior to solid-phase extraction was optimized using a design of experiments. The final sample processing procedure led to extraction yields of between 27 and 57% and between 34 and 69% for 22 PAHs in serum and umbilical cord blood, respectively, with standard deviation values of less than 11%. Limits of quantification between 0.2 and 3.1 ${\mu }$g/L were achieved for PAHs fluorescing in spiked real samples, close to the concentrations expected in biological samples. |
| format | Article |
| id | doaj-art-7d93c6134f6347d09f96f05844cea9ad |
| institution | Kabale University |
| issn | 1878-1543 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Académie des sciences |
| record_format | Article |
| series | Comptes Rendus. Chimie |
| spelling | doaj-art-7d93c6134f6347d09f96f05844cea9ad2025-02-07T13:40:29ZengAcadémie des sciencesComptes Rendus. Chimie1878-15432024-02-0127S219721210.5802/crchim.28610.5802/crchim.286Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord bloodSwiha, Stéphanie0Pichon, Valérie1Fournier, Thierry2Gil, Sophie3Delaunay, Nathalie4https://orcid.org/0000-0003-3008-0286INSERM U1139, Faculté de Pharmacie, Paris, France; Laboratory of Analytical and Bioanalytical Sciences and Miniaturization, UMR CBI 8231 CNRS – ESPCI Paris, PSL University, Paris, FranceLaboratory of Analytical and Bioanalytical Sciences and Miniaturization, UMR CBI 8231 CNRS – ESPCI Paris, PSL University, Paris, France; Sorbonne Université, Paris, FranceFondation PremUP, Paris, France; INSERM U1139, Faculté de Pharmacie, Paris, France; Université Paris Descartes, UMR-S 1139, Sorbonne Paris Cité, Paris, FranceFondation PremUP, Paris, France; INSERM U1139, Faculté de Pharmacie, Paris, France; Université Paris Descartes, UMR-S 1139, Sorbonne Paris Cité, Paris, FranceLaboratory of Analytical and Bioanalytical Sciences and Miniaturization, UMR CBI 8231 CNRS – ESPCI Paris, PSL University, Paris, FrancePregnant women and their fetuses, a very fragile population, are exposed to polycyclic aromatic hydrocarbons (PAHs) and subject to potentially serious health risks. Consequently, the determination of PAHs in maternal serum and umbilical cord blood is necessary but challenging, due to their highly varied physico-chemical properties and trace presence. The aim of this project was to develop a method enabling, for the first time, the simultaneous determination of 22 listed PAHs from small volumes (100 ${\mu }$L) of maternal serum and umbilical cord blood, including a sample pre-treatment reducing as far as possible the quantity of solvents used. A solid-phase extraction step on C18-grafted silica was optimized by studying the effect of different parameters (nature and proportion of organic solvent added to the sample, washing and elution conditions) on extraction yields. The evaporation step was carefully controlled to limit the loss of the most volatile PAHs. Then, to break any interactions between PAHs and serum proteins, a precipitation step prior to solid-phase extraction was optimized using a design of experiments. The final sample processing procedure led to extraction yields of between 27 and 57% and between 34 and 69% for 22 PAHs in serum and umbilical cord blood, respectively, with standard deviation values of less than 11%. Limits of quantification between 0.2 and 3.1 ${\mu }$g/L were achieved for PAHs fluorescing in spiked real samples, close to the concentrations expected in biological samples.https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.286/Polycyclic aromatic hydrocarbonsMaternal serumUmbilical cord bloodSolid phase extractionLiquid chromatography |
| spellingShingle | Swiha, Stéphanie Pichon, Valérie Fournier, Thierry Gil, Sophie Delaunay, Nathalie Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood Comptes Rendus. Chimie Polycyclic aromatic hydrocarbons Maternal serum Umbilical cord blood Solid phase extraction Liquid chromatography |
| title | Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood |
| title_full | Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood |
| title_fullStr | Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood |
| title_full_unstemmed | Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood |
| title_short | Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood |
| title_sort | development of an analytical method for the simultaneous determination of 22 polycyclic aromatic hydrocarbons pahs in maternal and umbilical cord blood |
| topic | Polycyclic aromatic hydrocarbons Maternal serum Umbilical cord blood Solid phase extraction Liquid chromatography |
| url | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.286/ |
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